Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04265 and AZOBR_RS04270 are separated by 16 nucleotides AZOBR_RS04270 and AZOBR_RS04275 are separated by 53 nucleotides AZOBR_RS04275 and AZOBR_RS04280 are separated by 156 nucleotides AZOBR_RS04280 and AZOBR_RS04285 are separated by 154 nucleotides
AZOBR_RS04265: AZOBR_RS04265 - 2-isopropylmalate synthase, at 899,574 to 901,178
_RS04265
AZOBR_RS04270: AZOBR_RS04270 - RNA methyltransferase, at 901,195 to 901,965
_RS04270
AZOBR_RS04275: AZOBR_RS04275 - response regulator, at 902,019 to 902,732
_RS04275
AZOBR_RS04280: AZOBR_RS04280 - hypothetical protein, at 902,889 to 903,101
_RS04280
AZOBR_RS04285: AZOBR_RS04285 - nitrogen fixation protein CowN, at 903,256 to 903,552
_RS04285
Position (kb) 902
903 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 901.042 kb on + strand at 901.043 kb on - strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.135 kb on - strand at 901.151 kb on + strand at 901.417 kb on + strand, within AZOBR_RS04270 at 901.418 kb on - strand, within AZOBR_RS04270 at 901.694 kb on - strand, within AZOBR_RS04270 at 901.963 kb on + strand at 902.008 kb on + strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.025 kb on + strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.040 kb on - strand at 902.466 kb on + strand, within AZOBR_RS04275 at 902.467 kb on - strand, within AZOBR_RS04275 at 902.467 kb on - strand, within AZOBR_RS04275 at 902.487 kb on + strand, within AZOBR_RS04275 at 902.488 kb on - strand, within AZOBR_RS04275 at 902.826 kb on + strand at 902.826 kb on + strand at 902.826 kb on + strand at 902.826 kb on + strand at 902.826 kb on + strand at 902.827 kb on - strand at 902.883 kb on - strand at 902.883 kb on - strand at 902.916 kb on + strand, within AZOBR_RS04280 at 902.916 kb on + strand, within AZOBR_RS04280 at 902.916 kb on + strand, within AZOBR_RS04280 at 902.917 kb on - strand, within AZOBR_RS04280 at 902.917 kb on - strand, within AZOBR_RS04280 at 902.917 kb on - strand, within AZOBR_RS04280 at 902.941 kb on - strand, within AZOBR_RS04280 at 902.944 kb on - strand, within AZOBR_RS04280 at 903.120 kb on - strand at 903.120 kb on - strand at 903.343 kb on + strand, within AZOBR_RS04285 at 903.344 kb on - strand, within AZOBR_RS04285 at 903.344 kb on - strand, within AZOBR_RS04285 at 903.469 kb on + strand, within AZOBR_RS04285 at 903.469 kb on + strand, within AZOBR_RS04285 at 903.470 kb on - strand, within AZOBR_RS04285 at 903.472 kb on + strand, within AZOBR_RS04285 at 903.472 kb on + strand, within AZOBR_RS04285 at 903.472 kb on + strand, within AZOBR_RS04285 at 903.472 kb on + strand, within AZOBR_RS04285 at 903.472 kb on + strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.473 kb on - strand, within AZOBR_RS04285 at 903.496 kb on + strand, within AZOBR_RS04285 at 903.496 kb on + strand, within AZOBR_RS04285 at 903.496 kb on + strand, within AZOBR_RS04285 at 903.497 kb on - strand, within AZOBR_RS04285
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 901,042 + -0.2 901,043 - -1.0 901,134 + +1.2 901,134 + -1.0 901,134 + -1.7 901,135 - +1.2 901,151 + -1.3 901,417 + AZOBR_RS04270 0.29 +0.4 901,418 - AZOBR_RS04270 0.29 +0.7 901,694 - AZOBR_RS04270 0.65 -1.4 901,963 + +0.6 902,008 + +0.2 902,009 - -2.1 902,009 - +0.3 902,009 - +1.3 902,009 - -2.3 902,010 + +1.2 902,010 + +0.9 902,010 + -0.1 902,011 - +0.2 902,011 - +0.1 902,011 - -2.5 902,025 + -3.3 902,026 - +1.0 902,026 - -3.1 902,026 - -2.8 902,026 - -0.2 902,040 - -1.3 902,466 + AZOBR_RS04275 0.63 +1.4 902,467 - AZOBR_RS04275 0.63 -1.3 902,467 - AZOBR_RS04275 0.63 -0.6 902,487 + AZOBR_RS04275 0.66 -2.6 902,488 - AZOBR_RS04275 0.66 -1.3 902,826 + +1.0 902,826 + +0.6 902,826 + -2.4 902,826 + -1.8 902,826 + +0.9 902,827 - -0.7 902,883 - +1.1 902,883 - -0.1 902,916 + AZOBR_RS04280 0.13 +1.8 902,916 + AZOBR_RS04280 0.13 -1.7 902,916 + AZOBR_RS04280 0.13 +0.9 902,917 - AZOBR_RS04280 0.13 -0.1 902,917 - AZOBR_RS04280 0.13 -0.8 902,917 - AZOBR_RS04280 0.13 -0.7 902,941 - AZOBR_RS04280 0.24 -0.7 902,944 - AZOBR_RS04280 0.26 -2.4 903,120 - +3.1 903,120 - -0.7 903,343 + AZOBR_RS04285 0.29 +0.3 903,344 - AZOBR_RS04285 0.30 -3.4 903,344 - AZOBR_RS04285 0.30 +1.3 903,469 + AZOBR_RS04285 0.72 -0.8 903,469 + AZOBR_RS04285 0.72 -0.4 903,470 - AZOBR_RS04285 0.72 +0.9 903,472 + AZOBR_RS04285 0.73 +1.2 903,472 + AZOBR_RS04285 0.73 -0.4 903,472 + AZOBR_RS04285 0.73 -0.4 903,472 + AZOBR_RS04285 0.73 -3.3 903,472 + AZOBR_RS04285 0.73 +0.1 903,473 - AZOBR_RS04285 0.73 +0.2 903,473 - AZOBR_RS04285 0.73 +0.5 903,473 - AZOBR_RS04285 0.73 -3.0 903,473 - AZOBR_RS04285 0.73 +1.6 903,473 - AZOBR_RS04285 0.73 -2.6 903,473 - AZOBR_RS04285 0.73 +0.7 903,473 - AZOBR_RS04285 0.73 +0.2 903,473 - AZOBR_RS04285 0.73 -0.9 903,473 - AZOBR_RS04285 0.73 +1.3 903,473 - AZOBR_RS04285 0.73 -3.4 903,496 + AZOBR_RS04285 0.81 +0.9 903,496 + AZOBR_RS04285 0.81 +1.7 903,496 + AZOBR_RS04285 0.81 +0.7 903,497 - AZOBR_RS04285 0.81 +0.5
Or see this region's nucleotide sequence