Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS27630 and AZOBR_RS27635 overlap by 1 nucleotides AZOBR_RS27635 and AZOBR_RS27640 are separated by 66 nucleotides
AZOBR_RS27630: AZOBR_RS27630 - CoA-transferase, at 361,198 to 362,391
_RS27630
AZOBR_RS27635: AZOBR_RS27635 - enoyl-CoA hydratase, at 362,391 to 363,212
_RS27635
AZOBR_RS27640: AZOBR_RS27640 - ABC transporter substrate-binding protein, at 363,279 to 364,250
_RS27640
Position (kb) 362
363
364 Strain fitness (log2 ratio) -2
-1
0
1
2 at 361.894 kb on + strand, within AZOBR_RS27630 at 362.152 kb on + strand, within AZOBR_RS27630 at 362.153 kb on - strand, within AZOBR_RS27630 at 362.153 kb on - strand, within AZOBR_RS27630 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.210 kb on + strand at 363.211 kb on - strand at 363.246 kb on + strand at 363.246 kb on + strand at 363.246 kb on + strand at 363.247 kb on - strand at 363.391 kb on - strand, within AZOBR_RS27640 at 363.391 kb on - strand, within AZOBR_RS27640 at 363.618 kb on + strand, within AZOBR_RS27640 at 363.619 kb on - strand, within AZOBR_RS27640 at 363.747 kb on + strand, within AZOBR_RS27640 at 363.748 kb on - strand, within AZOBR_RS27640 at 363.748 kb on - strand, within AZOBR_RS27640 at 363.900 kb on + strand, within AZOBR_RS27640 at 363.901 kb on - strand, within AZOBR_RS27640 at 364.002 kb on + strand, within AZOBR_RS27640 at 364.003 kb on - strand, within AZOBR_RS27640
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 361,894 + AZOBR_RS27630 0.58 -0.7 362,152 + AZOBR_RS27630 0.80 +1.5 362,153 - AZOBR_RS27630 0.80 -1.1 362,153 - AZOBR_RS27630 0.80 +1.9 363,013 - AZOBR_RS27635 0.76 -0.1 363,013 - AZOBR_RS27635 0.76 -0.6 363,013 - AZOBR_RS27635 0.76 -0.4 363,210 + -0.3 363,211 - +1.3 363,246 + -0.8 363,246 + +0.4 363,246 + +0.5 363,247 - -2.1 363,391 - AZOBR_RS27640 0.12 +0.1 363,391 - AZOBR_RS27640 0.12 +0.5 363,618 + AZOBR_RS27640 0.35 +1.0 363,619 - AZOBR_RS27640 0.35 +0.2 363,747 + AZOBR_RS27640 0.48 +0.0 363,748 - AZOBR_RS27640 0.48 -1.2 363,748 - AZOBR_RS27640 0.48 +1.1 363,900 + AZOBR_RS27640 0.64 -0.1 363,901 - AZOBR_RS27640 0.64 -1.8 364,002 + AZOBR_RS27640 0.74 +0.7 364,003 - AZOBR_RS27640 0.74 +0.8
Or see this region's nucleotide sequence