Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3319 and Pf6N2E2_3320 are separated by 12 nucleotides Pf6N2E2_3320 and Pf6N2E2_3321 are separated by 53 nucleotides
Pf6N2E2_3319 - Peptide synthetase, at 3,835,014 to 3,836,642
_3319
Pf6N2E2_3320 - hypothetical protein, at 3,836,655 to 3,836,774
_3320
Pf6N2E2_3321 - Ornithine cyclodeaminase (EC 4.3.1.12), at 3,836,828 to 3,837,781
_3321
Pf6N2E2_3322 - Peptide synthetase, at 3,837,738 to 3,843,569
_3322
Position (kb)
3836
3837 Strain fitness (log2 ratio)
-2
-1
0
1 at 3835.668 kb on - strand, within Pf6N2E2_3319 at 3835.702 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.732 kb on + strand, within Pf6N2E2_3319 at 3835.733 kb on - strand, within Pf6N2E2_3319 at 3835.733 kb on - strand, within Pf6N2E2_3319 at 3835.733 kb on - strand, within Pf6N2E2_3319 at 3835.964 kb on + strand, within Pf6N2E2_3319 at 3835.964 kb on + strand, within Pf6N2E2_3319 at 3835.964 kb on + strand, within Pf6N2E2_3319 at 3835.965 kb on - strand, within Pf6N2E2_3319 at 3835.965 kb on - strand, within Pf6N2E2_3319 at 3835.965 kb on - strand, within Pf6N2E2_3319 at 3836.200 kb on + strand, within Pf6N2E2_3319 at 3836.200 kb on + strand, within Pf6N2E2_3319 at 3836.200 kb on + strand, within Pf6N2E2_3319 at 3836.201 kb on - strand, within Pf6N2E2_3319 at 3836.271 kb on - strand, within Pf6N2E2_3319 at 3836.293 kb on + strand, within Pf6N2E2_3319 at 3836.293 kb on + strand, within Pf6N2E2_3319 at 3836.293 kb on + strand, within Pf6N2E2_3319 at 3836.294 kb on - strand, within Pf6N2E2_3319 at 3836.294 kb on - strand, within Pf6N2E2_3319 at 3836.294 kb on - strand, within Pf6N2E2_3319 at 3836.414 kb on - strand, within Pf6N2E2_3319 at 3836.461 kb on - strand, within Pf6N2E2_3319 at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.529 kb on + strand at 3836.530 kb on - strand at 3836.530 kb on - strand at 3836.530 kb on - strand at 3836.530 kb on - strand at 3836.530 kb on - strand at 3836.530 kb on - strand at 3836.611 kb on + strand at 3836.612 kb on - strand at 3836.650 kb on + strand at 3836.650 kb on + strand at 3836.651 kb on - strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.654 kb on + strand at 3836.655 kb on - strand at 3836.655 kb on - strand at 3836.655 kb on - strand at 3836.676 kb on + strand, within Pf6N2E2_3320 at 3836.677 kb on - strand, within Pf6N2E2_3320 at 3836.706 kb on - strand, within Pf6N2E2_3320 at 3836.731 kb on - strand, within Pf6N2E2_3320 at 3836.731 kb on - strand, within Pf6N2E2_3320 at 3836.781 kb on - strand at 3836.781 kb on - strand at 3836.781 kb on - strand at 3836.850 kb on + strand at 3836.850 kb on + strand at 3836.851 kb on - strand at 3836.851 kb on - strand at 3836.851 kb on - strand at 3836.851 kb on - strand at 3836.851 kb on - strand at 3836.893 kb on - strand at 3836.991 kb on - strand, within Pf6N2E2_3321 at 3837.092 kb on + strand, within Pf6N2E2_3321 at 3837.159 kb on - strand, within Pf6N2E2_3321 at 3837.206 kb on + strand, within Pf6N2E2_3321 at 3837.207 kb on - strand, within Pf6N2E2_3321 at 3837.207 kb on - strand, within Pf6N2E2_3321 at 3837.330 kb on + strand, within Pf6N2E2_3321 at 3837.331 kb on - strand, within Pf6N2E2_3321 at 3837.331 kb on - strand, within Pf6N2E2_3321 at 3837.361 kb on - strand, within Pf6N2E2_3321 at 3837.481 kb on - strand, within Pf6N2E2_3321 at 3837.481 kb on - strand, within Pf6N2E2_3321 at 3837.536 kb on - strand, within Pf6N2E2_3321 at 3837.678 kb on - strand, within Pf6N2E2_3321 at 3837.714 kb on + strand at 3837.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,835,668 - Pf6N2E2_3319 0.40 +0.2 3,835,702 + Pf6N2E2_3319 0.42 -0.4 3,835,732 + Pf6N2E2_3319 0.44 -0.0 3,835,732 + Pf6N2E2_3319 0.44 -0.8 3,835,732 + Pf6N2E2_3319 0.44 -0.1 3,835,732 + Pf6N2E2_3319 0.44 +0.7 3,835,732 + Pf6N2E2_3319 0.44 +0.6 3,835,732 + Pf6N2E2_3319 0.44 -0.4 3,835,733 - Pf6N2E2_3319 0.44 -1.1 3,835,733 - Pf6N2E2_3319 0.44 +0.0 3,835,733 - Pf6N2E2_3319 0.44 +0.4 3,835,964 + Pf6N2E2_3319 0.58 +0.6 3,835,964 + Pf6N2E2_3319 0.58 -0.1 3,835,964 + Pf6N2E2_3319 0.58 -0.6 3,835,965 - Pf6N2E2_3319 0.58 +0.6 3,835,965 - Pf6N2E2_3319 0.58 +0.6 3,835,965 - Pf6N2E2_3319 0.58 +0.7 3,836,200 + Pf6N2E2_3319 0.73 -0.2 3,836,200 + Pf6N2E2_3319 0.73 +0.6 3,836,200 + Pf6N2E2_3319 0.73 -0.2 3,836,201 - Pf6N2E2_3319 0.73 +0.1 3,836,271 - Pf6N2E2_3319 0.77 -0.3 3,836,293 + Pf6N2E2_3319 0.79 -0.5 3,836,293 + Pf6N2E2_3319 0.79 -0.0 3,836,293 + Pf6N2E2_3319 0.79 +0.4 3,836,294 - Pf6N2E2_3319 0.79 -0.9 3,836,294 - Pf6N2E2_3319 0.79 +1.4 3,836,294 - Pf6N2E2_3319 0.79 -0.2 3,836,414 - Pf6N2E2_3319 0.86 -0.0 3,836,461 - Pf6N2E2_3319 0.89 +0.2 3,836,529 + +0.6 3,836,529 + -0.2 3,836,529 + +0.7 3,836,529 + +0.5 3,836,529 + +1.3 3,836,529 + +0.3 3,836,529 + -0.4 3,836,529 + +0.2 3,836,529 + -0.7 3,836,530 - +0.6 3,836,530 - -0.3 3,836,530 - +0.0 3,836,530 - +0.8 3,836,530 - -0.6 3,836,530 - -0.1 3,836,611 + +0.2 3,836,612 - +0.6 3,836,650 + -0.0 3,836,650 + +0.3 3,836,651 - +0.5 3,836,654 + -0.5 3,836,654 + +0.2 3,836,654 + -0.1 3,836,654 + +0.3 3,836,654 + -1.8 3,836,654 + -0.1 3,836,654 + +0.1 3,836,654 + -0.3 3,836,655 - +0.4 3,836,655 - +0.2 3,836,655 - +0.1 3,836,676 + Pf6N2E2_3320 0.17 +0.1 3,836,677 - Pf6N2E2_3320 0.18 +0.1 3,836,706 - Pf6N2E2_3320 0.42 -0.7 3,836,731 - Pf6N2E2_3320 0.63 -0.1 3,836,731 - Pf6N2E2_3320 0.63 -1.1 3,836,781 - +0.4 3,836,781 - +0.0 3,836,781 - -0.7 3,836,850 + +0.4 3,836,850 + +0.3 3,836,851 - +1.2 3,836,851 - +0.7 3,836,851 - -0.1 3,836,851 - +0.6 3,836,851 - +0.6 3,836,893 - -0.0 3,836,991 - Pf6N2E2_3321 0.17 +0.3 3,837,092 + Pf6N2E2_3321 0.28 -0.3 3,837,159 - Pf6N2E2_3321 0.35 -0.3 3,837,206 + Pf6N2E2_3321 0.40 +0.1 3,837,207 - Pf6N2E2_3321 0.40 -0.2 3,837,207 - Pf6N2E2_3321 0.40 -0.7 3,837,330 + Pf6N2E2_3321 0.53 +0.3 3,837,331 - Pf6N2E2_3321 0.53 -0.6 3,837,331 - Pf6N2E2_3321 0.53 +1.2 3,837,361 - Pf6N2E2_3321 0.56 +0.1 3,837,481 - Pf6N2E2_3321 0.68 +0.8 3,837,481 - Pf6N2E2_3321 0.68 +0.6 3,837,536 - Pf6N2E2_3321 0.74 -0.2 3,837,678 - Pf6N2E2_3321 0.89 +0.4 3,837,714 + -0.4 3,837,715 - +1.4
Or see this region's nucleotide sequence