Strain Fitness in Azospirillum brasilense Sp245 around AZOBR_RS05070

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAZOBR_RS05060 and AZOBR_RS05065 are separated by 224 nucleotidesAZOBR_RS05065 and AZOBR_RS05070 are separated by 144 nucleotidesAZOBR_RS05070 and AZOBR_RS05075 are separated by 147 nucleotides AZOBR_RS05060: AZOBR_RS05060 - zinc protease, at 1,072,901 to 1,074,223 _RS05060 AZOBR_RS05065: AZOBR_RS05065 - hypothetical protein, at 1,074,448 to 1,074,993 _RS05065 AZOBR_RS05070: AZOBR_RS05070 - peptidase A8, at 1,075,138 to 1,075,641 _RS05070 AZOBR_RS05075: AZOBR_RS05075 - isoleucyl-tRNA synthetase, at 1,075,789 to 1,078,644 _RS05075 Position (kb) 1075 1076Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1074.375 kb on + strandat 1074.376 kb on - strandat 1074.376 kb on - strandat 1074.449 kb on + strandat 1074.450 kb on - strandat 1074.450 kb on - strandat 1074.579 kb on - strand, within AZOBR_RS05065at 1074.579 kb on - strand, within AZOBR_RS05065at 1075.077 kb on + strandat 1075.113 kb on + strandat 1075.115 kb on + strandat 1075.115 kb on + strandat 1075.116 kb on - strandat 1075.137 kb on - strandat 1075.137 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,074,375 + -3.9
1,074,376 - +0.1
1,074,376 - -0.4
1,074,449 + -1.0
1,074,450 - -2.1
1,074,450 - -1.7
1,074,579 - AZOBR_RS05065 0.24 +0.2
1,074,579 - AZOBR_RS05065 0.24 -1.0
1,075,077 + -1.5
1,075,113 + +1.0
1,075,115 + -1.3
1,075,115 + +2.7
1,075,116 - -0.5
1,075,137 - -2.7
1,075,137 - +0.0

Or see this region's nucleotide sequence