Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_314

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_313 and Pf6N2E2_314 are separated by 40 nucleotidesPf6N2E2_314 and Pf6N2E2_315 are separated by 193 nucleotides Pf6N2E2_313 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 379,362 to 381,089 _313 Pf6N2E2_314 - Isochorismatase (EC 3.3.2.1), at 381,130 to 381,663 _314 Pf6N2E2_315 - probable transmembrane protein, at 381,857 to 382,717 _315 Position (kb) 381 382Strain fitness (log2 ratio) -1 0 1at 380.163 kb on + strand, within Pf6N2E2_313at 380.292 kb on + strand, within Pf6N2E2_313at 380.320 kb on - strand, within Pf6N2E2_313at 380.337 kb on + strand, within Pf6N2E2_313at 380.337 kb on + strand, within Pf6N2E2_313at 380.338 kb on - strand, within Pf6N2E2_313at 380.387 kb on + strand, within Pf6N2E2_313at 380.497 kb on - strand, within Pf6N2E2_313at 380.538 kb on + strand, within Pf6N2E2_313at 380.538 kb on + strand, within Pf6N2E2_313at 380.538 kb on + strand, within Pf6N2E2_313at 380.538 kb on + strand, within Pf6N2E2_313at 380.539 kb on - strand, within Pf6N2E2_313at 380.539 kb on - strand, within Pf6N2E2_313at 380.646 kb on + strand, within Pf6N2E2_313at 380.647 kb on - strand, within Pf6N2E2_313at 380.647 kb on - strand, within Pf6N2E2_313at 380.799 kb on - strand, within Pf6N2E2_313at 380.817 kb on + strand, within Pf6N2E2_313at 381.039 kb on + strandat 381.039 kb on + strandat 381.040 kb on - strandat 381.040 kb on - strandat 381.040 kb on - strandat 381.040 kb on - strandat 381.040 kb on - strandat 381.304 kb on + strand, within Pf6N2E2_314at 381.304 kb on + strand, within Pf6N2E2_314at 381.304 kb on + strand, within Pf6N2E2_314at 381.304 kb on + strand, within Pf6N2E2_314at 381.304 kb on + strand, within Pf6N2E2_314at 381.305 kb on - strand, within Pf6N2E2_314at 381.305 kb on - strand, within Pf6N2E2_314at 381.305 kb on - strand, within Pf6N2E2_314at 381.305 kb on - strand, within Pf6N2E2_314at 381.392 kb on - strand, within Pf6N2E2_314at 381.661 kb on + strandat 381.682 kb on - strandat 381.682 kb on - strandat 381.691 kb on + strandat 381.885 kb on + strandat 381.885 kb on + strandat 381.886 kb on - strandat 381.886 kb on - strandat 382.042 kb on + strand, within Pf6N2E2_315at 382.043 kb on - strand, within Pf6N2E2_315at 382.043 kb on - strand, within Pf6N2E2_315at 382.043 kb on - strand, within Pf6N2E2_315at 382.262 kb on - strand, within Pf6N2E2_315at 382.359 kb on + strand, within Pf6N2E2_315at 382.360 kb on - strand, within Pf6N2E2_315at 382.360 kb on - strand, within Pf6N2E2_315at 382.655 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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380,163 + Pf6N2E2_313 0.46 -0.2
380,292 + Pf6N2E2_313 0.54 -0.3
380,320 - Pf6N2E2_313 0.55 +1.2
380,337 + Pf6N2E2_313 0.56 +1.3
380,337 + Pf6N2E2_313 0.56 +0.3
380,338 - Pf6N2E2_313 0.56 +0.4
380,387 + Pf6N2E2_313 0.59 +0.3
380,497 - Pf6N2E2_313 0.66 +0.7
380,538 + Pf6N2E2_313 0.68 +1.4
380,538 + Pf6N2E2_313 0.68 -0.1
380,538 + Pf6N2E2_313 0.68 -0.4
380,538 + Pf6N2E2_313 0.68 +1.0
380,539 - Pf6N2E2_313 0.68 +0.7
380,539 - Pf6N2E2_313 0.68 -0.9
380,646 + Pf6N2E2_313 0.74 -1.1
380,647 - Pf6N2E2_313 0.74 +0.2
380,647 - Pf6N2E2_313 0.74 +0.6
380,799 - Pf6N2E2_313 0.83 -0.3
380,817 + Pf6N2E2_313 0.84 +0.6
381,039 + +0.3
381,039 + +0.1
381,040 - +0.1
381,040 - -0.4
381,040 - -0.2
381,040 - +0.1
381,040 - +0.2
381,304 + Pf6N2E2_314 0.33 +0.0
381,304 + Pf6N2E2_314 0.33 -0.2
381,304 + Pf6N2E2_314 0.33 -0.7
381,304 + Pf6N2E2_314 0.33 +0.5
381,304 + Pf6N2E2_314 0.33 -0.4
381,305 - Pf6N2E2_314 0.33 +0.1
381,305 - Pf6N2E2_314 0.33 -0.4
381,305 - Pf6N2E2_314 0.33 -1.4
381,305 - Pf6N2E2_314 0.33 -0.2
381,392 - Pf6N2E2_314 0.49 -0.5
381,661 + -0.6
381,682 - +0.3
381,682 - -0.3
381,691 + +0.1
381,885 + +0.6
381,885 + +0.6
381,886 - +0.2
381,886 - -0.5
382,042 + Pf6N2E2_315 0.21 -0.8
382,043 - Pf6N2E2_315 0.22 -0.8
382,043 - Pf6N2E2_315 0.22 +0.6
382,043 - Pf6N2E2_315 0.22 -0.9
382,262 - Pf6N2E2_315 0.47 +0.1
382,359 + Pf6N2E2_315 0.58 +0.3
382,360 - Pf6N2E2_315 0.58 -0.1
382,360 - Pf6N2E2_315 0.58 +0.5
382,655 + -0.4

Or see this region's nucleotide sequence