Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03755 and PFLU_RS03760 are separated by 101 nucleotides PFLU_RS03760 and PFLU_RS31400 overlap by 26 nucleotides PFLU_RS31400 and PFLU_RS03765 overlap by 21 nucleotides PFLU_RS03765 and PFLU_RS03770 are separated by 9 nucleotides
PFLU_RS03755: PFLU_RS03755 - PDZ domain-containing protein, at 857,660 to 858,814
_RS03755
PFLU_RS03760: PFLU_RS03760 - Nif3-like dinuclear metal center hexameric protein, at 858,916 to 859,674
_RS03760
PFLU_RS31400: PFLU_RS31400 - hypothetical protein, at 859,649 to 859,831
_RS31400
PFLU_RS03765: PFLU_RS03765 - sulfate adenylyltransferase subunit CysD, at 859,811 to 860,728
_RS03765
PFLU_RS03770: PFLU_RS03770 - sulfate adenylyltransferase subunit CysN, at 860,738 to 862,636
_RS03770
Position (kb) 859
860 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 858.651 kb on + strand, within PFLU_RS03755 at 858.651 kb on + strand, within PFLU_RS03755 at 858.651 kb on + strand, within PFLU_RS03755 at 858.651 kb on + strand, within PFLU_RS03755 at 858.652 kb on - strand, within PFLU_RS03755 at 858.652 kb on - strand, within PFLU_RS03755 at 858.652 kb on - strand, within PFLU_RS03755 at 858.720 kb on - strand at 858.720 kb on - strand at 858.748 kb on + strand at 858.748 kb on + strand at 858.748 kb on + strand at 858.819 kb on + strand at 858.820 kb on - strand at 858.820 kb on - strand at 858.820 kb on - strand at 858.820 kb on - strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.870 kb on + strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.871 kb on - strand at 858.872 kb on + strand at 858.872 kb on + strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.873 kb on - strand at 858.901 kb on - strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.904 kb on + strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.905 kb on - strand at 858.915 kb on + strand at 858.916 kb on - strand at 858.916 kb on - strand at 858.986 kb on - strand at 859.117 kb on + strand, within PFLU_RS03760 at 859.146 kb on + strand, within PFLU_RS03760 at 859.308 kb on + strand, within PFLU_RS03760 at 859.309 kb on - strand, within PFLU_RS03760 at 859.309 kb on - strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.462 kb on + strand, within PFLU_RS03760 at 859.463 kb on - strand, within PFLU_RS03760 at 859.463 kb on - strand, within PFLU_RS03760 at 859.463 kb on - strand, within PFLU_RS03760 at 859.463 kb on - strand, within PFLU_RS03760 at 859.615 kb on + strand at 859.615 kb on + strand at 859.615 kb on + strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.616 kb on - strand at 859.693 kb on + strand, within PFLU_RS31400 at 859.693 kb on + strand, within PFLU_RS31400 at 859.693 kb on + strand, within PFLU_RS31400 at 859.693 kb on + strand, within PFLU_RS31400 at 859.694 kb on - strand, within PFLU_RS31400 at 859.694 kb on - strand, within PFLU_RS31400 at 859.699 kb on + strand, within PFLU_RS31400 at 859.699 kb on + strand, within PFLU_RS31400 at 859.699 kb on + strand, within PFLU_RS31400 at 859.700 kb on - strand, within PFLU_RS31400 at 859.700 kb on - strand, within PFLU_RS31400 at 859.700 kb on - strand, within PFLU_RS31400 at 859.701 kb on + strand, within PFLU_RS31400 at 859.701 kb on + strand, within PFLU_RS31400 at 859.702 kb on - strand, within PFLU_RS31400 at 859.714 kb on + strand, within PFLU_RS31400 at 859.714 kb on + strand, within PFLU_RS31400 at 859.730 kb on + strand, within PFLU_RS31400 at 859.730 kb on + strand, within PFLU_RS31400 at 859.731 kb on - strand, within PFLU_RS31400 at 859.731 kb on - strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.748 kb on + strand, within PFLU_RS31400 at 859.749 kb on - strand, within PFLU_RS31400 at 859.749 kb on - strand, within PFLU_RS31400 at 859.749 kb on - strand, within PFLU_RS31400 at 859.749 kb on - strand, within PFLU_RS31400 at 859.786 kb on + strand, within PFLU_RS31400 at 859.786 kb on + strand, within PFLU_RS31400 at 859.786 kb on + strand, within PFLU_RS31400 at 859.786 kb on + strand, within PFLU_RS31400 at 859.786 kb on + strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.787 kb on - strand, within PFLU_RS31400 at 859.806 kb on + strand, within PFLU_RS31400 at 859.806 kb on + strand, within PFLU_RS31400 at 859.806 kb on + strand, within PFLU_RS31400 at 859.807 kb on - strand, within PFLU_RS31400 at 859.807 kb on - strand, within PFLU_RS31400 at 859.807 kb on - strand, within PFLU_RS31400 at 859.907 kb on + strand, within PFLU_RS03765 at 859.907 kb on + strand, within PFLU_RS03765 at 859.908 kb on - strand, within PFLU_RS03765 at 859.908 kb on - strand, within PFLU_RS03765 at 859.918 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.024 kb on + strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.025 kb on - strand, within PFLU_RS03765 at 860.072 kb on - strand, within PFLU_RS03765 at 860.144 kb on - strand, within PFLU_RS03765 at 860.252 kb on + strand, within PFLU_RS03765 at 860.318 kb on + strand, within PFLU_RS03765 at 860.318 kb on + strand, within PFLU_RS03765 at 860.319 kb on - strand, within PFLU_RS03765 at 860.319 kb on - strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.396 kb on + strand, within PFLU_RS03765 at 860.397 kb on - strand, within PFLU_RS03765 at 860.397 kb on - strand, within PFLU_RS03765 at 860.397 kb on - strand, within PFLU_RS03765 at 860.397 kb on - strand, within PFLU_RS03765 at 860.397 kb on - strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.402 kb on + strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.403 kb on - strand, within PFLU_RS03765 at 860.424 kb on + strand, within PFLU_RS03765 at 860.424 kb on + strand, within PFLU_RS03765 at 860.425 kb on - strand, within PFLU_RS03765 at 860.432 kb on + strand, within PFLU_RS03765 at 860.433 kb on - strand, within PFLU_RS03765 at 860.433 kb on - strand, within PFLU_RS03765 at 860.533 kb on + strand, within PFLU_RS03765 at 860.560 kb on + strand, within PFLU_RS03765 at 860.704 kb on + strand at 860.704 kb on + strand at 860.705 kb on - strand at 860.720 kb on + strand at 860.721 kb on - strand at 860.726 kb on + strand at 860.727 kb on - strand at 860.727 kb on - strand at 860.727 kb on - strand at 860.780 kb on + strand at 860.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 858,651 + PFLU_RS03755 0.86 -0.4 858,651 + PFLU_RS03755 0.86 +3.7 858,651 + PFLU_RS03755 0.86 -2.0 858,651 + PFLU_RS03755 0.86 -1.1 858,652 - PFLU_RS03755 0.86 -1.2 858,652 - PFLU_RS03755 0.86 -0.8 858,652 - PFLU_RS03755 0.86 -0.8 858,720 - -1.3 858,720 - -0.5 858,748 + +2.6 858,748 + -2.2 858,748 + +0.3 858,819 + -1.5 858,820 - -2.2 858,820 - +0.4 858,820 - +2.5 858,820 - -0.4 858,870 + +3.6 858,870 + -0.5 858,870 + +1.2 858,870 + -2.0 858,870 + +0.1 858,870 + -0.8 858,870 + +1.5 858,870 + +0.3 858,871 - -1.9 858,871 - -0.2 858,871 - -1.0 858,871 - +2.4 858,871 - +0.5 858,871 - -1.4 858,871 - -0.7 858,871 - +1.5 858,871 - +2.6 858,871 - -2.0 858,872 + -1.5 858,872 + +1.5 858,873 - +4.0 858,873 - -0.3 858,873 - +1.2 858,873 - +0.3 858,873 - -0.5 858,873 - -2.2 858,873 - +0.6 858,873 - +0.4 858,873 - +2.0 858,873 - +0.0 858,901 - -1.1 858,904 + -0.8 858,904 + +1.6 858,904 + +1.3 858,904 + +0.3 858,904 + -1.5 858,904 + -0.1 858,904 + +0.9 858,904 + -0.2 858,905 - +2.3 858,905 - +0.3 858,905 - -2.0 858,905 - -2.0 858,905 - +3.1 858,905 - -1.2 858,905 - +2.8 858,905 - +1.0 858,905 - +1.9 858,905 - +0.2 858,905 - -1.5 858,915 + -2.0 858,916 - -0.5 858,916 - -1.2 858,986 - +3.4 859,117 + PFLU_RS03760 0.26 +0.4 859,146 + PFLU_RS03760 0.30 -0.4 859,308 + PFLU_RS03760 0.52 -1.4 859,309 - PFLU_RS03760 0.52 +4.1 859,309 - PFLU_RS03760 0.52 -1.5 859,462 + PFLU_RS03760 0.72 +2.3 859,462 + PFLU_RS03760 0.72 -1.1 859,462 + PFLU_RS03760 0.72 +3.1 859,462 + PFLU_RS03760 0.72 +0.5 859,462 + PFLU_RS03760 0.72 -0.1 859,462 + PFLU_RS03760 0.72 +2.1 859,463 - PFLU_RS03760 0.72 +1.0 859,463 - PFLU_RS03760 0.72 +0.9 859,463 - PFLU_RS03760 0.72 -2.3 859,463 - PFLU_RS03760 0.72 +1.4 859,615 + -0.5 859,615 + +2.8 859,615 + +2.3 859,616 - -0.5 859,616 - -0.8 859,616 - +1.0 859,616 - -1.3 859,616 - +2.2 859,616 - +2.2 859,616 - -0.3 859,693 + PFLU_RS31400 0.24 -0.4 859,693 + PFLU_RS31400 0.24 +1.0 859,693 + PFLU_RS31400 0.24 -1.4 859,693 + PFLU_RS31400 0.24 -1.2 859,694 - PFLU_RS31400 0.25 -1.0 859,694 - PFLU_RS31400 0.25 -1.4 859,699 + PFLU_RS31400 0.27 -2.1 859,699 + PFLU_RS31400 0.27 +0.3 859,699 + PFLU_RS31400 0.27 -1.5 859,700 - PFLU_RS31400 0.28 +0.5 859,700 - PFLU_RS31400 0.28 -1.8 859,700 - PFLU_RS31400 0.28 -0.2 859,701 + PFLU_RS31400 0.28 +0.6 859,701 + PFLU_RS31400 0.28 -1.2 859,702 - PFLU_RS31400 0.29 -0.2 859,714 + PFLU_RS31400 0.36 -0.4 859,714 + PFLU_RS31400 0.36 +0.0 859,730 + PFLU_RS31400 0.44 +0.6 859,730 + PFLU_RS31400 0.44 +0.3 859,731 - PFLU_RS31400 0.45 -2.0 859,731 - PFLU_RS31400 0.45 -1.3 859,748 + PFLU_RS31400 0.54 -0.7 859,748 + PFLU_RS31400 0.54 -0.2 859,748 + PFLU_RS31400 0.54 -0.2 859,748 + PFLU_RS31400 0.54 -0.1 859,748 + PFLU_RS31400 0.54 -0.8 859,748 + PFLU_RS31400 0.54 -1.3 859,748 + PFLU_RS31400 0.54 -2.0 859,748 + PFLU_RS31400 0.54 +0.2 859,749 - PFLU_RS31400 0.55 -2.8 859,749 - PFLU_RS31400 0.55 -2.0 859,749 - PFLU_RS31400 0.55 -1.5 859,749 - PFLU_RS31400 0.55 -1.2 859,786 + PFLU_RS31400 0.75 -1.5 859,786 + PFLU_RS31400 0.75 -1.1 859,786 + PFLU_RS31400 0.75 -1.9 859,786 + PFLU_RS31400 0.75 -1.1 859,786 + PFLU_RS31400 0.75 -1.8 859,787 - PFLU_RS31400 0.75 -1.0 859,787 - PFLU_RS31400 0.75 -0.4 859,787 - PFLU_RS31400 0.75 -0.2 859,787 - PFLU_RS31400 0.75 -1.4 859,787 - PFLU_RS31400 0.75 -0.5 859,787 - PFLU_RS31400 0.75 -2.0 859,787 - PFLU_RS31400 0.75 -1.9 859,787 - PFLU_RS31400 0.75 -1.7 859,787 - PFLU_RS31400 0.75 -0.2 859,787 - PFLU_RS31400 0.75 +0.6 859,787 - PFLU_RS31400 0.75 -1.0 859,806 + PFLU_RS31400 0.86 -2.5 859,806 + PFLU_RS31400 0.86 +1.0 859,806 + PFLU_RS31400 0.86 -0.7 859,807 - PFLU_RS31400 0.86 -1.6 859,807 - PFLU_RS31400 0.86 -2.0 859,807 - PFLU_RS31400 0.86 -1.1 859,907 + PFLU_RS03765 0.10 -1.8 859,907 + PFLU_RS03765 0.10 -0.4 859,908 - PFLU_RS03765 0.11 +0.6 859,908 - PFLU_RS03765 0.11 -1.3 859,918 + PFLU_RS03765 0.12 -1.4 860,024 + PFLU_RS03765 0.23 -0.8 860,024 + PFLU_RS03765 0.23 -1.4 860,024 + PFLU_RS03765 0.23 -1.7 860,024 + PFLU_RS03765 0.23 +1.3 860,024 + PFLU_RS03765 0.23 -1.8 860,024 + PFLU_RS03765 0.23 -0.7 860,024 + PFLU_RS03765 0.23 -0.7 860,025 - PFLU_RS03765 0.23 -1.8 860,025 - PFLU_RS03765 0.23 -0.7 860,025 - PFLU_RS03765 0.23 +1.0 860,025 - PFLU_RS03765 0.23 -0.2 860,025 - PFLU_RS03765 0.23 -2.0 860,025 - PFLU_RS03765 0.23 -0.5 860,025 - PFLU_RS03765 0.23 -1.8 860,025 - PFLU_RS03765 0.23 -2.0 860,072 - PFLU_RS03765 0.28 -1.1 860,144 - PFLU_RS03765 0.36 +0.3 860,252 + PFLU_RS03765 0.48 -1.0 860,318 + PFLU_RS03765 0.55 +0.6 860,318 + PFLU_RS03765 0.55 -1.8 860,319 - PFLU_RS03765 0.55 -1.2 860,319 - PFLU_RS03765 0.55 -1.8 860,396 + PFLU_RS03765 0.64 -1.3 860,396 + PFLU_RS03765 0.64 +0.6 860,396 + PFLU_RS03765 0.64 -2.3 860,396 + PFLU_RS03765 0.64 +0.6 860,396 + PFLU_RS03765 0.64 -2.7 860,396 + PFLU_RS03765 0.64 -1.1 860,396 + PFLU_RS03765 0.64 -0.4 860,396 + PFLU_RS03765 0.64 -2.0 860,396 + PFLU_RS03765 0.64 -2.7 860,397 - PFLU_RS03765 0.64 -2.6 860,397 - PFLU_RS03765 0.64 -1.5 860,397 - PFLU_RS03765 0.64 +0.0 860,397 - PFLU_RS03765 0.64 -0.5 860,397 - PFLU_RS03765 0.64 -0.2 860,402 + PFLU_RS03765 0.64 +0.3 860,402 + PFLU_RS03765 0.64 -1.3 860,402 + PFLU_RS03765 0.64 -2.7 860,402 + PFLU_RS03765 0.64 -0.2 860,402 + PFLU_RS03765 0.64 -1.5 860,402 + PFLU_RS03765 0.64 -2.2 860,402 + PFLU_RS03765 0.64 -2.2 860,403 - PFLU_RS03765 0.64 -0.2 860,403 - PFLU_RS03765 0.64 -0.4 860,403 - PFLU_RS03765 0.64 -1.0 860,403 - PFLU_RS03765 0.64 -0.8 860,403 - PFLU_RS03765 0.64 -2.4 860,403 - PFLU_RS03765 0.64 -1.2 860,403 - PFLU_RS03765 0.64 -0.9 860,403 - PFLU_RS03765 0.64 -2.7 860,403 - PFLU_RS03765 0.64 -1.3 860,403 - PFLU_RS03765 0.64 -0.7 860,424 + PFLU_RS03765 0.67 -1.2 860,424 + PFLU_RS03765 0.67 -3.0 860,425 - PFLU_RS03765 0.67 -2.7 860,432 + PFLU_RS03765 0.68 -1.4 860,433 - PFLU_RS03765 0.68 -1.4 860,433 - PFLU_RS03765 0.68 -0.5 860,533 + PFLU_RS03765 0.79 +0.6 860,560 + PFLU_RS03765 0.82 -1.2 860,704 + -0.7 860,704 + -2.1 860,705 - -1.0 860,720 + -1.2 860,721 - +0.0 860,726 + +0.0 860,727 - -1.5 860,727 - -2.1 860,727 - +0.6 860,780 + -0.2 860,781 - -2.5
Or see this region's nucleotide sequence