Experiment: PYE with Carbenicillin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00421 and CCNA_00422 are separated by 49 nucleotides CCNA_00422 and CCNA_03919 are separated by 1 nucleotides CCNA_03919 and CCNA_00423 are separated by 233 nucleotides CCNA_00423 and CCNA_00424 are separated by 246 nucleotides
CCNA_00421: CCNA_00421 - dihydroneopterin aldolase, at 431,882 to 432,286
_00421
CCNA_00422: CCNA_00422 - SDR pteridine reductase, at 432,336 to 433,088
_00422
CCNA_03919: CCNA_03919 - hypothetical protein, at 433,090 to 433,170
_03919
CCNA_00423: CCNA_00423 - phosphohydrolase, MutT-nudix family, at 433,404 to 433,874
_00423
CCNA_00424: CCNA_00424 - aldehyde dehydrogenase, at 434,121 to 435,560
_00424
Position (kb) 433
434 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 432.309 kb on + strand at 432.314 kb on + strand at 432.322 kb on + strand at 432.330 kb on - strand at 432.333 kb on + strand at 432.341 kb on - strand at 432.344 kb on - strand at 432.374 kb on - strand at 432.396 kb on + strand at 432.404 kb on - strand at 432.435 kb on - strand, within CCNA_00422 at 432.452 kb on + strand, within CCNA_00422 at 432.460 kb on - strand, within CCNA_00422 at 432.533 kb on + strand, within CCNA_00422 at 432.536 kb on - strand, within CCNA_00422 at 432.545 kb on + strand, within CCNA_00422 at 432.587 kb on - strand, within CCNA_00422 at 432.588 kb on - strand at 432.591 kb on + strand, within CCNA_00422 at 432.591 kb on + strand, within CCNA_00422 at 432.598 kb on - strand, within CCNA_00422 at 432.616 kb on + strand, within CCNA_00422 at 432.621 kb on + strand, within CCNA_00422 at 432.626 kb on + strand, within CCNA_00422 at 432.724 kb on + strand, within CCNA_00422 at 432.732 kb on - strand, within CCNA_00422 at 432.760 kb on - strand, within CCNA_00422 at 432.793 kb on + strand, within CCNA_00422 at 432.824 kb on - strand, within CCNA_00422 at 432.903 kb on - strand, within CCNA_00422 at 432.920 kb on - strand, within CCNA_00422 at 432.944 kb on - strand, within CCNA_00422 at 432.963 kb on + strand, within CCNA_00422 at 432.984 kb on + strand, within CCNA_00422 at 433.007 kb on - strand, within CCNA_00422 at 433.039 kb on - strand at 433.062 kb on + strand at 433.067 kb on + strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.070 kb on - strand at 433.075 kb on - strand at 433.102 kb on + strand, within CCNA_03919 at 433.116 kb on + strand, within CCNA_03919 at 433.124 kb on - strand, within CCNA_03919 at 433.125 kb on - strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.136 kb on + strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.144 kb on - strand, within CCNA_03919 at 433.148 kb on - strand, within CCNA_03919 at 433.172 kb on + strand at 433.205 kb on - strand at 433.233 kb on - strand at 433.245 kb on + strand at 433.253 kb on - strand at 433.264 kb on + strand at 433.268 kb on - strand at 433.268 kb on - strand at 433.304 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.306 kb on + strand at 433.319 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.338 kb on + strand at 433.346 kb on - strand at 433.346 kb on - strand at 433.378 kb on + strand at 433.390 kb on + strand at 433.390 kb on + strand at 433.397 kb on - strand at 433.406 kb on + strand at 433.406 kb on + strand at 433.414 kb on - strand at 433.445 kb on + strand at 433.445 kb on + strand at 433.452 kb on + strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.453 kb on - strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.474 kb on + strand, within CCNA_00423 at 433.482 kb on - strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.514 kb on + strand, within CCNA_00423 at 433.522 kb on - strand, within CCNA_00423 at 433.538 kb on + strand, within CCNA_00423 at 433.541 kb on - strand, within CCNA_00423 at 433.546 kb on - strand, within CCNA_00423 at 433.552 kb on - strand, within CCNA_00423 at 433.559 kb on - strand, within CCNA_00423 at 433.560 kb on - strand, within CCNA_00423 at 433.668 kb on + strand, within CCNA_00423 at 433.683 kb on + strand, within CCNA_00423 at 433.696 kb on - strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.707 kb on + strand, within CCNA_00423 at 433.741 kb on + strand, within CCNA_00423 at 433.749 kb on - strand, within CCNA_00423 at 433.815 kb on - strand, within CCNA_00423 at 433.839 kb on + strand at 433.844 kb on + strand at 433.846 kb on + strand at 433.851 kb on + strand at 433.851 kb on + strand at 433.855 kb on + strand at 433.859 kb on - strand at 433.863 kb on - strand at 433.870 kb on + strand at 433.873 kb on - strand at 433.875 kb on + strand at 433.878 kb on + strand at 433.899 kb on - strand at 433.915 kb on - strand at 433.925 kb on - strand at 433.926 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.949 kb on + strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.957 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.965 kb on - strand at 433.996 kb on + strand at 433.997 kb on - strand at 434.033 kb on + strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.041 kb on - strand at 434.060 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.105 kb on + strand at 434.113 kb on - strand at 434.113 kb on - strand at 434.113 kb on - strand at 434.128 kb on + strand at 434.138 kb on + strand at 434.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Carbenicillin 0.25 mg/ml remove 432,309 + +1.4 432,314 + +0.1 432,322 + +0.3 432,330 - +1.9 432,333 + +0.3 432,341 - +1.2 432,344 - +0.4 432,374 - -3.1 432,396 + -0.6 432,404 - -1.4 432,435 - CCNA_00422 0.13 -1.0 432,452 + CCNA_00422 0.15 -2.0 432,460 - CCNA_00422 0.16 -1.3 432,533 + CCNA_00422 0.26 -1.3 432,536 - CCNA_00422 0.27 -3.0 432,545 + CCNA_00422 0.28 -0.0 432,587 - CCNA_00422 0.33 -0.6 432,588 - +1.5 432,591 + CCNA_00422 0.34 -0.5 432,591 + CCNA_00422 0.34 -2.1 432,598 - CCNA_00422 0.35 +0.2 432,616 + CCNA_00422 0.37 +0.3 432,621 + CCNA_00422 0.38 -1.3 432,626 + CCNA_00422 0.39 -1.2 432,724 + CCNA_00422 0.52 -0.3 432,732 - CCNA_00422 0.53 +0.4 432,760 - CCNA_00422 0.56 -0.7 432,793 + CCNA_00422 0.61 +0.8 432,824 - CCNA_00422 0.65 -1.5 432,903 - CCNA_00422 0.75 -1.2 432,920 - CCNA_00422 0.78 -1.2 432,944 - CCNA_00422 0.81 -0.5 432,963 + CCNA_00422 0.83 +1.1 432,984 + CCNA_00422 0.86 -0.5 433,007 - CCNA_00422 0.89 -1.9 433,039 - -1.7 433,062 + -0.7 433,067 + -0.5 433,070 - -2.1 433,070 - -4.1 433,070 - -0.9 433,070 - -0.8 433,075 - -1.3 433,102 + CCNA_03919 0.15 +0.1 433,116 + CCNA_03919 0.32 +0.5 433,124 - CCNA_03919 0.42 +3.0 433,125 - CCNA_03919 0.43 -2.2 433,136 + CCNA_03919 0.57 -0.3 433,136 + CCNA_03919 0.57 -2.8 433,144 - CCNA_03919 0.67 +2.6 433,144 - CCNA_03919 0.67 +0.7 433,148 - CCNA_03919 0.72 +1.1 433,172 + +0.4 433,205 - -0.5 433,233 - -0.6 433,245 + +1.8 433,253 - -1.0 433,264 + +0.3 433,268 - -0.9 433,268 - -1.9 433,304 + -0.5 433,306 + -0.2 433,306 + +0.3 433,306 + -1.6 433,306 + -1.3 433,306 + +0.6 433,319 + -0.3 433,338 + -0.6 433,338 + -0.9 433,338 + +1.2 433,338 + -2.6 433,346 - +1.1 433,346 - -0.3 433,378 + -0.5 433,390 + +1.8 433,390 + +0.1 433,397 - -1.3 433,406 + -2.4 433,406 + +0.8 433,414 - -1.7 433,445 + -1.6 433,445 + -1.9 433,452 + CCNA_00423 0.10 -0.7 433,453 - CCNA_00423 0.10 -2.3 433,453 - CCNA_00423 0.10 -1.4 433,474 + CCNA_00423 0.15 +0.0 433,474 + CCNA_00423 0.15 -0.7 433,474 + CCNA_00423 0.15 -0.7 433,474 + CCNA_00423 0.15 -3.3 433,474 + CCNA_00423 0.15 +1.5 433,474 + CCNA_00423 0.15 +0.1 433,474 + CCNA_00423 0.15 +0.1 433,482 - CCNA_00423 0.17 -0.0 433,514 + CCNA_00423 0.23 -1.1 433,514 + CCNA_00423 0.23 -0.1 433,522 - CCNA_00423 0.25 -0.5 433,538 + CCNA_00423 0.28 -1.1 433,541 - CCNA_00423 0.29 +0.6 433,546 - CCNA_00423 0.30 +1.0 433,552 - CCNA_00423 0.31 -2.7 433,559 - CCNA_00423 0.33 -0.5 433,560 - CCNA_00423 0.33 -0.3 433,668 + CCNA_00423 0.56 -0.7 433,683 + CCNA_00423 0.59 +0.8 433,696 - CCNA_00423 0.62 -1.3 433,707 + CCNA_00423 0.64 +0.5 433,707 + CCNA_00423 0.64 +0.2 433,741 + CCNA_00423 0.72 -2.0 433,749 - CCNA_00423 0.73 -0.7 433,815 - CCNA_00423 0.87 -0.1 433,839 + +0.0 433,844 + +0.5 433,846 + -0.3 433,851 + +0.5 433,851 + +0.1 433,855 + -0.7 433,859 - -1.6 433,863 - +0.1 433,870 + -1.8 433,873 - -0.6 433,875 + +0.3 433,878 + +0.7 433,899 - +1.2 433,915 - +0.2 433,925 - -1.2 433,926 + +0.7 433,949 + -1.0 433,949 + -1.5 433,949 + -0.0 433,949 + +0.2 433,949 + +1.0 433,949 + -1.9 433,957 - -0.5 433,957 - -1.8 433,957 - -0.0 433,957 - -0.3 433,957 - +1.5 433,957 - -0.7 433,965 - +0.2 433,965 - +2.7 433,965 - -0.6 433,996 + +1.1 433,997 - -1.5 434,033 + +2.3 434,041 - -1.3 434,041 - -0.2 434,041 - +0.4 434,060 + -0.4 434,105 + +0.0 434,105 + +0.3 434,105 + -2.4 434,105 + -0.5 434,105 + +0.2 434,113 - -0.2 434,113 - +2.1 434,113 - +1.6 434,128 + -0.8 434,138 + -1.8 434,146 - -0.3
Or see this region's nucleotide sequence