Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS24445 and PFLU_RS24450 are separated by 50 nucleotides PFLU_RS24450 and PFLU_RS24455 are separated by 247 nucleotides PFLU_RS24455 and PFLU_RS24460 are separated by 66 nucleotides PFLU_RS24460 and PFLU_RS24465 are separated by 117 nucleotides
PFLU_RS24445: PFLU_RS24445 - chaperonin GroEL, at 5,477,601 to 5,479,247
_RS24445
PFLU_RS24450: PFLU_RS24450 - co-chaperone GroES, at 5,479,298 to 5,479,594
_RS24450
PFLU_RS24455: PFLU_RS24455 - membrane protein FxsA, at 5,479,842 to 5,480,318
_RS24455
PFLU_RS24460: PFLU_RS24460 - HugZ family protein, at 5,480,385 to 5,481,116
_RS24460
PFLU_RS24465: PFLU_RS24465 - DUF481 domain-containing protein, at 5,481,234 to 5,482,241
_RS24465
Position (kb) 5479
5480
5481 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5479.273 kb on - strand at 5479.273 kb on - strand at 5479.276 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.735 kb on - strand at 5479.739 kb on - strand at 5479.739 kb on - strand at 5479.739 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.749 kb on - strand at 5479.755 kb on - strand at 5479.755 kb on - strand at 5479.755 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.758 kb on - strand at 5479.843 kb on + strand at 5479.843 kb on + strand at 5479.844 kb on - strand at 5479.844 kb on - strand at 5480.006 kb on + strand, within PFLU_RS24455 at 5480.006 kb on + strand, within PFLU_RS24455 at 5480.007 kb on - strand, within PFLU_RS24455 at 5480.051 kb on + strand, within PFLU_RS24455 at 5480.052 kb on - strand, within PFLU_RS24455 at 5480.154 kb on + strand, within PFLU_RS24455 at 5480.154 kb on + strand, within PFLU_RS24455 at 5480.154 kb on + strand, within PFLU_RS24455 at 5480.154 kb on + strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.155 kb on - strand, within PFLU_RS24455 at 5480.265 kb on + strand, within PFLU_RS24455 at 5480.265 kb on + strand, within PFLU_RS24455 at 5480.266 kb on - strand, within PFLU_RS24455 at 5480.304 kb on + strand at 5480.304 kb on + strand at 5480.304 kb on + strand at 5480.305 kb on - strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.360 kb on + strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.361 kb on - strand at 5480.510 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.522 kb on + strand, within PFLU_RS24460 at 5480.523 kb on - strand, within PFLU_RS24460 at 5480.523 kb on - strand, within PFLU_RS24460 at 5480.523 kb on - strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.587 kb on + strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.588 kb on - strand, within PFLU_RS24460 at 5480.713 kb on + strand, within PFLU_RS24460 at 5480.714 kb on - strand, within PFLU_RS24460 at 5480.714 kb on - strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.767 kb on + strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.768 kb on - strand, within PFLU_RS24460 at 5480.788 kb on + strand, within PFLU_RS24460 at 5480.788 kb on + strand, within PFLU_RS24460 at 5480.788 kb on + strand, within PFLU_RS24460 at 5480.788 kb on + strand, within PFLU_RS24460 at 5480.788 kb on + strand, within PFLU_RS24460 at 5480.789 kb on - strand, within PFLU_RS24460 at 5480.789 kb on - strand, within PFLU_RS24460 at 5480.789 kb on - strand, within PFLU_RS24460 at 5480.789 kb on - strand, within PFLU_RS24460 at 5480.789 kb on - strand, within PFLU_RS24460 at 5480.795 kb on - strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.797 kb on + strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.798 kb on - strand, within PFLU_RS24460 at 5480.857 kb on + strand, within PFLU_RS24460 at 5480.857 kb on + strand, within PFLU_RS24460 at 5480.857 kb on + strand, within PFLU_RS24460 at 5480.857 kb on + strand, within PFLU_RS24460 at 5480.857 kb on + strand, within PFLU_RS24460 at 5480.858 kb on - strand, within PFLU_RS24460 at 5480.858 kb on - strand, within PFLU_RS24460 at 5480.858 kb on - strand, within PFLU_RS24460 at 5480.858 kb on - strand, within PFLU_RS24460 at 5480.858 kb on - strand, within PFLU_RS24460 at 5481.002 kb on - strand, within PFLU_RS24460 at 5481.002 kb on - strand, within PFLU_RS24460 at 5481.067 kb on + strand at 5481.068 kb on - strand at 5481.068 kb on - strand at 5481.152 kb on + strand at 5481.153 kb on - strand at 5481.177 kb on + strand at 5481.177 kb on + strand at 5481.177 kb on + strand at 5481.177 kb on + strand at 5481.177 kb on + strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.178 kb on - strand at 5481.186 kb on + strand at 5481.186 kb on + strand at 5481.186 kb on + strand at 5481.186 kb on + strand at 5481.186 kb on + strand at 5481.186 kb on + strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.187 kb on - strand at 5481.235 kb on + strand at 5481.236 kb on - strand at 5481.262 kb on + strand at 5481.262 kb on + strand at 5481.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 5,479,273 - -0.2 5,479,273 - +0.8 5,479,276 - +0.0 5,479,735 - +3.9 5,479,735 - +2.0 5,479,735 - +2.5 5,479,735 - -1.0 5,479,735 - +1.7 5,479,735 - -1.6 5,479,735 - -1.3 5,479,739 - -0.5 5,479,739 - +0.0 5,479,739 - -1.0 5,479,749 - +0.4 5,479,749 - +0.0 5,479,749 - +0.9 5,479,749 - -0.6 5,479,749 - -0.6 5,479,749 - -0.5 5,479,749 - -1.1 5,479,749 - -2.6 5,479,755 - +3.2 5,479,755 - -0.6 5,479,755 - +0.6 5,479,758 - -0.3 5,479,758 - -1.2 5,479,758 - -0.5 5,479,758 - -0.6 5,479,758 - -2.0 5,479,758 - +0.4 5,479,758 - +0.9 5,479,758 - -1.3 5,479,758 - +1.8 5,479,758 - -0.2 5,479,758 - -2.4 5,479,758 - -0.5 5,479,758 - +0.1 5,479,758 - -0.8 5,479,758 - -1.6 5,479,843 + -0.5 5,479,843 + +0.9 5,479,844 - -1.6 5,479,844 - -1.6 5,480,006 + PFLU_RS24455 0.34 -1.0 5,480,006 + PFLU_RS24455 0.34 +1.8 5,480,007 - PFLU_RS24455 0.35 +1.4 5,480,051 + PFLU_RS24455 0.44 -0.8 5,480,052 - PFLU_RS24455 0.44 +0.5 5,480,154 + PFLU_RS24455 0.65 +0.0 5,480,154 + PFLU_RS24455 0.65 +1.9 5,480,154 + PFLU_RS24455 0.65 -1.0 5,480,154 + PFLU_RS24455 0.65 -0.5 5,480,155 - PFLU_RS24455 0.66 -2.2 5,480,155 - PFLU_RS24455 0.66 +0.5 5,480,155 - PFLU_RS24455 0.66 +0.0 5,480,155 - PFLU_RS24455 0.66 -2.0 5,480,155 - PFLU_RS24455 0.66 -1.6 5,480,155 - PFLU_RS24455 0.66 -0.6 5,480,265 + PFLU_RS24455 0.89 +0.0 5,480,265 + PFLU_RS24455 0.89 -1.6 5,480,266 - PFLU_RS24455 0.89 -2.7 5,480,304 + +3.6 5,480,304 + +0.0 5,480,304 + -0.6 5,480,305 - -1.5 5,480,360 + +2.1 5,480,360 + +0.4 5,480,360 + -1.6 5,480,360 + -1.0 5,480,360 + -0.5 5,480,360 + +0.4 5,480,360 + -1.6 5,480,360 + +0.8 5,480,360 + +0.4 5,480,360 + -2.6 5,480,360 + -1.3 5,480,360 + -1.5 5,480,360 + -0.1 5,480,361 - -1.2 5,480,361 - -0.9 5,480,361 - +2.0 5,480,361 - +0.3 5,480,361 - +1.9 5,480,361 - +3.1 5,480,361 - -0.5 5,480,361 - -1.0 5,480,361 - +2.0 5,480,361 - -1.7 5,480,361 - -0.8 5,480,361 - -1.5 5,480,361 - +0.9 5,480,361 - +0.0 5,480,361 - -1.3 5,480,361 - -2.2 5,480,361 - -2.0 5,480,361 - -2.0 5,480,361 - -2.5 5,480,361 - -0.7 5,480,361 - +0.0 5,480,510 + PFLU_RS24460 0.17 -0.8 5,480,522 + PFLU_RS24460 0.19 -2.6 5,480,522 + PFLU_RS24460 0.19 -1.1 5,480,522 + PFLU_RS24460 0.19 +0.8 5,480,522 + PFLU_RS24460 0.19 +0.6 5,480,522 + PFLU_RS24460 0.19 -0.6 5,480,522 + PFLU_RS24460 0.19 -0.1 5,480,522 + PFLU_RS24460 0.19 -0.2 5,480,523 - PFLU_RS24460 0.19 -1.2 5,480,523 - PFLU_RS24460 0.19 -2.0 5,480,523 - PFLU_RS24460 0.19 -0.8 5,480,587 + PFLU_RS24460 0.28 +3.6 5,480,587 + PFLU_RS24460 0.28 +2.1 5,480,587 + PFLU_RS24460 0.28 -0.3 5,480,587 + PFLU_RS24460 0.28 +1.7 5,480,587 + PFLU_RS24460 0.28 -0.0 5,480,587 + PFLU_RS24460 0.28 -2.3 5,480,587 + PFLU_RS24460 0.28 -0.6 5,480,587 + PFLU_RS24460 0.28 -0.9 5,480,588 - PFLU_RS24460 0.28 -1.2 5,480,588 - PFLU_RS24460 0.28 +2.1 5,480,588 - PFLU_RS24460 0.28 +0.4 5,480,588 - PFLU_RS24460 0.28 +1.4 5,480,588 - PFLU_RS24460 0.28 +1.4 5,480,588 - PFLU_RS24460 0.28 -0.2 5,480,713 + PFLU_RS24460 0.45 -0.5 5,480,714 - PFLU_RS24460 0.45 -0.2 5,480,714 - PFLU_RS24460 0.45 +1.5 5,480,767 + PFLU_RS24460 0.52 +0.8 5,480,767 + PFLU_RS24460 0.52 +1.0 5,480,767 + PFLU_RS24460 0.52 +0.4 5,480,767 + PFLU_RS24460 0.52 -0.8 5,480,767 + PFLU_RS24460 0.52 +3.9 5,480,767 + PFLU_RS24460 0.52 -1.1 5,480,767 + PFLU_RS24460 0.52 -0.5 5,480,767 + PFLU_RS24460 0.52 -0.1 5,480,767 + PFLU_RS24460 0.52 +1.2 5,480,767 + PFLU_RS24460 0.52 +3.2 5,480,767 + PFLU_RS24460 0.52 +0.4 5,480,768 - PFLU_RS24460 0.52 -0.2 5,480,768 - PFLU_RS24460 0.52 -2.5 5,480,768 - PFLU_RS24460 0.52 +0.2 5,480,768 - PFLU_RS24460 0.52 -0.3 5,480,768 - PFLU_RS24460 0.52 +2.7 5,480,768 - PFLU_RS24460 0.52 -1.0 5,480,768 - PFLU_RS24460 0.52 +1.8 5,480,768 - PFLU_RS24460 0.52 +2.5 5,480,788 + PFLU_RS24460 0.55 -2.0 5,480,788 + PFLU_RS24460 0.55 +0.5 5,480,788 + PFLU_RS24460 0.55 -1.3 5,480,788 + PFLU_RS24460 0.55 +2.8 5,480,788 + PFLU_RS24460 0.55 +3.6 5,480,789 - PFLU_RS24460 0.55 -2.0 5,480,789 - PFLU_RS24460 0.55 -0.5 5,480,789 - PFLU_RS24460 0.55 +1.5 5,480,789 - PFLU_RS24460 0.55 -1.0 5,480,789 - PFLU_RS24460 0.55 -1.6 5,480,795 - PFLU_RS24460 0.56 +2.6 5,480,797 + PFLU_RS24460 0.56 +3.0 5,480,797 + PFLU_RS24460 0.56 +2.1 5,480,797 + PFLU_RS24460 0.56 -0.8 5,480,797 + PFLU_RS24460 0.56 +2.8 5,480,797 + PFLU_RS24460 0.56 +1.7 5,480,797 + PFLU_RS24460 0.56 -3.3 5,480,798 - PFLU_RS24460 0.56 -2.0 5,480,798 - PFLU_RS24460 0.56 -2.0 5,480,798 - PFLU_RS24460 0.56 -1.0 5,480,798 - PFLU_RS24460 0.56 +0.2 5,480,798 - PFLU_RS24460 0.56 +0.0 5,480,798 - PFLU_RS24460 0.56 +0.9 5,480,857 + PFLU_RS24460 0.64 +0.0 5,480,857 + PFLU_RS24460 0.64 -1.2 5,480,857 + PFLU_RS24460 0.64 +1.4 5,480,857 + PFLU_RS24460 0.64 -0.4 5,480,857 + PFLU_RS24460 0.64 -0.2 5,480,858 - PFLU_RS24460 0.65 +0.1 5,480,858 - PFLU_RS24460 0.65 -0.8 5,480,858 - PFLU_RS24460 0.65 +4.3 5,480,858 - PFLU_RS24460 0.65 -1.9 5,480,858 - PFLU_RS24460 0.65 +1.2 5,481,002 - PFLU_RS24460 0.84 -0.7 5,481,002 - PFLU_RS24460 0.84 -1.6 5,481,067 + +1.6 5,481,068 - +3.5 5,481,068 - +0.4 5,481,152 + +0.0 5,481,153 - -0.6 5,481,177 + +1.2 5,481,177 + +1.9 5,481,177 + +0.0 5,481,177 + -0.2 5,481,177 + -2.0 5,481,178 - +2.0 5,481,178 - -2.2 5,481,178 - +2.2 5,481,178 - +0.0 5,481,178 - +0.9 5,481,178 - +2.1 5,481,178 - -3.1 5,481,186 + -1.5 5,481,186 + +0.8 5,481,186 + -2.0 5,481,186 + -2.8 5,481,186 + +0.7 5,481,186 + +1.6 5,481,187 - -0.8 5,481,187 - -1.5 5,481,187 - +1.9 5,481,187 - -0.5 5,481,187 - +1.9 5,481,187 - +0.4 5,481,187 - -0.1 5,481,187 - -2.5 5,481,235 + +0.1 5,481,236 - +1.0 5,481,262 + -1.6 5,481,262 + +1.6 5,481,263 - +0.8
Or see this region's nucleotide sequence