Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS10330 and AZOBR_RS10335 overlap by 1 nucleotides AZOBR_RS10335 and AZOBR_RS10340 are separated by 26 nucleotides AZOBR_RS10340 and AZOBR_RS10345 overlap by 4 nucleotides
AZOBR_RS10330: AZOBR_RS10330 - glycosyl transferase, at 2,170,325 to 2,171,476
_RS10330
AZOBR_RS10335: AZOBR_RS10335 - acyltransferase, at 2,171,476 to 2,172,294
_RS10335
AZOBR_RS10340: AZOBR_RS10340 - phytoene desaturase, at 2,172,321 to 2,173,862
_RS10340
AZOBR_RS10345: AZOBR_RS10345 - phytoene desaturase, at 2,173,859 to 2,175,412
_RS10345
Position (kb)
2172
2173
2174 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2171.524 kb on - strand at 2171.786 kb on + strand, within AZOBR_RS10335 at 2171.787 kb on - strand, within AZOBR_RS10335 at 2172.050 kb on + strand, within AZOBR_RS10335 at 2172.336 kb on + strand at 2172.336 kb on + strand at 2172.454 kb on + strand at 2172.454 kb on + strand at 2172.454 kb on + strand at 2172.454 kb on + strand at 2172.455 kb on - strand at 2172.529 kb on + strand, within AZOBR_RS10340 at 2172.529 kb on + strand, within AZOBR_RS10340 at 2172.529 kb on + strand, within AZOBR_RS10340 at 2172.724 kb on + strand, within AZOBR_RS10340 at 2172.724 kb on + strand, within AZOBR_RS10340 at 2172.725 kb on - strand, within AZOBR_RS10340 at 2172.725 kb on - strand, within AZOBR_RS10340 at 2172.725 kb on - strand, within AZOBR_RS10340 at 2172.784 kb on + strand, within AZOBR_RS10340 at 2172.784 kb on + strand, within AZOBR_RS10340 at 2172.784 kb on + strand, within AZOBR_RS10340 at 2172.785 kb on - strand, within AZOBR_RS10340 at 2172.785 kb on - strand, within AZOBR_RS10340 at 2172.785 kb on - strand, within AZOBR_RS10340 at 2172.820 kb on + strand, within AZOBR_RS10340 at 2172.907 kb on + strand, within AZOBR_RS10340 at 2172.991 kb on + strand, within AZOBR_RS10340 at 2172.992 kb on - strand, within AZOBR_RS10340 at 2172.992 kb on - strand, within AZOBR_RS10340 at 2172.992 kb on - strand, within AZOBR_RS10340 at 2172.992 kb on - strand, within AZOBR_RS10340 at 2173.264 kb on + strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.265 kb on - strand, within AZOBR_RS10340 at 2173.492 kb on + strand, within AZOBR_RS10340 at 2174.031 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.298 kb on + strand, within AZOBR_RS10345 at 2174.299 kb on - strand at 2174.299 kb on - strand, within AZOBR_RS10345 at 2174.299 kb on - strand, within AZOBR_RS10345 at 2174.299 kb on - strand, within AZOBR_RS10345 at 2174.358 kb on + strand, within AZOBR_RS10345 at 2174.358 kb on + strand, within AZOBR_RS10345 at 2174.358 kb on + strand, within AZOBR_RS10345 at 2174.358 kb on + strand, within AZOBR_RS10345 at 2174.359 kb on - strand, within AZOBR_RS10345 at 2174.359 kb on - strand, within AZOBR_RS10345 at 2174.359 kb on - strand, within AZOBR_RS10345 at 2174.359 kb on - strand, within AZOBR_RS10345 at 2174.418 kb on + strand, within AZOBR_RS10345 at 2174.418 kb on + strand, within AZOBR_RS10345 at 2174.418 kb on + strand, within AZOBR_RS10345 at 2174.419 kb on - strand, within AZOBR_RS10345 at 2174.433 kb on + strand, within AZOBR_RS10345 at 2174.433 kb on + strand, within AZOBR_RS10345 at 2174.434 kb on - strand, within AZOBR_RS10345 at 2174.481 kb on + strand, within AZOBR_RS10345 at 2174.482 kb on - strand, within AZOBR_RS10345 at 2174.617 kb on - strand, within AZOBR_RS10345 at 2174.842 kb on - strand, within AZOBR_RS10345 at 2174.842 kb on - strand, within AZOBR_RS10345
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,171,524 - -0.4 2,171,786 + AZOBR_RS10335 0.38 -0.3 2,171,787 - AZOBR_RS10335 0.38 -0.0 2,172,050 + AZOBR_RS10335 0.70 -0.2 2,172,336 + +1.0 2,172,336 + +0.0 2,172,454 + +0.4 2,172,454 + -1.1 2,172,454 + +3.6 2,172,454 + -1.5 2,172,455 - +0.7 2,172,529 + AZOBR_RS10340 0.13 +1.8 2,172,529 + AZOBR_RS10340 0.13 -1.9 2,172,529 + AZOBR_RS10340 0.13 +1.4 2,172,724 + AZOBR_RS10340 0.26 +1.6 2,172,724 + AZOBR_RS10340 0.26 -2.5 2,172,725 - AZOBR_RS10340 0.26 -0.8 2,172,725 - AZOBR_RS10340 0.26 -3.5 2,172,725 - AZOBR_RS10340 0.26 -0.9 2,172,784 + AZOBR_RS10340 0.30 +0.8 2,172,784 + AZOBR_RS10340 0.30 -4.0 2,172,784 + AZOBR_RS10340 0.30 +0.1 2,172,785 - AZOBR_RS10340 0.30 -0.8 2,172,785 - AZOBR_RS10340 0.30 +0.9 2,172,785 - AZOBR_RS10340 0.30 -3.7 2,172,820 + AZOBR_RS10340 0.32 -1.1 2,172,907 + AZOBR_RS10340 0.38 -1.0 2,172,991 + AZOBR_RS10340 0.43 +0.2 2,172,992 - AZOBR_RS10340 0.44 +0.0 2,172,992 - AZOBR_RS10340 0.44 -0.6 2,172,992 - AZOBR_RS10340 0.44 -2.9 2,172,992 - AZOBR_RS10340 0.44 +0.1 2,173,264 + AZOBR_RS10340 0.61 +2.6 2,173,265 - AZOBR_RS10340 0.61 +0.1 2,173,265 - AZOBR_RS10340 0.61 -0.9 2,173,265 - AZOBR_RS10340 0.61 -2.1 2,173,265 - AZOBR_RS10340 0.61 +0.9 2,173,265 - AZOBR_RS10340 0.61 +0.7 2,173,265 - AZOBR_RS10340 0.61 +0.5 2,173,492 + AZOBR_RS10340 0.76 -0.2 2,174,031 + AZOBR_RS10345 0.11 -0.1 2,174,298 + AZOBR_RS10345 0.28 +1.2 2,174,298 + AZOBR_RS10345 0.28 +1.6 2,174,298 + AZOBR_RS10345 0.28 +0.1 2,174,298 + AZOBR_RS10345 0.28 +0.2 2,174,298 + AZOBR_RS10345 0.28 +0.6 2,174,298 + AZOBR_RS10345 0.28 +0.4 2,174,299 - +0.4 2,174,299 - AZOBR_RS10345 0.28 +0.0 2,174,299 - AZOBR_RS10345 0.28 -0.1 2,174,299 - AZOBR_RS10345 0.28 -1.3 2,174,358 + AZOBR_RS10345 0.32 +0.3 2,174,358 + AZOBR_RS10345 0.32 +0.5 2,174,358 + AZOBR_RS10345 0.32 +0.7 2,174,358 + AZOBR_RS10345 0.32 -1.0 2,174,359 - AZOBR_RS10345 0.32 -1.2 2,174,359 - AZOBR_RS10345 0.32 +0.4 2,174,359 - AZOBR_RS10345 0.32 +0.5 2,174,359 - AZOBR_RS10345 0.32 -0.8 2,174,418 + AZOBR_RS10345 0.36 -1.2 2,174,418 + AZOBR_RS10345 0.36 -0.3 2,174,418 + AZOBR_RS10345 0.36 -2.7 2,174,419 - AZOBR_RS10345 0.36 +0.3 2,174,433 + AZOBR_RS10345 0.37 -0.2 2,174,433 + AZOBR_RS10345 0.37 -1.7 2,174,434 - AZOBR_RS10345 0.37 +0.4 2,174,481 + AZOBR_RS10345 0.40 -3.8 2,174,482 - AZOBR_RS10345 0.40 +1.6 2,174,617 - AZOBR_RS10345 0.49 +0.1 2,174,842 - AZOBR_RS10345 0.63 -1.0 2,174,842 - AZOBR_RS10345 0.63 -1.1
Or see this region's nucleotide sequence