Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16315 and AZOBR_RS16320 are separated by 143 nucleotides AZOBR_RS16320 and AZOBR_RS16325 are separated by 121 nucleotides
AZOBR_RS16315: AZOBR_RS16315 - hypothetical protein, at 409,675 to 410,376
_RS16315
AZOBR_RS16320: AZOBR_RS16320 - phospholipid N-methyltransferase, at 410,520 to 411,134
_RS16320
AZOBR_RS16325: AZOBR_RS16325 - short-chain dehydrogenase, at 411,256 to 412,017
_RS16325
Position (kb) 409
410
411 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 409.541 kb on + strand at 409.542 kb on - strand at 409.542 kb on - strand at 409.646 kb on + strand at 409.646 kb on + strand at 409.647 kb on - strand at 409.674 kb on + strand at 409.675 kb on - strand at 410.356 kb on + strand at 410.374 kb on + strand at 410.374 kb on + strand at 410.374 kb on + strand at 410.374 kb on + strand at 410.375 kb on - strand at 410.375 kb on - strand at 410.421 kb on + strand at 410.421 kb on + strand at 410.422 kb on - strand at 410.422 kb on - strand at 410.422 kb on - strand at 410.422 kb on - strand at 410.442 kb on - strand at 410.474 kb on + strand at 410.633 kb on + strand, within AZOBR_RS16320 at 410.634 kb on - strand, within AZOBR_RS16320 at 411.009 kb on + strand, within AZOBR_RS16320 at 411.009 kb on + strand, within AZOBR_RS16320 at 411.015 kb on + strand, within AZOBR_RS16320 at 411.245 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 409,541 + -0.1 409,542 - -0.6 409,542 - +1.5 409,646 + +0.4 409,646 + +0.9 409,647 - +0.1 409,674 + +0.2 409,675 - +0.6 410,356 + -0.2 410,374 + -0.2 410,374 + +0.5 410,374 + -0.9 410,374 + +0.2 410,375 - +0.7 410,375 - -0.7 410,421 + +2.3 410,421 + +1.2 410,422 - +0.7 410,422 - -3.4 410,422 - +2.6 410,422 - -3.8 410,442 - +0.2 410,474 + +1.5 410,633 + AZOBR_RS16320 0.18 -4.3 410,634 - AZOBR_RS16320 0.19 +0.3 411,009 + AZOBR_RS16320 0.80 +1.5 411,009 + AZOBR_RS16320 0.80 +0.2 411,015 + AZOBR_RS16320 0.80 -1.4 411,245 - -0.3
Or see this region's nucleotide sequence