Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03580 and MMJJ_RS03585 are separated by 29 nucleotides MMJJ_RS03585 and MMJJ_RS03590 are separated by 16 nucleotides MMJJ_RS03590 and MMJJ_RS03595 are separated by 47 nucleotides MMJJ_RS03595 and MMJJ_RS03600 are separated by 70 nucleotides
MMJJ_RS03580: MMJJ_RS03580 - TIGR00303 family protein, at 668,823 to 669,875
_RS03580
MMJJ_RS03585: MMJJ_RS03585 - methanogenesis marker 6 protein, at 669,905 to 670,351
_RS03585
MMJJ_RS03590: MMJJ_RS03590 - restriction endonuclease, at 670,368 to 670,775
_RS03590
MMJJ_RS03595: MMJJ_RS03595 - hypothetical protein, at 670,823 to 671,236
_RS03595
MMJJ_RS03600: MMJJ_RS03600 - TrmJ/YjtD family RNA methyltransferase, at 671,307 to 672,011
_RS03600
Position (kb) 669
670
671 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 668.922 kb on - strand at 668.922 kb on - strand at 668.922 kb on - strand at 668.923 kb on + strand at 668.995 kb on - strand, within MMJJ_RS03580 at 669.239 kb on - strand, within MMJJ_RS03580 at 669.245 kb on - strand, within MMJJ_RS03580 at 669.255 kb on + strand, within MMJJ_RS03580 at 669.260 kb on + strand, within MMJJ_RS03580 at 669.263 kb on + strand, within MMJJ_RS03580 at 669.339 kb on + strand, within MMJJ_RS03580 at 669.736 kb on + strand, within MMJJ_RS03580 at 669.829 kb on + strand at 669.850 kb on - strand at 669.851 kb on + strand at 669.968 kb on - strand, within MMJJ_RS03585 at 670.291 kb on - strand, within MMJJ_RS03585 at 670.336 kb on + strand at 670.336 kb on + strand at 670.336 kb on + strand at 670.349 kb on - strand at 670.350 kb on + strand at 670.376 kb on - strand at 670.377 kb on + strand at 670.381 kb on + strand at 670.394 kb on - strand at 670.394 kb on - strand at 670.477 kb on - strand, within MMJJ_RS03590 at 670.508 kb on - strand, within MMJJ_RS03590 at 670.545 kb on - strand, within MMJJ_RS03590 at 670.546 kb on + strand, within MMJJ_RS03590 at 670.626 kb on + strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.644 kb on + strand, within MMJJ_RS03590 at 670.645 kb on - strand, within MMJJ_RS03590 at 670.646 kb on + strand, within MMJJ_RS03590 at 670.745 kb on - strand at 670.773 kb on - strand at 670.773 kb on - strand at 670.774 kb on + strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.777 kb on + strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.779 kb on + strand at 670.779 kb on + strand at 670.792 kb on + strand at 670.839 kb on + strand at 670.866 kb on + strand, within MMJJ_RS03595 at 670.866 kb on + strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595 at 670.889 kb on - strand, within MMJJ_RS03595 at 670.890 kb on + strand, within MMJJ_RS03595 at 670.968 kb on + strand, within MMJJ_RS03595 at 670.984 kb on - strand, within MMJJ_RS03595 at 671.019 kb on + strand, within MMJJ_RS03595 at 671.166 kb on + strand, within MMJJ_RS03595 at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.257 kb on - strand at 671.257 kb on - strand at 671.258 kb on + strand at 671.261 kb on + strand at 671.266 kb on - strand at 671.270 kb on + strand at 671.276 kb on + strand at 671.276 kb on + strand at 671.309 kb on + strand at 671.321 kb on - strand at 671.322 kb on + strand at 671.328 kb on + strand at 671.337 kb on - strand at 671.338 kb on + strand at 671.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 668,922 - -2.8 668,922 - -2.3 668,922 - -2.2 668,923 + -2.8 668,995 - MMJJ_RS03580 0.16 -3.8 669,239 - MMJJ_RS03580 0.40 -2.6 669,245 - MMJJ_RS03580 0.40 -2.2 669,255 + MMJJ_RS03580 0.41 -1.8 669,260 + MMJJ_RS03580 0.42 -3.5 669,263 + MMJJ_RS03580 0.42 -2.3 669,339 + MMJJ_RS03580 0.49 -1.6 669,736 + MMJJ_RS03580 0.87 -2.3 669,829 + +1.0 669,850 - -0.9 669,851 + -3.3 669,968 - MMJJ_RS03585 0.14 -1.3 670,291 - MMJJ_RS03585 0.86 -2.5 670,336 + -2.8 670,336 + +0.6 670,336 + +0.5 670,349 - -1.0 670,350 + +0.5 670,376 - -1.7 670,377 + +1.2 670,381 + +1.0 670,394 - -0.5 670,394 - +0.8 670,477 - MMJJ_RS03590 0.27 -0.1 670,508 - MMJJ_RS03590 0.34 +0.4 670,545 - MMJJ_RS03590 0.43 -0.6 670,546 + MMJJ_RS03590 0.44 +0.7 670,626 + MMJJ_RS03590 0.63 -1.0 670,639 - MMJJ_RS03590 0.66 +1.0 670,639 - MMJJ_RS03590 0.66 -0.8 670,644 + MMJJ_RS03590 0.68 -1.0 670,645 - MMJJ_RS03590 0.68 +0.7 670,646 + MMJJ_RS03590 0.68 -1.8 670,745 - -3.2 670,773 - -0.6 670,773 - +0.3 670,774 + +0.6 670,776 - +1.1 670,776 - +0.6 670,776 - -0.3 670,776 - +0.1 670,777 + +0.5 670,778 - +1.7 670,778 - -0.0 670,778 - +0.2 670,779 + -0.3 670,779 + +0.4 670,792 + -0.8 670,839 + +1.9 670,866 + MMJJ_RS03595 0.10 +1.8 670,866 + MMJJ_RS03595 0.10 -0.6 670,869 - MMJJ_RS03595 0.11 -1.4 670,869 - MMJJ_RS03595 0.11 -0.7 670,869 - MMJJ_RS03595 0.11 +0.4 670,870 + MMJJ_RS03595 0.11 -0.4 670,870 + MMJJ_RS03595 0.11 +0.3 670,889 - MMJJ_RS03595 0.16 +0.1 670,890 + MMJJ_RS03595 0.16 -2.3 670,968 + MMJJ_RS03595 0.35 +0.2 670,984 - MMJJ_RS03595 0.39 +0.1 671,019 + MMJJ_RS03595 0.47 -0.9 671,166 + MMJJ_RS03595 0.83 -1.5 671,229 + -0.1 671,229 + -1.5 671,229 + -0.6 671,229 + -1.1 671,229 + -1.5 671,230 - -1.1 671,230 - -0.0 671,230 - -0.2 671,257 - -0.2 671,257 - -0.4 671,258 + +0.5 671,261 + -0.9 671,266 - +1.2 671,270 + -0.0 671,276 + +0.2 671,276 + -0.3 671,309 + -0.4 671,321 - +1.1 671,322 + -0.9 671,328 + -0.3 671,337 - +0.4 671,338 + +0.6 671,341 + -2.2
Or see this region's nucleotide sequence