Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03010 and PFLU_RS03015 are separated by 168 nucleotides PFLU_RS03015 and PFLU_RS03020 are separated by 81 nucleotides PFLU_RS03020 and PFLU_RS03025 overlap by 4 nucleotides
PFLU_RS03010: PFLU_RS03010 - phosphoribosylamine--glycine ligase, at 693,697 to 694,989
_RS03010
PFLU_RS03015: PFLU_RS03015 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 695,158 to 696,765
_RS03015
PFLU_RS03020: PFLU_RS03020 - DNA-binding transcriptional regulator Fis, at 696,847 to 697,167
_RS03020
PFLU_RS03025: PFLU_RS03025 - tRNA dihydrouridine synthase DusB, at 697,164 to 698,177
_RS03025
Position (kb) 695
696
697 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 694.182 kb on - strand, within PFLU_RS03010 at 695.011 kb on - strand at 695.011 kb on - strand at 695.030 kb on + strand at 695.030 kb on + strand at 695.030 kb on + strand at 695.030 kb on + strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.031 kb on - strand at 695.132 kb on + strand at 695.132 kb on + strand at 695.133 kb on - strand at 695.133 kb on - strand at 695.613 kb on - strand, within PFLU_RS03015 at 695.613 kb on - strand, within PFLU_RS03015 at 696.186 kb on - strand, within PFLU_RS03015 at 696.815 kb on + strand at 696.848 kb on + strand at 696.848 kb on + strand at 696.848 kb on + strand at 696.848 kb on + strand at 696.849 kb on - strand at 696.849 kb on - strand at 696.849 kb on - strand at 696.849 kb on - strand at 697.207 kb on + strand at 697.208 kb on - strand at 697.208 kb on - strand at 697.208 kb on - strand at 697.208 kb on - strand at 697.208 kb on - strand at 697.341 kb on + strand, within PFLU_RS03025 at 697.342 kb on - strand, within PFLU_RS03025 at 697.342 kb on - strand, within PFLU_RS03025 at 697.342 kb on - strand, within PFLU_RS03025 at 697.360 kb on + strand, within PFLU_RS03025 at 697.361 kb on - strand, within PFLU_RS03025 at 697.361 kb on - strand, within PFLU_RS03025 at 697.361 kb on - strand, within PFLU_RS03025 at 697.397 kb on + strand, within PFLU_RS03025 at 697.398 kb on - strand, within PFLU_RS03025 at 697.398 kb on - strand, within PFLU_RS03025 at 697.398 kb on - strand, within PFLU_RS03025 at 697.398 kb on - strand, within PFLU_RS03025 at 697.432 kb on - strand, within PFLU_RS03025 at 697.432 kb on - strand, within PFLU_RS03025 at 697.432 kb on - strand, within PFLU_RS03025 at 697.621 kb on + strand, within PFLU_RS03025 at 697.622 kb on - strand, within PFLU_RS03025 at 697.640 kb on - strand, within PFLU_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 694,182 - PFLU_RS03010 0.38 +0.1 695,011 - -1.1 695,011 - +1.1 695,030 + +1.2 695,030 + -0.5 695,030 + +1.2 695,030 + -1.9 695,031 - -1.2 695,031 - +1.5 695,031 - -2.3 695,031 - -1.6 695,031 - -2.0 695,031 - -0.1 695,031 - -2.6 695,132 + +2.1 695,132 + +1.7 695,133 - +0.2 695,133 - +1.9 695,613 - PFLU_RS03015 0.28 +0.4 695,613 - PFLU_RS03015 0.28 +1.1 696,186 - PFLU_RS03015 0.64 +1.1 696,815 + +1.7 696,848 + -1.7 696,848 + +0.9 696,848 + +2.2 696,848 + -0.5 696,849 - -1.4 696,849 - -1.6 696,849 - -0.8 696,849 - -1.9 697,207 + +0.7 697,208 - +1.5 697,208 - +1.9 697,208 - +0.6 697,208 - +0.2 697,208 - -1.6 697,341 + PFLU_RS03025 0.17 +0.1 697,342 - PFLU_RS03025 0.18 -0.6 697,342 - PFLU_RS03025 0.18 +3.8 697,342 - PFLU_RS03025 0.18 -0.4 697,360 + PFLU_RS03025 0.19 +0.4 697,361 - PFLU_RS03025 0.19 +2.1 697,361 - PFLU_RS03025 0.19 -0.8 697,361 - PFLU_RS03025 0.19 +2.8 697,397 + PFLU_RS03025 0.23 +1.7 697,398 - PFLU_RS03025 0.23 -1.8 697,398 - PFLU_RS03025 0.23 -2.2 697,398 - PFLU_RS03025 0.23 +1.4 697,398 - PFLU_RS03025 0.23 +2.7 697,432 - PFLU_RS03025 0.26 +2.6 697,432 - PFLU_RS03025 0.26 +4.7 697,432 - PFLU_RS03025 0.26 +2.0 697,621 + PFLU_RS03025 0.45 -0.1 697,622 - PFLU_RS03025 0.45 +2.6 697,640 - PFLU_RS03025 0.47 +2.5
Or see this region's nucleotide sequence