Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS28325 and AZOBR_RS28330 are separated by 269 nucleotides AZOBR_RS28330 and AZOBR_RS28335 are separated by 202 nucleotides
AZOBR_RS28325: AZOBR_RS28325 - DEAD/DEAH box helicase, at 525,362 to 527,164
_RS28325
AZOBR_RS28330: AZOBR_RS28330 - potassium channel protein, at 527,434 to 528,516
_RS28330
AZOBR_RS28335: AZOBR_RS28335 - hypothetical protein, at 528,719 to 530,371
_RS28335
Position (kb) 527
528
529 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 526.664 kb on + strand, within AZOBR_RS28325 at 526.664 kb on + strand, within AZOBR_RS28325 at 526.664 kb on + strand, within AZOBR_RS28325 at 526.664 kb on + strand, within AZOBR_RS28325 at 526.664 kb on + strand, within AZOBR_RS28325 at 526.665 kb on - strand, within AZOBR_RS28325 at 526.665 kb on - strand, within AZOBR_RS28325 at 526.665 kb on - strand, within AZOBR_RS28325 at 526.670 kb on + strand, within AZOBR_RS28325 at 526.857 kb on - strand, within AZOBR_RS28325 at 526.857 kb on - strand, within AZOBR_RS28325 at 526.949 kb on + strand, within AZOBR_RS28325 at 527.772 kb on - strand, within AZOBR_RS28330 at 527.772 kb on - strand, within AZOBR_RS28330 at 527.868 kb on + strand, within AZOBR_RS28330 at 527.868 kb on + strand, within AZOBR_RS28330 at 527.869 kb on - strand, within AZOBR_RS28330 at 528.241 kb on + strand, within AZOBR_RS28330 at 528.242 kb on - strand, within AZOBR_RS28330 at 528.444 kb on + strand at 528.444 kb on + strand at 528.515 kb on - strand at 528.619 kb on - strand at 528.684 kb on + strand at 528.684 kb on + strand at 528.684 kb on + strand at 528.684 kb on + strand at 528.756 kb on + strand at 529.168 kb on - strand, within AZOBR_RS28335 at 529.168 kb on - strand, within AZOBR_RS28335 at 529.168 kb on - strand, within AZOBR_RS28335 at 529.168 kb on - strand, within AZOBR_RS28335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 526,664 + AZOBR_RS28325 0.72 +1.0 526,664 + AZOBR_RS28325 0.72 +0.9 526,664 + AZOBR_RS28325 0.72 -4.0 526,664 + AZOBR_RS28325 0.72 -1.9 526,664 + AZOBR_RS28325 0.72 -1.1 526,665 - AZOBR_RS28325 0.72 -3.6 526,665 - AZOBR_RS28325 0.72 -0.7 526,665 - AZOBR_RS28325 0.72 -3.0 526,670 + AZOBR_RS28325 0.73 -0.7 526,857 - AZOBR_RS28325 0.83 -0.7 526,857 - AZOBR_RS28325 0.83 -2.4 526,949 + AZOBR_RS28325 0.88 +1.5 527,772 - AZOBR_RS28330 0.31 -0.6 527,772 - AZOBR_RS28330 0.31 -3.2 527,868 + AZOBR_RS28330 0.40 -2.1 527,868 + AZOBR_RS28330 0.40 -1.3 527,869 - AZOBR_RS28330 0.40 -0.4 528,241 + AZOBR_RS28330 0.75 +0.1 528,242 - AZOBR_RS28330 0.75 -1.7 528,444 + +1.5 528,444 + -2.0 528,515 - +0.6 528,619 - +0.3 528,684 + +0.1 528,684 + -1.6 528,684 + +2.3 528,684 + +0.1 528,756 + +0.8 529,168 - AZOBR_RS28335 0.27 +1.3 529,168 - AZOBR_RS28335 0.27 +0.7 529,168 - AZOBR_RS28335 0.27 +2.1 529,168 - AZOBR_RS28335 0.27 +2.0
Or see this region's nucleotide sequence