Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31085 and AZOBR_RS31090 are separated by 196 nucleotides AZOBR_RS31090 and AZOBR_RS31095 are separated by 3 nucleotides AZOBR_RS31095 and AZOBR_RS31100 are separated by 237 nucleotides AZOBR_RS31100 and AZOBR_RS31105 are separated by 133 nucleotides
AZOBR_RS31085: AZOBR_RS31085 - hypothetical protein, at 339,006 to 339,752
_RS31085
AZOBR_RS31090: AZOBR_RS31090 - pseudouridine synthase, at 339,949 to 340,659
_RS31090
AZOBR_RS31095: AZOBR_RS31095 - hypothetical protein, at 340,663 to 341,670
_RS31095
AZOBR_RS31100: AZOBR_RS31100 - endonuclease III, at 341,908 to 342,300
_RS31100
AZOBR_RS31105: AZOBR_RS31105 - 8-amino-7-oxononanoate synthase, at 342,434 to 343,615
_RS31105
Position (kb) 340
341
342 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 340.243 kb on + strand, within AZOBR_RS31090 at 340.244 kb on - strand at 340.372 kb on + strand, within AZOBR_RS31090 at 340.372 kb on + strand, within AZOBR_RS31090 at 340.373 kb on - strand, within AZOBR_RS31090 at 340.492 kb on + strand, within AZOBR_RS31090 at 340.492 kb on + strand, within AZOBR_RS31090 at 340.493 kb on - strand, within AZOBR_RS31090 at 340.495 kb on + strand, within AZOBR_RS31090 at 340.495 kb on + strand, within AZOBR_RS31090 at 340.657 kb on + strand at 340.657 kb on + strand at 340.964 kb on + strand, within AZOBR_RS31095 at 340.964 kb on + strand, within AZOBR_RS31095 at 340.965 kb on - strand, within AZOBR_RS31095 at 341.310 kb on - strand, within AZOBR_RS31095 at 341.352 kb on - strand at 341.352 kb on - strand, within AZOBR_RS31095 at 341.652 kb on - strand at 341.839 kb on + strand at 341.839 kb on + strand at 341.839 kb on + strand at 341.839 kb on + strand at 341.839 kb on + strand at 341.839 kb on + strand at 341.840 kb on - strand at 341.840 kb on - strand at 341.840 kb on - strand at 341.840 kb on - strand at 342.032 kb on - strand, within AZOBR_RS31100 at 342.191 kb on + strand, within AZOBR_RS31100 at 342.198 kb on - strand, within AZOBR_RS31100 at 342.278 kb on + strand at 342.278 kb on + strand at 342.278 kb on + strand at 342.278 kb on + strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.279 kb on - strand at 342.354 kb on - strand at 342.354 kb on - strand at 342.385 kb on + strand at 342.419 kb on + strand at 342.582 kb on - strand, within AZOBR_RS31105
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 340,243 + AZOBR_RS31090 0.41 -3.3 340,244 - +0.4 340,372 + AZOBR_RS31090 0.59 -0.2 340,372 + AZOBR_RS31090 0.59 -1.0 340,373 - AZOBR_RS31090 0.60 -2.6 340,492 + AZOBR_RS31090 0.76 +1.0 340,492 + AZOBR_RS31090 0.76 +0.1 340,493 - AZOBR_RS31090 0.77 +1.0 340,495 + AZOBR_RS31090 0.77 -1.6 340,495 + AZOBR_RS31090 0.77 -1.0 340,657 + -0.6 340,657 + -3.3 340,964 + AZOBR_RS31095 0.30 -0.2 340,964 + AZOBR_RS31095 0.30 +0.0 340,965 - AZOBR_RS31095 0.30 +0.6 341,310 - AZOBR_RS31095 0.64 +0.7 341,352 - +0.4 341,352 - AZOBR_RS31095 0.68 +0.8 341,652 - +1.3 341,839 + -0.2 341,839 + -3.0 341,839 + +1.3 341,839 + +1.7 341,839 + -1.3 341,839 + +0.1 341,840 - -3.8 341,840 - -2.1 341,840 - -3.1 341,840 - -1.7 342,032 - AZOBR_RS31100 0.32 +1.7 342,191 + AZOBR_RS31100 0.72 -0.2 342,198 - AZOBR_RS31100 0.74 +1.2 342,278 + +0.9 342,278 + -2.0 342,278 + -3.2 342,278 + -3.6 342,279 - +0.3 342,279 - +0.5 342,279 - -0.5 342,279 - +1.3 342,279 - +0.3 342,279 - -2.3 342,279 - -0.6 342,354 - +1.5 342,354 - -0.1 342,385 + +3.0 342,419 + +0.2 342,582 - AZOBR_RS31105 0.13 +0.6
Or see this region's nucleotide sequence