Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30515 and AZOBR_RS30520 are separated by 279 nucleotides AZOBR_RS30520 and AZOBR_RS30525 are separated by 16 nucleotides
AZOBR_RS30515: AZOBR_RS30515 - phosphoenolpyruvate carboxykinase, at 193,052 to 194,875
_RS30515
AZOBR_RS30520: AZOBR_RS30520 - flagellar motor protein MotA, at 195,155 to 196,015
_RS30520
AZOBR_RS30525: AZOBR_RS30525 - hypothetical protein, at 196,032 to 197,018
_RS30525
Position (kb) 195
196
197 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 194.327 kb on + strand, within AZOBR_RS30515 at 194.327 kb on + strand, within AZOBR_RS30515 at 194.327 kb on + strand, within AZOBR_RS30515 at 194.328 kb on - strand, within AZOBR_RS30515 at 194.348 kb on + strand, within AZOBR_RS30515 at 194.349 kb on - strand, within AZOBR_RS30515 at 194.444 kb on + strand, within AZOBR_RS30515 at 194.444 kb on + strand, within AZOBR_RS30515 at 194.445 kb on - strand, within AZOBR_RS30515 at 194.459 kb on + strand, within AZOBR_RS30515 at 194.459 kb on + strand, within AZOBR_RS30515 at 194.513 kb on + strand, within AZOBR_RS30515 at 194.513 kb on + strand, within AZOBR_RS30515 at 195.007 kb on + strand at 195.008 kb on - strand at 195.035 kb on - strand at 195.207 kb on - strand at 195.523 kb on - strand, within AZOBR_RS30520 at 195.539 kb on + strand, within AZOBR_RS30520 at 195.539 kb on + strand, within AZOBR_RS30520 at 195.539 kb on + strand, within AZOBR_RS30520 at 195.803 kb on + strand, within AZOBR_RS30520 at 195.804 kb on - strand, within AZOBR_RS30520 at 195.845 kb on + strand, within AZOBR_RS30520 at 195.846 kb on - strand, within AZOBR_RS30520 at 195.863 kb on + strand, within AZOBR_RS30520 at 195.863 kb on + strand, within AZOBR_RS30520 at 195.864 kb on - strand, within AZOBR_RS30520 at 195.864 kb on - strand, within AZOBR_RS30520 at 195.867 kb on - strand, within AZOBR_RS30520 at 196.791 kb on + strand, within AZOBR_RS30525 at 196.791 kb on + strand, within AZOBR_RS30525 at 196.815 kb on + strand, within AZOBR_RS30525 at 196.816 kb on - strand, within AZOBR_RS30525 at 196.816 kb on - strand, within AZOBR_RS30525 at 196.816 kb on - strand, within AZOBR_RS30525
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 194,327 + AZOBR_RS30515 0.70 +1.8 194,327 + AZOBR_RS30515 0.70 +0.9 194,327 + AZOBR_RS30515 0.70 +1.4 194,328 - AZOBR_RS30515 0.70 +1.3 194,348 + AZOBR_RS30515 0.71 +1.0 194,349 - AZOBR_RS30515 0.71 +1.0 194,444 + AZOBR_RS30515 0.76 +1.7 194,444 + AZOBR_RS30515 0.76 +0.2 194,445 - AZOBR_RS30515 0.76 +1.3 194,459 + AZOBR_RS30515 0.77 +0.7 194,459 + AZOBR_RS30515 0.77 +1.6 194,513 + AZOBR_RS30515 0.80 +1.3 194,513 + AZOBR_RS30515 0.80 +1.3 195,007 + -2.8 195,008 - -2.1 195,035 - -1.3 195,207 - -5.2 195,523 - AZOBR_RS30520 0.43 -4.5 195,539 + AZOBR_RS30520 0.45 -6.0 195,539 + AZOBR_RS30520 0.45 -0.9 195,539 + AZOBR_RS30520 0.45 -1.7 195,803 + AZOBR_RS30520 0.75 -4.4 195,804 - AZOBR_RS30520 0.75 -4.0 195,845 + AZOBR_RS30520 0.80 -3.8 195,846 - AZOBR_RS30520 0.80 -2.3 195,863 + AZOBR_RS30520 0.82 -2.0 195,863 + AZOBR_RS30520 0.82 -2.0 195,864 - AZOBR_RS30520 0.82 -3.6 195,864 - AZOBR_RS30520 0.82 -1.3 195,867 - AZOBR_RS30520 0.83 -3.6 196,791 + AZOBR_RS30525 0.77 -1.0 196,791 + AZOBR_RS30525 0.77 +1.2 196,815 + AZOBR_RS30525 0.79 +0.0 196,816 - AZOBR_RS30525 0.79 -2.0 196,816 - AZOBR_RS30525 0.79 -0.7 196,816 - AZOBR_RS30525 0.79 +3.9
Or see this region's nucleotide sequence