Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS27725 and AZOBR_RS27730 are separated by 62 nucleotides AZOBR_RS27730 and AZOBR_RS27735 are separated by 62 nucleotides AZOBR_RS27735 and AZOBR_RS27740 are separated by 136 nucleotides
AZOBR_RS27725: AZOBR_RS27725 - hypothetical protein, at 386,481 to 386,765
_RS27725
AZOBR_RS27730: AZOBR_RS27730 - hypothetical protein, at 386,828 to 387,160
_RS27730
AZOBR_RS27735: AZOBR_RS27735 - hypothetical protein, at 387,223 to 387,675
_RS27735
AZOBR_RS27740: AZOBR_RS27740 - hypothetical protein, at 387,812 to 389,290
_RS27740
Position (kb) 387
388 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 386.901 kb on + strand, within AZOBR_RS27730 at 386.901 kb on + strand, within AZOBR_RS27730 at 387.509 kb on + strand, within AZOBR_RS27735 at 387.510 kb on - strand, within AZOBR_RS27735 at 387.510 kb on - strand, within AZOBR_RS27735 at 387.510 kb on - strand, within AZOBR_RS27735 at 387.510 kb on - strand, within AZOBR_RS27735 at 387.513 kb on - strand, within AZOBR_RS27735 at 388.053 kb on + strand, within AZOBR_RS27740 at 388.053 kb on + strand, within AZOBR_RS27740 at 388.053 kb on + strand, within AZOBR_RS27740 at 388.053 kb on + strand, within AZOBR_RS27740 at 388.053 kb on + strand, within AZOBR_RS27740 at 388.054 kb on - strand, within AZOBR_RS27740 at 388.630 kb on - strand, within AZOBR_RS27740
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 386,901 + AZOBR_RS27730 0.22 +0.5 386,901 + AZOBR_RS27730 0.22 -1.0 387,509 + AZOBR_RS27735 0.63 -1.3 387,510 - AZOBR_RS27735 0.63 -0.8 387,510 - AZOBR_RS27735 0.63 +0.1 387,510 - AZOBR_RS27735 0.63 -0.3 387,510 - AZOBR_RS27735 0.63 -4.4 387,513 - AZOBR_RS27735 0.64 +0.4 388,053 + AZOBR_RS27740 0.16 -2.3 388,053 + AZOBR_RS27740 0.16 +0.6 388,053 + AZOBR_RS27740 0.16 -0.2 388,053 + AZOBR_RS27740 0.16 -1.8 388,053 + AZOBR_RS27740 0.16 +0.2 388,054 - AZOBR_RS27740 0.16 +1.1 388,630 - AZOBR_RS27740 0.55 -2.9
Or see this region's nucleotide sequence