Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16660 and AZOBR_RS16665 are separated by 58 nucleotides AZOBR_RS16665 and AZOBR_RS16670 overlap by 8 nucleotides AZOBR_RS16670 and AZOBR_RS14370 are separated by 289 nucleotides AZOBR_RS14370 and AZOBR_RS16675 are separated by 38 nucleotides
AZOBR_RS16660: AZOBR_RS16660 - ABC transporter permease, at 494,647 to 495,849
_RS16660
AZOBR_RS16665: AZOBR_RS16665 - histidinol dehydrogenase, at 495,908 to 496,630
_RS16665
AZOBR_RS16670: AZOBR_RS16670 - amino acid ABC transporter ATP-binding protein, at 496,623 to 497,399
_RS16670
AZOBR_RS14370: AZOBR_RS14370 - cobalamin, at 497,689 to 497,905
_RS14370
AZOBR_RS16675: AZOBR_RS16675 - CobW, at 497,944 to 499,011
_RS16675
Position (kb) 496
497
498 Strain fitness (log2 ratio) -2
-1
0
1 at 495.731 kb on + strand at 495.731 kb on + strand at 495.731 kb on + strand at 495.732 kb on - strand at 495.732 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.922 kb on - strand at 495.999 kb on + strand, within AZOBR_RS16665 at 495.999 kb on + strand, within AZOBR_RS16665 at 496.260 kb on + strand, within AZOBR_RS16665 at 496.261 kb on - strand, within AZOBR_RS16665 at 497.467 kb on + strand at 497.740 kb on + strand, within AZOBR_RS14370 at 497.740 kb on + strand, within AZOBR_RS14370 at 497.741 kb on - strand, within AZOBR_RS14370 at 497.797 kb on + strand, within AZOBR_RS14370 at 497.798 kb on - strand, within AZOBR_RS14370
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 495,731 + -0.1 495,731 + -1.7 495,731 + +0.3 495,732 - -0.8 495,732 - -1.6 495,922 - +0.0 495,922 - -0.5 495,922 - +1.3 495,922 - +0.1 495,922 - -0.8 495,922 - +0.4 495,922 - +0.0 495,999 + AZOBR_RS16665 0.13 +0.5 495,999 + AZOBR_RS16665 0.13 -0.5 496,260 + AZOBR_RS16665 0.49 +0.6 496,261 - AZOBR_RS16665 0.49 -1.5 497,467 + -2.4 497,740 + AZOBR_RS14370 0.24 -2.2 497,740 + AZOBR_RS14370 0.24 -2.4 497,741 - AZOBR_RS14370 0.24 -0.1 497,797 + AZOBR_RS14370 0.50 -0.1 497,798 - AZOBR_RS14370 0.50 -0.9
Or see this region's nucleotide sequence