Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01860 and MMJJ_RS01865 are separated by 147 nucleotides MMJJ_RS01865 and MMJJ_RS01870 are separated by 135 nucleotides MMJJ_RS01870 and MMJJ_RS01875 are separated by 36 nucleotides
MMJJ_RS01860: MMJJ_RS01860 - tRNA guanosine(15) transglycosylase TgtA, at 356,203 to 358,152
_RS01860
MMJJ_RS01865: MMJJ_RS01865 - peptidyl-tRNA hydrolase Pth2, at 358,300 to 358,650
_RS01865
MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021
_RS01870
MMJJ_RS01875: MMJJ_RS01875 - global nitrogen regulator NrpR, at 360,058 to 361,668
_RS01875
Position (kb) 358
359
360
361 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.971 kb on - strand at 357.972 kb on + strand at 357.977 kb on + strand at 357.977 kb on + strand at 357.995 kb on + strand at 358.019 kb on + strand at 358.060 kb on - strand at 358.109 kb on + strand at 358.120 kb on + strand at 358.163 kb on - strand at 358.164 kb on + strand at 358.164 kb on + strand at 358.168 kb on - strand at 358.168 kb on - strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.177 kb on - strand at 358.177 kb on - strand at 358.178 kb on + strand at 358.184 kb on - strand at 358.184 kb on - strand at 358.207 kb on - strand at 358.207 kb on - strand at 358.220 kb on - strand at 358.225 kb on - strand at 358.226 kb on + strand at 358.232 kb on - strand at 358.232 kb on - strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.236 kb on - strand at 358.236 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.240 kb on + strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.245 kb on - strand at 358.245 kb on - strand at 358.246 kb on + strand at 358.246 kb on + strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.249 kb on + strand at 358.249 kb on + strand at 358.255 kb on - strand at 358.255 kb on - strand at 358.256 kb on + strand at 358.258 kb on + strand at 358.320 kb on - strand at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.470 kb on + strand, within MMJJ_RS01865 at 358.497 kb on + strand, within MMJJ_RS01865 at 358.535 kb on - strand, within MMJJ_RS01865 at 358.536 kb on + strand, within MMJJ_RS01865 at 358.648 kb on - strand at 358.649 kb on + strand at 358.656 kb on + strand at 358.672 kb on - strand at 358.690 kb on + strand at 358.690 kb on + strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.693 kb on + strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.695 kb on + strand at 358.699 kb on + strand at 358.716 kb on - strand at 358.722 kb on + strand at 358.722 kb on + strand at 358.883 kb on - strand at 359.061 kb on + strand at 359.082 kb on + strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.733 kb on + strand, within MMJJ_RS01870 at 359.855 kb on - strand, within MMJJ_RS01870 at 359.856 kb on + strand at 359.965 kb on + strand at 360.045 kb on - strand at 360.045 kb on - strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.046 kb on + strand at 360.158 kb on + strand at 360.191 kb on - strand at 360.294 kb on - strand, within MMJJ_RS01875 at 360.414 kb on + strand, within MMJJ_RS01875 at 360.437 kb on + strand at 360.477 kb on - strand, within MMJJ_RS01875 at 360.477 kb on - strand, within MMJJ_RS01875 at 360.478 kb on + strand, within MMJJ_RS01875 at 360.488 kb on - strand, within MMJJ_RS01875 at 360.501 kb on - strand, within MMJJ_RS01875 at 360.501 kb on - strand, within MMJJ_RS01875 at 360.540 kb on + strand, within MMJJ_RS01875 at 360.540 kb on + strand, within MMJJ_RS01875 at 360.651 kb on - strand, within MMJJ_RS01875 at 360.675 kb on + strand, within MMJJ_RS01875 at 360.900 kb on - strand, within MMJJ_RS01875 at 360.911 kb on - strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875 at 360.912 kb on + strand, within MMJJ_RS01875
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 357,854 + MMJJ_RS01860 0.85 -1.6 357,854 + MMJJ_RS01860 0.85 -2.4 357,971 - -3.6 357,972 + -1.3 357,977 + -2.8 357,977 + -1.5 357,995 + -0.5 358,019 + -1.8 358,060 - -2.5 358,109 + -3.9 358,120 + -3.7 358,163 - -0.2 358,164 + +0.1 358,164 + -1.3 358,168 - -2.0 358,168 - +0.4 358,176 + -0.7 358,176 + +0.7 358,176 + +0.6 358,177 - -2.0 358,177 - -0.6 358,178 + -0.2 358,184 - +0.5 358,184 - +0.4 358,207 - +0.2 358,207 - +0.0 358,220 - -0.5 358,225 - -1.5 358,226 + +1.0 358,232 - -1.9 358,232 - -0.2 358,233 + -1.2 358,233 + -1.0 358,233 + -1.2 358,233 + +0.3 358,236 - +0.8 358,236 - +0.4 358,239 - -0.1 358,239 - -0.5 358,239 - +0.9 358,240 + -2.7 358,243 - -0.7 358,243 - -0.6 358,243 - +0.9 358,243 - -0.7 358,243 - +0.3 358,244 + +0.7 358,244 + +0.9 358,244 + -0.9 358,244 + +0.5 358,244 + +0.3 358,244 + -0.9 358,245 - -0.1 358,245 - -0.5 358,246 + -1.1 358,246 + -1.2 358,248 - -0.8 358,248 - -2.6 358,248 - -3.2 358,249 + +0.5 358,249 + -0.4 358,255 - -1.0 358,255 - -0.6 358,256 + -0.4 358,258 + -1.5 358,320 - -0.4 358,335 + MMJJ_RS01865 0.10 -0.6 358,335 + MMJJ_RS01865 0.10 -2.0 358,335 + MMJJ_RS01865 0.10 -1.0 358,410 + MMJJ_RS01865 0.31 -1.0 358,410 + MMJJ_RS01865 0.31 -0.9 358,470 + MMJJ_RS01865 0.48 +0.2 358,497 + MMJJ_RS01865 0.56 -0.7 358,535 - MMJJ_RS01865 0.67 -1.7 358,536 + MMJJ_RS01865 0.67 -2.9 358,648 - -1.3 358,649 + +0.3 358,656 + -0.4 358,672 - +0.5 358,690 + +0.6 358,690 + -0.9 358,692 - -1.0 358,692 - -2.2 358,692 - -0.6 358,693 + +1.0 358,694 - +0.4 358,694 - +0.3 358,694 - +0.2 358,695 + +1.2 358,699 + +0.1 358,716 - -2.6 358,722 + -1.6 358,722 + -3.3 358,883 - -2.2 359,061 + +0.0 359,082 + MMJJ_RS01870 0.24 -2.4 359,528 - MMJJ_RS01870 0.60 -1.3 359,528 - MMJJ_RS01870 0.60 -1.2 359,733 + MMJJ_RS01870 0.77 -2.3 359,855 - MMJJ_RS01870 0.86 -0.6 359,856 + -1.6 359,965 + -1.0 360,045 - +0.0 360,045 - -0.6 360,046 + -2.0 360,046 + -0.6 360,046 + -3.0 360,046 + -1.6 360,046 + -3.3 360,158 + -0.7 360,191 - -2.8 360,294 - MMJJ_RS01875 0.15 -1.7 360,414 + MMJJ_RS01875 0.22 -0.9 360,437 + +0.0 360,477 - MMJJ_RS01875 0.26 -1.2 360,477 - MMJJ_RS01875 0.26 -1.1 360,478 + MMJJ_RS01875 0.26 -0.3 360,488 - MMJJ_RS01875 0.27 -1.0 360,501 - MMJJ_RS01875 0.27 -0.6 360,501 - MMJJ_RS01875 0.27 -0.8 360,540 + MMJJ_RS01875 0.30 +0.4 360,540 + MMJJ_RS01875 0.30 -1.6 360,651 - MMJJ_RS01875 0.37 -1.9 360,675 + MMJJ_RS01875 0.38 +0.0 360,900 - MMJJ_RS01875 0.52 -1.7 360,911 - MMJJ_RS01875 0.53 -1.9 360,912 + MMJJ_RS01875 0.53 -2.6 360,912 + MMJJ_RS01875 0.53 -1.8
Or see this region's nucleotide sequence