Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03920 and MMJJ_RS03925 are separated by 256 nucleotides MMJJ_RS03925 and MMJJ_RS03930 are separated by 13 nucleotides MMJJ_RS03930 and MMJJ_RS03935 are separated by 19 nucleotides
MMJJ_RS03920: MMJJ_RS03920 - nickel pincer cofactor biosynthesis protein LarC, at 731,783 to 732,997
_RS03920
MMJJ_RS03925: MMJJ_RS03925 - tRNA pseudouridine(38-40) synthase TruA, at 733,254 to 734,042
_RS03925
MMJJ_RS03930: MMJJ_RS03930 - hypothetical protein, at 734,056 to 734,772
_RS03930
MMJJ_RS03935: MMJJ_RS03935 - NAAT family transporter, at 734,792 to 735,433
_RS03935
Position (kb) 733
734
735 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 732.276 kb on + strand, within MMJJ_RS03920 at 732.338 kb on - strand, within MMJJ_RS03920 at 732.383 kb on - strand, within MMJJ_RS03920 at 732.384 kb on + strand, within MMJJ_RS03920 at 732.384 kb on + strand, within MMJJ_RS03920 at 732.425 kb on - strand, within MMJJ_RS03920 at 732.425 kb on - strand, within MMJJ_RS03920 at 732.426 kb on + strand, within MMJJ_RS03920 at 732.426 kb on + strand, within MMJJ_RS03920 at 732.451 kb on - strand, within MMJJ_RS03920 at 732.506 kb on + strand, within MMJJ_RS03920 at 732.596 kb on - strand, within MMJJ_RS03920 at 732.596 kb on - strand, within MMJJ_RS03920 at 732.596 kb on - strand, within MMJJ_RS03920 at 732.597 kb on + strand, within MMJJ_RS03920 at 732.597 kb on + strand, within MMJJ_RS03920 at 732.614 kb on - strand, within MMJJ_RS03920 at 732.614 kb on - strand, within MMJJ_RS03920 at 732.614 kb on - strand, within MMJJ_RS03920 at 732.614 kb on - strand, within MMJJ_RS03920 at 732.683 kb on - strand, within MMJJ_RS03920 at 732.723 kb on + strand, within MMJJ_RS03920 at 732.736 kb on - strand, within MMJJ_RS03920 at 732.736 kb on - strand, within MMJJ_RS03920 at 732.736 kb on - strand, within MMJJ_RS03920 at 732.736 kb on - strand, within MMJJ_RS03920 at 732.737 kb on + strand, within MMJJ_RS03920 at 732.737 kb on + strand, within MMJJ_RS03920 at 732.737 kb on + strand, within MMJJ_RS03920 at 732.737 kb on + strand, within MMJJ_RS03920 at 732.741 kb on + strand, within MMJJ_RS03920 at 732.746 kb on + strand, within MMJJ_RS03920 at 732.756 kb on + strand, within MMJJ_RS03920 at 732.850 kb on - strand, within MMJJ_RS03920 at 732.850 kb on - strand, within MMJJ_RS03920 at 732.946 kb on - strand at 732.947 kb on + strand at 733.001 kb on + strand at 733.020 kb on + strand at 733.029 kb on - strand at 733.029 kb on - strand at 733.029 kb on - strand at 733.030 kb on + strand at 733.035 kb on - strand at 733.035 kb on - strand at 733.035 kb on - strand at 733.036 kb on + strand at 733.052 kb on + strand at 733.096 kb on + strand at 733.104 kb on - strand at 733.104 kb on - strand at 733.105 kb on + strand at 733.105 kb on + strand at 733.130 kb on - strand at 733.143 kb on + strand at 733.149 kb on + strand at 733.176 kb on + strand at 733.183 kb on - strand at 733.184 kb on + strand at 733.184 kb on + strand at 733.188 kb on - strand at 733.188 kb on - strand at 733.188 kb on - strand at 733.189 kb on + strand at 733.189 kb on + strand at 733.190 kb on - strand at 733.190 kb on - strand at 733.191 kb on + strand at 733.191 kb on + strand at 733.192 kb on - strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.193 kb on + strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.194 kb on - strand at 733.195 kb on + strand at 733.195 kb on + strand at 733.195 kb on + strand at 733.195 kb on + strand at 733.195 kb on + strand at 733.195 kb on + strand at 733.200 kb on - strand at 733.206 kb on + strand at 733.239 kb on - strand at 733.242 kb on + strand at 733.251 kb on + strand at 733.251 kb on + strand at 733.253 kb on - strand at 733.253 kb on - strand at 733.253 kb on - strand at 733.253 kb on - strand at 733.253 kb on - strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.254 kb on + strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.257 kb on - strand at 733.258 kb on + strand at 733.261 kb on - strand at 733.261 kb on - strand at 733.261 kb on - strand at 733.262 kb on + strand at 733.262 kb on + strand at 733.262 kb on + strand at 733.276 kb on + strand at 733.276 kb on + strand at 733.364 kb on + strand, within MMJJ_RS03925 at 733.364 kb on + strand, within MMJJ_RS03925 at 733.364 kb on + strand, within MMJJ_RS03925 at 733.377 kb on + strand, within MMJJ_RS03925 at 733.389 kb on - strand, within MMJJ_RS03925 at 733.389 kb on - strand, within MMJJ_RS03925 at 733.390 kb on + strand, within MMJJ_RS03925 at 733.390 kb on + strand, within MMJJ_RS03925 at 733.418 kb on + strand, within MMJJ_RS03925 at 733.438 kb on - strand, within MMJJ_RS03925 at 733.438 kb on - strand, within MMJJ_RS03925 at 733.438 kb on - strand, within MMJJ_RS03925 at 733.439 kb on + strand, within MMJJ_RS03925 at 733.439 kb on + strand, within MMJJ_RS03925 at 733.439 kb on + strand, within MMJJ_RS03925 at 733.443 kb on - strand, within MMJJ_RS03925 at 733.444 kb on + strand, within MMJJ_RS03925 at 733.444 kb on + strand, within MMJJ_RS03925 at 733.490 kb on - strand, within MMJJ_RS03925 at 733.490 kb on - strand, within MMJJ_RS03925 at 733.551 kb on - strand, within MMJJ_RS03925 at 733.566 kb on - strand, within MMJJ_RS03925 at 733.568 kb on - strand, within MMJJ_RS03925 at 733.568 kb on - strand, within MMJJ_RS03925 at 733.569 kb on + strand, within MMJJ_RS03925 at 733.601 kb on + strand, within MMJJ_RS03925 at 733.601 kb on + strand, within MMJJ_RS03925 at 733.638 kb on - strand, within MMJJ_RS03925 at 733.639 kb on + strand, within MMJJ_RS03925 at 733.675 kb on - strand at 733.744 kb on - strand, within MMJJ_RS03925 at 733.744 kb on - strand, within MMJJ_RS03925 at 733.745 kb on + strand, within MMJJ_RS03925 at 733.745 kb on + strand, within MMJJ_RS03925 at 733.775 kb on - strand, within MMJJ_RS03925 at 733.776 kb on + strand, within MMJJ_RS03925 at 733.784 kb on - strand, within MMJJ_RS03925 at 733.786 kb on - strand, within MMJJ_RS03925 at 733.786 kb on - strand, within MMJJ_RS03925 at 733.847 kb on + strand, within MMJJ_RS03925 at 733.847 kb on + strand, within MMJJ_RS03925 at 733.847 kb on + strand, within MMJJ_RS03925 at 733.903 kb on - strand, within MMJJ_RS03925 at 733.903 kb on - strand, within MMJJ_RS03925 at 733.903 kb on - strand, within MMJJ_RS03925 at 733.944 kb on + strand, within MMJJ_RS03925 at 733.947 kb on - strand, within MMJJ_RS03925 at 733.947 kb on - strand, within MMJJ_RS03925 at 733.948 kb on + strand, within MMJJ_RS03925 at 733.948 kb on + strand, within MMJJ_RS03925 at 733.948 kb on + strand, within MMJJ_RS03925 at 734.052 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.062 kb on - strand at 734.063 kb on + strand at 734.063 kb on + strand at 734.063 kb on + strand at 734.063 kb on + strand at 734.063 kb on + strand at 734.076 kb on + strand at 734.076 kb on + strand at 734.087 kb on - strand at 734.088 kb on + strand at 734.088 kb on + strand at 734.109 kb on - strand at 734.150 kb on - strand, within MMJJ_RS03930 at 734.171 kb on - strand, within MMJJ_RS03930 at 734.185 kb on + strand, within MMJJ_RS03930 at 734.185 kb on + strand, within MMJJ_RS03930 at 734.185 kb on + strand, within MMJJ_RS03930 at 734.211 kb on + strand, within MMJJ_RS03930 at 734.270 kb on - strand, within MMJJ_RS03930 at 734.275 kb on - strand, within MMJJ_RS03930 at 734.316 kb on - strand, within MMJJ_RS03930 at 734.317 kb on + strand, within MMJJ_RS03930 at 734.350 kb on - strand, within MMJJ_RS03930 at 734.351 kb on + strand, within MMJJ_RS03930 at 734.406 kb on - strand, within MMJJ_RS03930 at 734.410 kb on - strand, within MMJJ_RS03930 at 734.410 kb on - strand, within MMJJ_RS03930 at 734.411 kb on + strand, within MMJJ_RS03930 at 734.411 kb on + strand, within MMJJ_RS03930 at 734.411 kb on + strand, within MMJJ_RS03930 at 734.444 kb on - strand, within MMJJ_RS03930 at 734.444 kb on - strand, within MMJJ_RS03930 at 734.444 kb on - strand, within MMJJ_RS03930 at 734.444 kb on - strand, within MMJJ_RS03930 at 734.476 kb on - strand, within MMJJ_RS03930 at 734.544 kb on - strand, within MMJJ_RS03930 at 734.545 kb on + strand, within MMJJ_RS03930 at 734.644 kb on - strand, within MMJJ_RS03930 at 734.645 kb on + strand, within MMJJ_RS03930 at 734.673 kb on - strand, within MMJJ_RS03930 at 734.673 kb on - strand, within MMJJ_RS03930 at 734.673 kb on - strand, within MMJJ_RS03930 at 734.674 kb on + strand, within MMJJ_RS03930 at 734.675 kb on - strand, within MMJJ_RS03930 at 734.675 kb on - strand, within MMJJ_RS03930 at 734.676 kb on + strand, within MMJJ_RS03930 at 734.696 kb on + strand, within MMJJ_RS03930 at 734.697 kb on - strand, within MMJJ_RS03930 at 734.770 kb on - strand at 734.770 kb on - strand at 734.771 kb on + strand at 734.771 kb on + strand at 734.791 kb on - strand at 734.800 kb on + strand at 734.807 kb on + strand at 734.868 kb on + strand, within MMJJ_RS03935 at 734.871 kb on - strand, within MMJJ_RS03935 at 734.871 kb on - strand, within MMJJ_RS03935 at 734.872 kb on + strand, within MMJJ_RS03935 at 734.881 kb on - strand, within MMJJ_RS03935 at 734.888 kb on - strand, within MMJJ_RS03935 at 734.920 kb on - strand, within MMJJ_RS03935 at 734.920 kb on - strand, within MMJJ_RS03935 at 734.921 kb on + strand, within MMJJ_RS03935 at 734.926 kb on - strand, within MMJJ_RS03935 at 734.927 kb on + strand, within MMJJ_RS03935 at 734.949 kb on - strand, within MMJJ_RS03935 at 734.949 kb on - strand, within MMJJ_RS03935 at 734.950 kb on + strand, within MMJJ_RS03935 at 734.950 kb on + strand, within MMJJ_RS03935 at 734.950 kb on + strand, within MMJJ_RS03935 at 735.037 kb on + strand, within MMJJ_RS03935 at 735.037 kb on + strand, within MMJJ_RS03935
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 732,276 + MMJJ_RS03920 0.41 -1.8 732,338 - MMJJ_RS03920 0.46 -1.2 732,383 - MMJJ_RS03920 0.49 +2.3 732,384 + MMJJ_RS03920 0.49 -2.1 732,384 + MMJJ_RS03920 0.49 -1.3 732,425 - MMJJ_RS03920 0.53 -1.9 732,425 - MMJJ_RS03920 0.53 -0.2 732,426 + MMJJ_RS03920 0.53 -0.2 732,426 + MMJJ_RS03920 0.53 -1.2 732,451 - MMJJ_RS03920 0.55 -0.0 732,506 + MMJJ_RS03920 0.60 -1.7 732,596 - MMJJ_RS03920 0.67 -1.2 732,596 - MMJJ_RS03920 0.67 -0.7 732,596 - MMJJ_RS03920 0.67 -2.5 732,597 + MMJJ_RS03920 0.67 -0.1 732,597 + MMJJ_RS03920 0.67 -1.8 732,614 - MMJJ_RS03920 0.68 -1.7 732,614 - MMJJ_RS03920 0.68 -1.3 732,614 - MMJJ_RS03920 0.68 +1.0 732,614 - MMJJ_RS03920 0.68 -1.8 732,683 - MMJJ_RS03920 0.74 -0.6 732,723 + MMJJ_RS03920 0.77 -1.9 732,736 - MMJJ_RS03920 0.78 -2.8 732,736 - MMJJ_RS03920 0.78 +0.3 732,736 - MMJJ_RS03920 0.78 -2.7 732,736 - MMJJ_RS03920 0.78 -0.4 732,737 + MMJJ_RS03920 0.79 -0.3 732,737 + MMJJ_RS03920 0.79 +0.3 732,737 + MMJJ_RS03920 0.79 +0.4 732,737 + MMJJ_RS03920 0.79 +1.6 732,741 + MMJJ_RS03920 0.79 -1.0 732,746 + MMJJ_RS03920 0.79 -1.3 732,756 + MMJJ_RS03920 0.80 -0.9 732,850 - MMJJ_RS03920 0.88 -1.6 732,850 - MMJJ_RS03920 0.88 -1.0 732,946 - -0.4 732,947 + +0.3 733,001 + -0.9 733,020 + -0.1 733,029 - -0.6 733,029 - +0.6 733,029 - -1.6 733,030 + +0.6 733,035 - +0.8 733,035 - -0.1 733,035 - +0.0 733,036 + -0.2 733,052 + -1.0 733,096 + +0.5 733,104 - +0.0 733,104 - -1.3 733,105 + +0.6 733,105 + -0.2 733,130 - -1.0 733,143 + +0.4 733,149 + -0.0 733,176 + -0.5 733,183 - +0.7 733,184 + +0.5 733,184 + -0.9 733,188 - -0.1 733,188 - +0.8 733,188 - +0.4 733,189 + +0.3 733,189 + +0.7 733,190 - +0.3 733,190 - +0.7 733,191 + +0.6 733,191 + +1.4 733,192 - -0.8 733,193 + -1.3 733,193 + +0.1 733,193 + -0.9 733,193 + -0.1 733,193 + -0.9 733,193 + +0.4 733,193 + -1.3 733,193 + -1.1 733,193 + +0.6 733,193 + +0.4 733,194 - +0.0 733,194 - -0.8 733,194 - +0.6 733,194 - +0.1 733,194 - -0.0 733,194 - -0.6 733,194 - +0.1 733,194 - +0.4 733,195 + -0.4 733,195 + +1.1 733,195 + +0.7 733,195 + -1.3 733,195 + +0.2 733,195 + -0.2 733,200 - +0.4 733,206 + +0.1 733,239 - -0.2 733,242 + -0.6 733,251 + +0.2 733,251 + +0.4 733,253 - -0.3 733,253 - -0.1 733,253 - -1.3 733,253 - +0.3 733,253 - +0.2 733,254 + -0.8 733,254 + -0.6 733,254 + +0.6 733,254 + +0.0 733,254 + -0.5 733,254 + -0.8 733,254 + -1.2 733,254 + +0.2 733,254 + -0.6 733,257 - +0.3 733,257 - -0.4 733,257 - +1.1 733,257 - -0.1 733,257 - +0.1 733,257 - -2.9 733,257 - -2.2 733,258 + +1.0 733,261 - +0.1 733,261 - -0.8 733,261 - +0.0 733,262 + -1.0 733,262 + +0.3 733,262 + +0.4 733,276 + -0.4 733,276 + -1.4 733,364 + MMJJ_RS03925 0.14 -1.6 733,364 + MMJJ_RS03925 0.14 -1.6 733,364 + MMJJ_RS03925 0.14 +0.9 733,377 + MMJJ_RS03925 0.16 +0.2 733,389 - MMJJ_RS03925 0.17 -0.4 733,389 - MMJJ_RS03925 0.17 +0.0 733,390 + MMJJ_RS03925 0.17 -0.0 733,390 + MMJJ_RS03925 0.17 -1.4 733,418 + MMJJ_RS03925 0.21 -1.3 733,438 - MMJJ_RS03925 0.23 -0.3 733,438 - MMJJ_RS03925 0.23 -0.2 733,438 - MMJJ_RS03925 0.23 -0.3 733,439 + MMJJ_RS03925 0.23 -0.1 733,439 + MMJJ_RS03925 0.23 +0.0 733,439 + MMJJ_RS03925 0.23 -0.1 733,443 - MMJJ_RS03925 0.24 -0.9 733,444 + MMJJ_RS03925 0.24 +0.2 733,444 + MMJJ_RS03925 0.24 -1.0 733,490 - MMJJ_RS03925 0.30 +0.9 733,490 - MMJJ_RS03925 0.30 +0.8 733,551 - MMJJ_RS03925 0.38 -1.3 733,566 - MMJJ_RS03925 0.40 -0.6 733,568 - MMJJ_RS03925 0.40 +0.3 733,568 - MMJJ_RS03925 0.40 +0.1 733,569 + MMJJ_RS03925 0.40 -0.1 733,601 + MMJJ_RS03925 0.44 -0.9 733,601 + MMJJ_RS03925 0.44 -0.9 733,638 - MMJJ_RS03925 0.49 +1.0 733,639 + MMJJ_RS03925 0.49 -0.0 733,675 - -0.8 733,744 - MMJJ_RS03925 0.62 -1.7 733,744 - MMJJ_RS03925 0.62 +0.9 733,745 + MMJJ_RS03925 0.62 -1.2 733,745 + MMJJ_RS03925 0.62 +0.1 733,775 - MMJJ_RS03925 0.66 -3.2 733,776 + MMJJ_RS03925 0.66 -1.4 733,784 - MMJJ_RS03925 0.67 +0.3 733,786 - MMJJ_RS03925 0.67 +1.1 733,786 - MMJJ_RS03925 0.67 -1.6 733,847 + MMJJ_RS03925 0.75 -0.0 733,847 + MMJJ_RS03925 0.75 -0.7 733,847 + MMJJ_RS03925 0.75 +0.1 733,903 - MMJJ_RS03925 0.82 -1.5 733,903 - MMJJ_RS03925 0.82 -1.1 733,903 - MMJJ_RS03925 0.82 +1.2 733,944 + MMJJ_RS03925 0.87 -1.3 733,947 - MMJJ_RS03925 0.88 -0.0 733,947 - MMJJ_RS03925 0.88 +0.1 733,948 + MMJJ_RS03925 0.88 -0.0 733,948 + MMJJ_RS03925 0.88 -0.6 733,948 + MMJJ_RS03925 0.88 -2.4 734,052 - -1.2 734,062 - -0.4 734,062 - +1.4 734,062 - -1.2 734,062 - +1.0 734,062 - +0.3 734,062 - -0.6 734,062 - -0.2 734,063 + -0.2 734,063 + +0.2 734,063 + +0.2 734,063 + +0.5 734,063 + +0.3 734,076 + -1.8 734,076 + +0.5 734,087 - +0.0 734,088 + -1.0 734,088 + -0.7 734,109 - -0.2 734,150 - MMJJ_RS03930 0.13 -1.1 734,171 - MMJJ_RS03930 0.16 -0.1 734,185 + MMJJ_RS03930 0.18 -1.0 734,185 + MMJJ_RS03930 0.18 +0.3 734,185 + MMJJ_RS03930 0.18 -0.6 734,211 + MMJJ_RS03930 0.22 -0.6 734,270 - MMJJ_RS03930 0.30 -1.1 734,275 - MMJJ_RS03930 0.31 -1.6 734,316 - MMJJ_RS03930 0.36 -0.4 734,317 + MMJJ_RS03930 0.36 +0.0 734,350 - MMJJ_RS03930 0.41 -1.5 734,351 + MMJJ_RS03930 0.41 -0.7 734,406 - MMJJ_RS03930 0.49 -1.5 734,410 - MMJJ_RS03930 0.49 -1.2 734,410 - MMJJ_RS03930 0.49 +3.0 734,411 + MMJJ_RS03930 0.50 +0.5 734,411 + MMJJ_RS03930 0.50 -1.1 734,411 + MMJJ_RS03930 0.50 -1.2 734,444 - MMJJ_RS03930 0.54 -0.1 734,444 - MMJJ_RS03930 0.54 -2.3 734,444 - MMJJ_RS03930 0.54 -1.5 734,444 - MMJJ_RS03930 0.54 -0.4 734,476 - MMJJ_RS03930 0.59 +0.8 734,544 - MMJJ_RS03930 0.68 -1.7 734,545 + MMJJ_RS03930 0.68 -1.1 734,644 - MMJJ_RS03930 0.82 -1.3 734,645 + MMJJ_RS03930 0.82 -1.5 734,673 - MMJJ_RS03930 0.86 -1.1 734,673 - MMJJ_RS03930 0.86 +0.2 734,673 - MMJJ_RS03930 0.86 +0.2 734,674 + MMJJ_RS03930 0.86 -1.6 734,675 - MMJJ_RS03930 0.86 -1.2 734,675 - MMJJ_RS03930 0.86 -3.3 734,676 + MMJJ_RS03930 0.86 -0.2 734,696 + MMJJ_RS03930 0.89 -1.3 734,697 - MMJJ_RS03930 0.89 -0.2 734,770 - +0.7 734,770 - -2.3 734,771 + -0.3 734,771 + +0.1 734,791 - -0.3 734,800 + -0.4 734,807 + -0.5 734,868 + MMJJ_RS03935 0.12 +0.0 734,871 - MMJJ_RS03935 0.12 -1.2 734,871 - MMJJ_RS03935 0.12 -0.4 734,872 + MMJJ_RS03935 0.12 -2.0 734,881 - MMJJ_RS03935 0.14 -0.6 734,888 - MMJJ_RS03935 0.15 -1.7 734,920 - MMJJ_RS03935 0.20 -0.4 734,920 - MMJJ_RS03935 0.20 -0.1 734,921 + MMJJ_RS03935 0.20 -1.5 734,926 - MMJJ_RS03935 0.21 -1.2 734,927 + MMJJ_RS03935 0.21 +0.4 734,949 - MMJJ_RS03935 0.24 +1.8 734,949 - MMJJ_RS03935 0.24 -1.4 734,950 + MMJJ_RS03935 0.25 +0.3 734,950 + MMJJ_RS03935 0.25 -1.2 734,950 + MMJJ_RS03935 0.25 -0.7 735,037 + MMJJ_RS03935 0.38 -1.8 735,037 + MMJJ_RS03935 0.38 -1.1
Or see this region's nucleotide sequence