Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08125 and MMJJ_RS08130 are separated by 36 nucleotides MMJJ_RS08130 and MMJJ_RS08135 are separated by 161 nucleotides MMJJ_RS08135 and MMJJ_RS08140 are separated by 210 nucleotides
MMJJ_RS08125: MMJJ_RS08125 - DNA double-strand break repair nuclease NurA, at 1,490,846 to 1,491,910
_RS08125
MMJJ_RS08130: MMJJ_RS08130 - translation initiation factor IF-2 subunit gamma, at 1,491,947 to 1,493,179
_RS08130
MMJJ_RS08135: MMJJ_RS08135 - 30S ribosomal protein S6e, at 1,493,341 to 1,493,715
_RS08135
MMJJ_RS08140: MMJJ_RS08140 - type I glutamate--ammonia ligase, at 1,493,926 to 1,495,266
_RS08140
Position (kb)
1491
1492
1493
1494 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1490.961 kb on - strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.962 kb on + strand, within MMJJ_RS08125 at 1490.982 kb on + strand, within MMJJ_RS08125 at 1491.114 kb on - strand, within MMJJ_RS08125 at 1491.115 kb on + strand, within MMJJ_RS08125 at 1491.238 kb on + strand, within MMJJ_RS08125 at 1491.241 kb on - strand, within MMJJ_RS08125 at 1491.305 kb on - strand, within MMJJ_RS08125 at 1491.306 kb on + strand, within MMJJ_RS08125 at 1491.309 kb on + strand, within MMJJ_RS08125 at 1491.309 kb on + strand, within MMJJ_RS08125 at 1491.311 kb on - strand, within MMJJ_RS08125 at 1491.311 kb on - strand, within MMJJ_RS08125 at 1491.453 kb on - strand, within MMJJ_RS08125 at 1491.454 kb on + strand, within MMJJ_RS08125 at 1491.454 kb on + strand, within MMJJ_RS08125 at 1491.464 kb on + strand, within MMJJ_RS08125 at 1491.467 kb on + strand, within MMJJ_RS08125 at 1491.488 kb on + strand, within MMJJ_RS08125 at 1491.525 kb on - strand, within MMJJ_RS08125 at 1491.525 kb on - strand, within MMJJ_RS08125 at 1491.526 kb on + strand, within MMJJ_RS08125 at 1491.526 kb on + strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.527 kb on - strand, within MMJJ_RS08125 at 1491.528 kb on + strand, within MMJJ_RS08125 at 1491.528 kb on + strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.546 kb on - strand, within MMJJ_RS08125 at 1491.547 kb on + strand, within MMJJ_RS08125 at 1491.550 kb on + strand, within MMJJ_RS08125 at 1491.633 kb on - strand, within MMJJ_RS08125 at 1491.684 kb on + strand, within MMJJ_RS08125 at 1491.708 kb on - strand, within MMJJ_RS08125 at 1491.708 kb on - strand, within MMJJ_RS08125 at 1491.738 kb on - strand, within MMJJ_RS08125 at 1491.744 kb on + strand, within MMJJ_RS08125 at 1491.744 kb on + strand, within MMJJ_RS08125 at 1491.746 kb on + strand, within MMJJ_RS08125 at 1491.793 kb on - strand, within MMJJ_RS08125 at 1491.806 kb on + strand at 1491.832 kb on - strand at 1491.833 kb on + strand at 1491.866 kb on + strand at 1491.872 kb on - strand at 1491.872 kb on - strand at 1491.916 kb on + strand at 1491.928 kb on - strand at 1491.953 kb on - strand at 1491.954 kb on + strand at 1492.249 kb on - strand at 1492.545 kb on + strand, within MMJJ_RS08130 at 1493.214 kb on - strand at 1493.283 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.285 kb on - strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.286 kb on + strand at 1493.289 kb on - strand at 1493.289 kb on - strand at 1493.289 kb on - strand at 1493.290 kb on + strand at 1493.290 kb on + strand at 1493.291 kb on - strand at 1493.291 kb on - strand at 1493.292 kb on + strand at 1493.292 kb on + strand at 1493.292 kb on + strand at 1493.319 kb on + strand at 1493.337 kb on - strand at 1493.407 kb on - strand, within MMJJ_RS08135 at 1493.412 kb on - strand, within MMJJ_RS08135 at 1493.412 kb on - strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.413 kb on + strand, within MMJJ_RS08135 at 1493.458 kb on + strand, within MMJJ_RS08135 at 1493.503 kb on - strand, within MMJJ_RS08135 at 1493.504 kb on + strand, within MMJJ_RS08135 at 1493.593 kb on - strand, within MMJJ_RS08135 at 1493.718 kb on - strand at 1493.721 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.742 kb on - strand at 1493.743 kb on + strand at 1493.802 kb on - strand at 1493.821 kb on - strand at 1493.822 kb on + strand at 1493.828 kb on + strand at 1493.828 kb on + strand at 1493.832 kb on - strand at 1493.833 kb on + strand at 1493.836 kb on + strand at 1493.898 kb on - strand at 1493.899 kb on + strand at 1493.899 kb on + strand at 1493.914 kb on + strand at 1493.924 kb on + strand at 1493.928 kb on + strand at 1493.936 kb on + strand at 1493.936 kb on + strand at 1493.936 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 1,490,961 - MMJJ_RS08125 0.11 -0.2 1,490,962 + MMJJ_RS08125 0.11 +0.6 1,490,962 + MMJJ_RS08125 0.11 -1.7 1,490,962 + MMJJ_RS08125 0.11 +0.2 1,490,982 + MMJJ_RS08125 0.13 -1.5 1,491,114 - MMJJ_RS08125 0.25 +0.5 1,491,115 + MMJJ_RS08125 0.25 +0.4 1,491,238 + MMJJ_RS08125 0.37 -0.2 1,491,241 - MMJJ_RS08125 0.37 -1.1 1,491,305 - MMJJ_RS08125 0.43 +0.0 1,491,306 + MMJJ_RS08125 0.43 +1.1 1,491,309 + MMJJ_RS08125 0.43 +0.1 1,491,309 + MMJJ_RS08125 0.43 +0.6 1,491,311 - MMJJ_RS08125 0.44 -0.5 1,491,311 - MMJJ_RS08125 0.44 +0.3 1,491,453 - MMJJ_RS08125 0.57 -0.1 1,491,454 + MMJJ_RS08125 0.57 -0.5 1,491,454 + MMJJ_RS08125 0.57 +0.1 1,491,464 + MMJJ_RS08125 0.58 -1.6 1,491,467 + MMJJ_RS08125 0.58 +0.4 1,491,488 + MMJJ_RS08125 0.60 -0.8 1,491,525 - MMJJ_RS08125 0.64 -0.8 1,491,525 - MMJJ_RS08125 0.64 -0.1 1,491,526 + MMJJ_RS08125 0.64 -0.4 1,491,526 + MMJJ_RS08125 0.64 -0.3 1,491,527 - MMJJ_RS08125 0.64 -0.8 1,491,527 - MMJJ_RS08125 0.64 +0.5 1,491,527 - MMJJ_RS08125 0.64 -1.2 1,491,528 + MMJJ_RS08125 0.64 -0.4 1,491,528 + MMJJ_RS08125 0.64 -1.0 1,491,546 - MMJJ_RS08125 0.66 +0.4 1,491,546 - MMJJ_RS08125 0.66 -0.4 1,491,546 - MMJJ_RS08125 0.66 -0.9 1,491,547 + MMJJ_RS08125 0.66 -0.2 1,491,550 + MMJJ_RS08125 0.66 -0.0 1,491,633 - MMJJ_RS08125 0.74 +1.1 1,491,684 + MMJJ_RS08125 0.79 -0.4 1,491,708 - MMJJ_RS08125 0.81 +0.3 1,491,708 - MMJJ_RS08125 0.81 -0.5 1,491,738 - MMJJ_RS08125 0.84 +0.9 1,491,744 + MMJJ_RS08125 0.84 -0.8 1,491,744 + MMJJ_RS08125 0.84 +0.1 1,491,746 + MMJJ_RS08125 0.85 -3.8 1,491,793 - MMJJ_RS08125 0.89 -0.2 1,491,806 + -1.0 1,491,832 - -0.1 1,491,833 + -1.1 1,491,866 + +0.8 1,491,872 - +0.2 1,491,872 - +0.8 1,491,916 + -0.7 1,491,928 - -1.0 1,491,953 - -0.4 1,491,954 + -0.4 1,492,249 - +0.1 1,492,545 + MMJJ_RS08130 0.48 -2.0 1,493,214 - -1.2 1,493,283 - -1.9 1,493,285 - -0.9 1,493,285 - -1.8 1,493,285 - -3.3 1,493,285 - -1.3 1,493,285 - -1.5 1,493,286 + -1.1 1,493,286 + -2.6 1,493,286 + +0.1 1,493,286 + -1.7 1,493,286 + -2.3 1,493,286 + -4.2 1,493,286 + -1.4 1,493,286 + -1.6 1,493,289 - +0.6 1,493,289 - +0.8 1,493,289 - +0.4 1,493,290 + +0.5 1,493,290 + +0.4 1,493,291 - -2.8 1,493,291 - -0.7 1,493,292 + -0.9 1,493,292 + -0.1 1,493,292 + +0.2 1,493,319 + -0.3 1,493,337 - +0.3 1,493,407 - MMJJ_RS08135 0.18 -0.7 1,493,412 - MMJJ_RS08135 0.19 +0.2 1,493,412 - MMJJ_RS08135 0.19 +0.0 1,493,413 + MMJJ_RS08135 0.19 -1.9 1,493,413 + MMJJ_RS08135 0.19 +0.9 1,493,413 + MMJJ_RS08135 0.19 +0.4 1,493,458 + MMJJ_RS08135 0.31 -0.1 1,493,503 - MMJJ_RS08135 0.43 -1.3 1,493,504 + MMJJ_RS08135 0.43 +0.0 1,493,593 - MMJJ_RS08135 0.67 -0.8 1,493,718 - -0.6 1,493,721 - -0.5 1,493,742 - -0.0 1,493,742 - -1.7 1,493,742 - +0.7 1,493,742 - -0.1 1,493,743 + -1.5 1,493,802 - -0.6 1,493,821 - -1.4 1,493,822 + +0.0 1,493,828 + -0.6 1,493,828 + -1.3 1,493,832 - -0.1 1,493,833 + -0.7 1,493,836 + -0.6 1,493,898 - +2.6 1,493,899 + -1.6 1,493,899 + +0.1 1,493,914 + +0.7 1,493,924 + -0.4 1,493,928 + -0.3 1,493,936 + -1.7 1,493,936 + +0.1 1,493,936 + +0.3
Or see this region's nucleotide sequence