Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02265 and MMJJ_RS02270 are separated by 157 nucleotides MMJJ_RS02270 and MMJJ_RS02275 overlap by 14 nucleotides MMJJ_RS02275 and MMJJ_RS02280 are separated by 231 nucleotides
MMJJ_RS02265: MMJJ_RS02265 - hypothetical protein, at 427,859 to 429,328
_RS02265
MMJJ_RS02270: MMJJ_RS02270 - HEPN domain-containing protein, at 429,486 to 429,995
_RS02270
MMJJ_RS02275: MMJJ_RS02275 - nucleotidyltransferase domain-containing protein, at 429,982 to 430,599
_RS02275
MMJJ_RS02280: MMJJ_RS02280 - sulfate permease, at 430,831 to 432,489
_RS02280
Position (kb) 429
430
431 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 428.988 kb on - strand, within MMJJ_RS02265 at 429.030 kb on + strand, within MMJJ_RS02265 at 429.125 kb on - strand, within MMJJ_RS02265 at 429.181 kb on - strand, within MMJJ_RS02265 at 429.235 kb on - strand at 429.289 kb on - strand at 429.302 kb on + strand at 429.309 kb on + strand at 429.331 kb on + strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.358 kb on + strand at 429.358 kb on + strand at 429.359 kb on - strand at 429.359 kb on - strand at 429.360 kb on + strand at 429.365 kb on - strand at 429.365 kb on - strand at 429.365 kb on - strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.382 kb on - strand at 429.383 kb on + strand at 429.383 kb on + strand at 429.394 kb on + strand at 429.406 kb on - strand at 429.410 kb on - strand at 429.423 kb on + strand at 429.433 kb on + strand at 429.435 kb on + strand at 429.458 kb on + strand at 429.516 kb on - strand at 429.546 kb on - strand, within MMJJ_RS02270 at 429.547 kb on + strand, within MMJJ_RS02270 at 429.610 kb on + strand, within MMJJ_RS02270 at 429.638 kb on - strand, within MMJJ_RS02270 at 429.703 kb on + strand, within MMJJ_RS02270 at 429.803 kb on - strand, within MMJJ_RS02270 at 429.843 kb on - strand, within MMJJ_RS02270 at 429.858 kb on + strand, within MMJJ_RS02270 at 429.943 kb on - strand, within MMJJ_RS02270 at 429.976 kb on - strand at 430.109 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.114 kb on - strand, within MMJJ_RS02275 at 430.350 kb on - strand, within MMJJ_RS02275 at 430.350 kb on - strand, within MMJJ_RS02275 at 430.424 kb on - strand, within MMJJ_RS02275 at 430.447 kb on - strand, within MMJJ_RS02275 at 430.536 kb on - strand, within MMJJ_RS02275 at 430.566 kb on + strand at 430.568 kb on - strand at 430.569 kb on + strand at 430.579 kb on - strand at 430.613 kb on + strand at 430.619 kb on + strand at 430.622 kb on - strand at 430.622 kb on - strand at 430.622 kb on - strand at 430.623 kb on + strand at 430.623 kb on + strand at 430.624 kb on - strand at 430.680 kb on - strand at 430.680 kb on - strand at 430.693 kb on - strand at 430.693 kb on - strand at 430.693 kb on - strand at 430.694 kb on + strand at 430.698 kb on + strand at 430.699 kb on - strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.705 kb on + strand at 430.710 kb on + strand at 430.722 kb on + strand at 430.736 kb on + strand at 430.739 kb on - strand at 430.740 kb on + strand at 430.740 kb on + strand at 430.740 kb on + strand at 430.741 kb on - strand at 430.741 kb on - strand at 430.742 kb on + strand at 430.743 kb on - strand at 430.744 kb on + strand at 430.762 kb on - strand at 430.764 kb on - strand at 430.765 kb on + strand at 430.765 kb on + strand at 430.787 kb on + strand at 430.790 kb on - strand at 430.796 kb on + strand at 430.796 kb on + strand at 430.808 kb on + strand at 430.808 kb on + strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.829 kb on + strand at 430.829 kb on + strand at 430.846 kb on + strand at 430.878 kb on + strand at 430.901 kb on - strand at 430.902 kb on + strand at 430.964 kb on - strand at 430.992 kb on + strand at 430.992 kb on + strand at 431.073 kb on - strand, within MMJJ_RS02280 at 431.074 kb on + strand, within MMJJ_RS02280 at 431.126 kb on - strand, within MMJJ_RS02280 at 431.160 kb on - strand, within MMJJ_RS02280 at 431.196 kb on - strand, within MMJJ_RS02280 at 431.197 kb on + strand, within MMJJ_RS02280 at 431.217 kb on + strand, within MMJJ_RS02280 at 431.295 kb on - strand, within MMJJ_RS02280 at 431.295 kb on - strand, within MMJJ_RS02280 at 431.299 kb on - strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.310 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.322 kb on + strand, within MMJJ_RS02280 at 431.358 kb on + strand, within MMJJ_RS02280 at 431.362 kb on + strand, within MMJJ_RS02280 at 431.408 kb on - strand, within MMJJ_RS02280 at 431.417 kb on - strand, within MMJJ_RS02280 at 431.442 kb on - strand, within MMJJ_RS02280 at 431.443 kb on + strand, within MMJJ_RS02280 at 431.443 kb on + strand at 431.443 kb on + strand, within MMJJ_RS02280 at 431.454 kb on + strand, within MMJJ_RS02280 at 431.476 kb on + strand, within MMJJ_RS02280 at 431.568 kb on - strand, within MMJJ_RS02280 at 431.588 kb on - strand, within MMJJ_RS02280
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 428,988 - MMJJ_RS02265 0.77 +1.4 429,030 + MMJJ_RS02265 0.80 +0.4 429,125 - MMJJ_RS02265 0.86 -2.1 429,181 - MMJJ_RS02265 0.90 -0.3 429,235 - -0.2 429,289 - -0.6 429,302 + -1.2 429,309 + -0.2 429,331 + -0.6 429,357 - -0.1 429,357 - +0.1 429,357 - -0.1 429,357 - +0.6 429,358 + +1.1 429,358 + -1.0 429,359 - -0.6 429,359 - -0.1 429,360 + +0.8 429,365 - -1.7 429,365 - +0.4 429,365 - -1.6 429,366 + +0.4 429,366 + -0.7 429,366 + +0.7 429,366 + +0.7 429,366 + -0.7 429,382 - +0.7 429,383 + -1.1 429,383 + +0.1 429,394 + +0.1 429,406 - +1.0 429,410 - +0.3 429,423 + -0.9 429,433 + +0.8 429,435 + +0.4 429,458 + +0.1 429,516 - +0.7 429,546 - MMJJ_RS02270 0.12 -0.5 429,547 + MMJJ_RS02270 0.12 +0.3 429,610 + MMJJ_RS02270 0.24 +0.2 429,638 - MMJJ_RS02270 0.30 -0.8 429,703 + MMJJ_RS02270 0.43 +0.4 429,803 - MMJJ_RS02270 0.62 +1.4 429,843 - MMJJ_RS02270 0.70 +0.6 429,858 + MMJJ_RS02270 0.73 +1.4 429,943 - MMJJ_RS02270 0.90 -0.0 429,976 - +0.5 430,109 + MMJJ_RS02275 0.21 -4.2 430,111 + MMJJ_RS02275 0.21 -2.9 430,111 + MMJJ_RS02275 0.21 -1.7 430,111 + MMJJ_RS02275 0.21 -2.7 430,114 - MMJJ_RS02275 0.21 -2.4 430,350 - MMJJ_RS02275 0.60 -2.8 430,350 - MMJJ_RS02275 0.60 -3.4 430,424 - MMJJ_RS02275 0.72 -2.2 430,447 - MMJJ_RS02275 0.75 -2.2 430,536 - MMJJ_RS02275 0.90 -1.9 430,566 + -1.9 430,568 - -2.2 430,569 + -2.0 430,579 - -2.4 430,613 + -4.4 430,619 + -4.3 430,622 - -2.7 430,622 - -3.4 430,622 - -4.4 430,623 + -0.7 430,623 + -2.4 430,624 - -4.6 430,680 - -0.0 430,680 - +0.8 430,693 - -0.1 430,693 - +0.4 430,693 - +1.8 430,694 + +0.0 430,698 + +0.4 430,699 - +0.7 430,700 + -0.4 430,700 + -1.0 430,700 + +1.6 430,700 + +1.3 430,705 + +0.8 430,710 + +0.4 430,722 + +0.0 430,736 + +0.9 430,739 - -0.5 430,740 + +0.7 430,740 + +0.0 430,740 + +0.4 430,741 - -0.1 430,741 - +0.2 430,742 + +0.8 430,743 - -0.4 430,744 + +0.3 430,762 - +0.9 430,764 - -0.2 430,765 + +0.1 430,765 + -0.8 430,787 + +0.4 430,790 - +2.2 430,796 + +0.4 430,796 + -0.7 430,808 + -0.4 430,808 + -0.2 430,828 - -0.4 430,828 - +0.4 430,828 - -0.0 430,828 - -1.3 430,828 - +0.5 430,828 - +0.8 430,828 - -0.2 430,828 - -0.2 430,829 + -0.1 430,829 + +0.3 430,846 + -0.5 430,878 + -1.0 430,901 - +0.5 430,902 + +0.6 430,964 - +0.3 430,992 + +0.8 430,992 + -0.9 431,073 - MMJJ_RS02280 0.15 +1.2 431,074 + MMJJ_RS02280 0.15 -0.0 431,126 - MMJJ_RS02280 0.18 +0.4 431,160 - MMJJ_RS02280 0.20 -0.2 431,196 - MMJJ_RS02280 0.22 +0.2 431,197 + MMJJ_RS02280 0.22 +0.2 431,217 + MMJJ_RS02280 0.23 +0.4 431,295 - MMJJ_RS02280 0.28 +0.7 431,295 - MMJJ_RS02280 0.28 -0.8 431,299 - MMJJ_RS02280 0.28 -0.1 431,300 + MMJJ_RS02280 0.28 -0.7 431,300 + MMJJ_RS02280 0.28 -0.2 431,300 + MMJJ_RS02280 0.28 +0.0 431,300 + MMJJ_RS02280 0.28 +1.2 431,310 - MMJJ_RS02280 0.29 -0.1 431,321 - MMJJ_RS02280 0.30 -0.2 431,321 - MMJJ_RS02280 0.30 +0.4 431,321 - MMJJ_RS02280 0.30 +1.8 431,322 + MMJJ_RS02280 0.30 -0.6 431,358 + MMJJ_RS02280 0.32 -0.3 431,362 + MMJJ_RS02280 0.32 +0.4 431,408 - MMJJ_RS02280 0.35 -0.1 431,417 - MMJJ_RS02280 0.35 -0.4 431,442 - MMJJ_RS02280 0.37 +0.1 431,443 + MMJJ_RS02280 0.37 -0.4 431,443 + -1.6 431,443 + MMJJ_RS02280 0.37 +0.4 431,454 + MMJJ_RS02280 0.38 +0.0 431,476 + MMJJ_RS02280 0.39 -1.0 431,568 - MMJJ_RS02280 0.44 -1.0 431,588 - MMJJ_RS02280 0.46 -1.0
Or see this region's nucleotide sequence