Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05070 and MMJJ_RS05075 are separated by 67 nucleotides MMJJ_RS05075 and MMJJ_RS05080 are separated by 259 nucleotides MMJJ_RS05080 and MMJJ_RS05085 are separated by 44 nucleotides MMJJ_RS05085 and MMJJ_RS05090 are separated by 253 nucleotides MMJJ_RS05090 and MMJJ_RS05095 are separated by 59 nucleotides
MMJJ_RS05070: MMJJ_RS05070 - DNA primase catalytic subunit PriS, at 941,927 to 943,000
_RS05070
MMJJ_RS05075: MMJJ_RS05075 - tRNA-Gln, at 943,068 to 943,143
_RS05075
MMJJ_RS05080: MMJJ_RS05080 - tRNA-Thr, at 943,403 to 943,476
_RS05080
MMJJ_RS05085: MMJJ_RS05085 - hypothetical protein, at 943,521 to 944,459
_RS05085
MMJJ_RS05090: MMJJ_RS05090 - MGMT family protein, at 944,713 to 945,012
_RS05090
MMJJ_RS05095: MMJJ_RS05095 - ammonium transporter, at 945,072 to 946,307
_RS05095
Position (kb) 943
944
945 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 943.030 kb on + strand at 943.062 kb on - strand at 943.180 kb on - strand at 943.264 kb on - strand at 943.265 kb on + strand at 943.265 kb on + strand at 943.271 kb on - strand at 943.334 kb on + strand at 943.485 kb on + strand at 943.563 kb on - strand at 943.563 kb on - strand at 943.564 kb on + strand at 943.564 kb on + strand at 943.572 kb on + strand at 943.689 kb on - strand, within MMJJ_RS05085 at 943.690 kb on + strand, within MMJJ_RS05085 at 943.702 kb on + strand, within MMJJ_RS05085 at 943.741 kb on + strand, within MMJJ_RS05085 at 943.742 kb on - strand, within MMJJ_RS05085 at 943.743 kb on + strand, within MMJJ_RS05085 at 943.905 kb on - strand, within MMJJ_RS05085 at 943.906 kb on + strand, within MMJJ_RS05085 at 943.953 kb on - strand, within MMJJ_RS05085 at 943.974 kb on + strand, within MMJJ_RS05085 at 943.984 kb on + strand, within MMJJ_RS05085 at 944.001 kb on + strand, within MMJJ_RS05085 at 944.002 kb on - strand, within MMJJ_RS05085 at 944.002 kb on - strand, within MMJJ_RS05085 at 944.003 kb on + strand, within MMJJ_RS05085 at 944.010 kb on + strand, within MMJJ_RS05085 at 944.040 kb on - strand, within MMJJ_RS05085 at 944.040 kb on - strand, within MMJJ_RS05085 at 944.040 kb on - strand, within MMJJ_RS05085 at 944.042 kb on - strand, within MMJJ_RS05085 at 944.042 kb on - strand, within MMJJ_RS05085 at 944.062 kb on + strand, within MMJJ_RS05085 at 944.080 kb on + strand, within MMJJ_RS05085 at 944.238 kb on + strand, within MMJJ_RS05085 at 944.239 kb on - strand, within MMJJ_RS05085 at 944.245 kb on + strand, within MMJJ_RS05085 at 944.301 kb on - strand, within MMJJ_RS05085 at 944.301 kb on - strand, within MMJJ_RS05085 at 944.336 kb on - strand, within MMJJ_RS05085 at 944.336 kb on - strand, within MMJJ_RS05085 at 944.345 kb on + strand, within MMJJ_RS05085 at 944.349 kb on + strand, within MMJJ_RS05085 at 944.379 kb on - strand at 944.382 kb on - strand at 944.384 kb on - strand at 944.385 kb on + strand at 944.395 kb on + strand at 944.409 kb on - strand at 944.413 kb on - strand at 944.482 kb on - strand at 944.483 kb on + strand at 944.487 kb on + strand at 944.487 kb on + strand at 944.487 kb on + strand at 944.488 kb on - strand at 944.500 kb on - strand at 944.501 kb on + strand at 944.501 kb on + strand at 944.539 kb on + strand at 944.541 kb on - strand at 944.571 kb on + strand at 944.571 kb on + strand at 944.571 kb on + strand at 944.572 kb on - strand at 944.573 kb on + strand at 944.573 kb on + strand at 944.573 kb on + strand at 944.574 kb on - strand at 944.575 kb on + strand at 944.577 kb on - strand at 944.578 kb on + strand at 944.585 kb on - strand at 944.624 kb on + strand at 944.658 kb on + strand at 944.708 kb on - strand at 944.709 kb on + strand at 944.715 kb on + strand at 944.730 kb on + strand at 944.792 kb on + strand, within MMJJ_RS05090 at 944.843 kb on + strand, within MMJJ_RS05090 at 944.907 kb on - strand, within MMJJ_RS05090 at 944.907 kb on - strand at 944.916 kb on - strand, within MMJJ_RS05090 at 944.917 kb on + strand, within MMJJ_RS05090 at 944.920 kb on + strand, within MMJJ_RS05090 at 944.923 kb on - strand, within MMJJ_RS05090 at 944.938 kb on - strand, within MMJJ_RS05090 at 944.939 kb on + strand, within MMJJ_RS05090 at 944.945 kb on - strand, within MMJJ_RS05090 at 944.945 kb on - strand, within MMJJ_RS05090 at 944.945 kb on - strand, within MMJJ_RS05090 at 944.978 kb on + strand, within MMJJ_RS05090 at 944.979 kb on - strand, within MMJJ_RS05090 at 945.035 kb on - strand at 945.035 kb on - strand at 945.036 kb on + strand at 945.037 kb on - strand at 945.037 kb on - strand at 945.037 kb on - strand at 945.037 kb on - strand at 945.038 kb on + strand at 945.059 kb on - strand at 945.060 kb on + strand at 945.060 kb on + strand at 945.066 kb on + strand at 945.066 kb on + strand at 945.070 kb on + strand at 945.209 kb on + strand, within MMJJ_RS05095 at 945.235 kb on + strand, within MMJJ_RS05095 at 945.288 kb on - strand, within MMJJ_RS05095 at 945.330 kb on - strand, within MMJJ_RS05095 at 945.331 kb on + strand, within MMJJ_RS05095 at 945.340 kb on + strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.348 kb on - strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.349 kb on + strand, within MMJJ_RS05095 at 945.387 kb on - strand, within MMJJ_RS05095 at 945.388 kb on + strand, within MMJJ_RS05095 at 945.394 kb on + strand, within MMJJ_RS05095 at 945.420 kb on - strand, within MMJJ_RS05095 at 945.441 kb on - strand, within MMJJ_RS05095 at 945.441 kb on - strand, within MMJJ_RS05095
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 943,030 + +0.6 943,062 - +0.6 943,180 - -1.3 943,264 - +0.7 943,265 + +0.7 943,265 + -0.9 943,271 - -1.7 943,334 + -0.3 943,485 + -0.3 943,563 - +0.4 943,563 - +0.7 943,564 + +0.0 943,564 + +1.1 943,572 + -0.1 943,689 - MMJJ_RS05085 0.18 +0.8 943,690 + MMJJ_RS05085 0.18 -0.2 943,702 + MMJJ_RS05085 0.19 +0.6 943,741 + MMJJ_RS05085 0.23 -0.5 943,742 - MMJJ_RS05085 0.24 -1.7 943,743 + MMJJ_RS05085 0.24 -0.3 943,905 - MMJJ_RS05085 0.41 +0.0 943,906 + MMJJ_RS05085 0.41 -0.9 943,953 - MMJJ_RS05085 0.46 -0.5 943,974 + MMJJ_RS05085 0.48 +1.2 943,984 + MMJJ_RS05085 0.49 -0.7 944,001 + MMJJ_RS05085 0.51 +1.3 944,002 - MMJJ_RS05085 0.51 +0.7 944,002 - MMJJ_RS05085 0.51 +1.0 944,003 + MMJJ_RS05085 0.51 +0.1 944,010 + MMJJ_RS05085 0.52 +0.4 944,040 - MMJJ_RS05085 0.55 +0.7 944,040 - MMJJ_RS05085 0.55 +1.0 944,040 - MMJJ_RS05085 0.55 +0.5 944,042 - MMJJ_RS05085 0.55 +1.0 944,042 - MMJJ_RS05085 0.55 -0.5 944,062 + MMJJ_RS05085 0.58 +0.8 944,080 + MMJJ_RS05085 0.60 +0.1 944,238 + MMJJ_RS05085 0.76 +0.7 944,239 - MMJJ_RS05085 0.76 -0.5 944,245 + MMJJ_RS05085 0.77 +0.1 944,301 - MMJJ_RS05085 0.83 +0.5 944,301 - MMJJ_RS05085 0.83 +0.1 944,336 - MMJJ_RS05085 0.87 +0.1 944,336 - MMJJ_RS05085 0.87 -0.9 944,345 + MMJJ_RS05085 0.88 +0.1 944,349 + MMJJ_RS05085 0.88 +0.2 944,379 - -0.1 944,382 - -1.7 944,384 - +0.6 944,385 + -1.0 944,395 + +0.1 944,409 - +0.3 944,413 - -0.1 944,482 - +0.6 944,483 + +0.4 944,487 + -0.3 944,487 + +0.8 944,487 + +0.6 944,488 - -0.9 944,500 - +1.2 944,501 + -0.6 944,501 + +0.1 944,539 + +1.2 944,541 - -0.6 944,571 + +0.1 944,571 + -0.3 944,571 + -3.4 944,572 - -0.6 944,573 + +0.1 944,573 + +0.0 944,573 + +0.2 944,574 - -0.7 944,575 + -0.1 944,577 - +0.3 944,578 + +1.0 944,585 - -1.8 944,624 + +0.8 944,658 + -0.4 944,708 - +0.5 944,709 + -0.4 944,715 + +2.4 944,730 + +0.6 944,792 + MMJJ_RS05090 0.26 +0.6 944,843 + MMJJ_RS05090 0.43 -0.1 944,907 - MMJJ_RS05090 0.65 +1.3 944,907 - +2.1 944,916 - MMJJ_RS05090 0.68 -0.4 944,917 + MMJJ_RS05090 0.68 +1.7 944,920 + MMJJ_RS05090 0.69 +1.1 944,923 - MMJJ_RS05090 0.70 +0.7 944,938 - MMJJ_RS05090 0.75 +0.4 944,939 + MMJJ_RS05090 0.75 +0.6 944,945 - MMJJ_RS05090 0.77 -1.0 944,945 - MMJJ_RS05090 0.77 -0.2 944,945 - MMJJ_RS05090 0.77 +0.1 944,978 + MMJJ_RS05090 0.88 -0.1 944,979 - MMJJ_RS05090 0.89 +0.2 945,035 - +0.1 945,035 - +0.6 945,036 + +0.3 945,037 - +0.3 945,037 - +1.0 945,037 - +0.6 945,037 - -0.8 945,038 + +1.2 945,059 - +0.2 945,060 + -1.1 945,060 + -0.6 945,066 + +0.6 945,066 + +0.9 945,070 + +0.0 945,209 + MMJJ_RS05095 0.11 +0.7 945,235 + MMJJ_RS05095 0.13 -1.8 945,288 - MMJJ_RS05095 0.17 +0.5 945,330 - MMJJ_RS05095 0.21 +0.5 945,331 + MMJJ_RS05095 0.21 -0.2 945,340 + MMJJ_RS05095 0.22 -2.3 945,348 - MMJJ_RS05095 0.22 +0.3 945,348 - MMJJ_RS05095 0.22 -0.2 945,348 - MMJJ_RS05095 0.22 +0.5 945,348 - MMJJ_RS05095 0.22 -0.4 945,348 - MMJJ_RS05095 0.22 -0.2 945,348 - MMJJ_RS05095 0.22 +0.0 945,348 - MMJJ_RS05095 0.22 -0.0 945,348 - MMJJ_RS05095 0.22 -0.4 945,348 - MMJJ_RS05095 0.22 +1.2 945,349 + MMJJ_RS05095 0.22 +0.4 945,349 + MMJJ_RS05095 0.22 -0.0 945,349 + MMJJ_RS05095 0.22 -0.2 945,349 + MMJJ_RS05095 0.22 +0.2 945,349 + MMJJ_RS05095 0.22 +0.7 945,349 + MMJJ_RS05095 0.22 +1.0 945,349 + MMJJ_RS05095 0.22 +0.5 945,349 + MMJJ_RS05095 0.22 +0.5 945,349 + MMJJ_RS05095 0.22 +1.0 945,349 + MMJJ_RS05095 0.22 +0.3 945,349 + MMJJ_RS05095 0.22 -0.6 945,349 + MMJJ_RS05095 0.22 +0.8 945,349 + MMJJ_RS05095 0.22 +0.6 945,387 - MMJJ_RS05095 0.25 -0.6 945,388 + MMJJ_RS05095 0.26 -0.9 945,394 + MMJJ_RS05095 0.26 -0.1 945,420 - MMJJ_RS05095 0.28 +0.8 945,441 - MMJJ_RS05095 0.30 +1.5 945,441 - MMJJ_RS05095 0.30 +0.9
Or see this region's nucleotide sequence