Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS15555 and AZOBR_RS15560 are separated by 26 nucleotides AZOBR_RS15560 and AZOBR_RS15565 are separated by 2 nucleotides AZOBR_RS15565 and AZOBR_RS15570 overlap by 4 nucleotides
AZOBR_RS15555: AZOBR_RS15555 - transcriptional regulator, at 244,787 to 245,683
_RS15555
AZOBR_RS15560: AZOBR_RS15560 - isopropylmalate isomerase, at 245,710 to 246,306
_RS15560
AZOBR_RS15565: AZOBR_RS15565 - isopropylmalate isomerase, at 246,309 to 247,715
_RS15565
AZOBR_RS15570: AZOBR_RS15570 - 3-ketosteroid dehydrogenase, at 247,712 to 249,133
_RS15570
Position (kb) 246
247
248 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 245.324 kb on + strand, within AZOBR_RS15555 at 245.324 kb on + strand, within AZOBR_RS15555 at 245.325 kb on - strand, within AZOBR_RS15555 at 245.325 kb on - strand, within AZOBR_RS15555 at 245.681 kb on + strand at 245.682 kb on - strand at 246.065 kb on + strand, within AZOBR_RS15560 at 246.066 kb on - strand, within AZOBR_RS15560 at 246.310 kb on + strand at 246.736 kb on + strand, within AZOBR_RS15565 at 246.736 kb on + strand, within AZOBR_RS15565 at 246.737 kb on - strand, within AZOBR_RS15565 at 246.737 kb on - strand, within AZOBR_RS15565 at 246.737 kb on - strand, within AZOBR_RS15565 at 246.960 kb on - strand, within AZOBR_RS15565 at 246.968 kb on - strand, within AZOBR_RS15565 at 246.968 kb on - strand, within AZOBR_RS15565 at 246.968 kb on - strand, within AZOBR_RS15565 at 247.519 kb on + strand, within AZOBR_RS15565 at 247.520 kb on - strand, within AZOBR_RS15565 at 247.520 kb on - strand, within AZOBR_RS15565 at 247.806 kb on + strand at 247.858 kb on - strand, within AZOBR_RS15570 at 247.977 kb on + strand, within AZOBR_RS15570 at 247.978 kb on - strand, within AZOBR_RS15570 at 248.262 kb on + strand, within AZOBR_RS15570 at 248.463 kb on + strand, within AZOBR_RS15570 at 248.521 kb on - strand, within AZOBR_RS15570 at 248.619 kb on + strand, within AZOBR_RS15570 at 248.620 kb on - strand, within AZOBR_RS15570
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 245,324 + AZOBR_RS15555 0.60 -0.7 245,324 + AZOBR_RS15555 0.60 -1.8 245,325 - AZOBR_RS15555 0.60 -1.5 245,325 - AZOBR_RS15555 0.60 -0.7 245,681 + +1.3 245,682 - -1.0 246,065 + AZOBR_RS15560 0.59 +0.2 246,066 - AZOBR_RS15560 0.60 +0.9 246,310 + -1.3 246,736 + AZOBR_RS15565 0.30 +0.4 246,736 + AZOBR_RS15565 0.30 +0.5 246,737 - AZOBR_RS15565 0.30 -2.7 246,737 - AZOBR_RS15565 0.30 +0.9 246,737 - AZOBR_RS15565 0.30 -0.5 246,960 - AZOBR_RS15565 0.46 -0.1 246,968 - AZOBR_RS15565 0.47 -3.2 246,968 - AZOBR_RS15565 0.47 -1.5 246,968 - AZOBR_RS15565 0.47 +0.3 247,519 + AZOBR_RS15565 0.86 +1.7 247,520 - AZOBR_RS15565 0.86 +0.8 247,520 - AZOBR_RS15565 0.86 +1.0 247,806 + +0.5 247,858 - AZOBR_RS15570 0.10 +0.3 247,977 + AZOBR_RS15570 0.19 -0.4 247,978 - AZOBR_RS15570 0.19 +0.4 248,262 + AZOBR_RS15570 0.39 +0.1 248,463 + AZOBR_RS15570 0.53 +0.7 248,521 - AZOBR_RS15570 0.57 -1.0 248,619 + AZOBR_RS15570 0.64 +2.4 248,620 - AZOBR_RS15570 0.64 +0.8
Or see this region's nucleotide sequence