Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31255 and AZOBR_RS31260 are separated by 111 nucleotides AZOBR_RS31260 and AZOBR_RS31265 are separated by 17 nucleotides
AZOBR_RS31255: AZOBR_RS31255 - galactose 1-dehydrogenase, at 377,161 to 378,138
_RS31255
AZOBR_RS31260: AZOBR_RS31260 - dihydroxy-acid dehydratase, at 378,250 to 380,076
_RS31260
AZOBR_RS31265: AZOBR_RS31265 - lysophospholipase, at 380,094 to 381,314
_RS31265
Position (kb) 378
379
380
381 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 377.923 kb on - strand, within AZOBR_RS31255 at 378.004 kb on + strand, within AZOBR_RS31255 at 378.004 kb on + strand, within AZOBR_RS31255 at 378.016 kb on + strand, within AZOBR_RS31255 at 378.017 kb on - strand, within AZOBR_RS31255 at 378.136 kb on + strand at 378.136 kb on + strand at 378.359 kb on - strand at 378.359 kb on - strand at 378.359 kb on - strand at 378.371 kb on - strand at 378.994 kb on + strand, within AZOBR_RS31260 at 378.995 kb on - strand, within AZOBR_RS31260 at 378.995 kb on - strand, within AZOBR_RS31260 at 379.234 kb on + strand, within AZOBR_RS31260 at 379.504 kb on + strand, within AZOBR_RS31260 at 379.504 kb on + strand, within AZOBR_RS31260 at 379.505 kb on - strand, within AZOBR_RS31260 at 379.505 kb on - strand, within AZOBR_RS31260 at 379.505 kb on - strand, within AZOBR_RS31260 at 379.505 kb on - strand, within AZOBR_RS31260 at 379.573 kb on + strand, within AZOBR_RS31260 at 379.574 kb on - strand, within AZOBR_RS31260 at 379.574 kb on - strand, within AZOBR_RS31260 at 379.615 kb on + strand, within AZOBR_RS31260 at 379.615 kb on + strand, within AZOBR_RS31260 at 379.615 kb on + strand, within AZOBR_RS31260 at 379.616 kb on - strand, within AZOBR_RS31260 at 380.029 kb on + strand at 380.030 kb on - strand at 380.350 kb on + strand, within AZOBR_RS31265 at 380.351 kb on - strand, within AZOBR_RS31265 at 380.351 kb on - strand, within AZOBR_RS31265 at 380.738 kb on - strand, within AZOBR_RS31265 at 380.738 kb on - strand, within AZOBR_RS31265 at 380.783 kb on - strand, within AZOBR_RS31265 at 380.818 kb on + strand, within AZOBR_RS31265 at 380.818 kb on + strand, within AZOBR_RS31265 at 380.818 kb on + strand, within AZOBR_RS31265 at 380.818 kb on + strand, within AZOBR_RS31265 at 380.818 kb on + strand, within AZOBR_RS31265 at 380.819 kb on - strand, within AZOBR_RS31265 at 380.819 kb on - strand, within AZOBR_RS31265 at 380.819 kb on - strand, within AZOBR_RS31265 at 381.040 kb on + strand, within AZOBR_RS31265
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 377,923 - AZOBR_RS31255 0.78 -0.3 378,004 + AZOBR_RS31255 0.86 -1.3 378,004 + AZOBR_RS31255 0.86 -0.0 378,016 + AZOBR_RS31255 0.87 -0.1 378,017 - AZOBR_RS31255 0.88 +0.7 378,136 + -1.8 378,136 + -0.6 378,359 - -0.3 378,359 - -0.4 378,359 - +0.4 378,371 - -0.2 378,994 + AZOBR_RS31260 0.41 -0.2 378,995 - AZOBR_RS31260 0.41 +3.2 378,995 - AZOBR_RS31260 0.41 +0.8 379,234 + AZOBR_RS31260 0.54 -0.3 379,504 + AZOBR_RS31260 0.69 -2.4 379,504 + AZOBR_RS31260 0.69 -3.3 379,505 - AZOBR_RS31260 0.69 +0.9 379,505 - AZOBR_RS31260 0.69 +0.8 379,505 - AZOBR_RS31260 0.69 +0.1 379,505 - AZOBR_RS31260 0.69 -2.1 379,573 + AZOBR_RS31260 0.72 +0.2 379,574 - AZOBR_RS31260 0.72 +0.0 379,574 - AZOBR_RS31260 0.72 +0.6 379,615 + AZOBR_RS31260 0.75 +1.6 379,615 + AZOBR_RS31260 0.75 +0.1 379,615 + AZOBR_RS31260 0.75 -1.1 379,616 - AZOBR_RS31260 0.75 -2.0 380,029 + +2.0 380,030 - -1.6 380,350 + AZOBR_RS31265 0.21 +2.0 380,351 - AZOBR_RS31265 0.21 +0.0 380,351 - AZOBR_RS31265 0.21 -3.0 380,738 - AZOBR_RS31265 0.53 +1.9 380,738 - AZOBR_RS31265 0.53 +0.8 380,783 - AZOBR_RS31265 0.56 -0.8 380,818 + AZOBR_RS31265 0.59 -0.6 380,818 + AZOBR_RS31265 0.59 -1.1 380,818 + AZOBR_RS31265 0.59 -2.1 380,818 + AZOBR_RS31265 0.59 -0.6 380,818 + AZOBR_RS31265 0.59 -1.9 380,819 - AZOBR_RS31265 0.59 +0.0 380,819 - AZOBR_RS31265 0.59 -1.3 380,819 - AZOBR_RS31265 0.59 -0.7 381,040 + AZOBR_RS31265 0.77 -0.4
Or see this region's nucleotide sequence