Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03695 and AZOBR_RS03700 are separated by 133 nucleotides AZOBR_RS03700 and AZOBR_RS03705 are separated by 8 nucleotides AZOBR_RS03705 and AZOBR_RS03710 are separated by 158 nucleotides
AZOBR_RS03695: AZOBR_RS03695 - NAD synthetase, at 779,002 to 779,910
_RS03695
AZOBR_RS03700: AZOBR_RS03700 - hypothetical protein, at 780,044 to 780,508
_RS03700
AZOBR_RS03705: AZOBR_RS03705 - glutathione S-transferase, at 780,517 to 781,137
_RS03705
AZOBR_RS03710: AZOBR_RS03710 - coproporphyrinogen III oxidase, at 781,296 to 782,639
_RS03710
Position (kb)
780
781
782 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 779.575 kb on + strand, within AZOBR_RS03695 at 779.576 kb on - strand, within AZOBR_RS03695 at 779.576 kb on - strand, within AZOBR_RS03695 at 780.102 kb on + strand, within AZOBR_RS03700 at 780.103 kb on - strand, within AZOBR_RS03700 at 780.103 kb on - strand, within AZOBR_RS03700 at 780.103 kb on - strand, within AZOBR_RS03700 at 780.213 kb on + strand, within AZOBR_RS03700 at 780.342 kb on + strand, within AZOBR_RS03700 at 780.342 kb on + strand, within AZOBR_RS03700 at 780.342 kb on + strand, within AZOBR_RS03700 at 780.343 kb on - strand, within AZOBR_RS03700 at 780.381 kb on + strand, within AZOBR_RS03700 at 780.381 kb on + strand, within AZOBR_RS03700 at 780.381 kb on + strand, within AZOBR_RS03700 at 780.381 kb on + strand, within AZOBR_RS03700 at 780.382 kb on - strand, within AZOBR_RS03700 at 780.382 kb on - strand, within AZOBR_RS03700 at 780.382 kb on - strand, within AZOBR_RS03700 at 780.382 kb on - strand, within AZOBR_RS03700 at 780.686 kb on + strand, within AZOBR_RS03705 at 780.750 kb on - strand, within AZOBR_RS03705 at 780.750 kb on - strand, within AZOBR_RS03705 at 780.761 kb on + strand, within AZOBR_RS03705 at 781.011 kb on - strand at 781.011 kb on - strand, within AZOBR_RS03705 at 781.061 kb on + strand, within AZOBR_RS03705 at 781.061 kb on + strand at 781.062 kb on - strand, within AZOBR_RS03705 at 781.336 kb on + strand at 781.336 kb on + strand at 781.336 kb on + strand at 781.337 kb on - strand at 781.337 kb on - strand at 781.337 kb on - strand at 781.337 kb on - strand at 781.654 kb on + strand, within AZOBR_RS03710 at 781.654 kb on + strand, within AZOBR_RS03710 at 781.654 kb on + strand, within AZOBR_RS03710 at 781.655 kb on - strand, within AZOBR_RS03710 at 781.655 kb on - strand, within AZOBR_RS03710 at 781.655 kb on - strand, within AZOBR_RS03710 at 781.655 kb on - strand, within AZOBR_RS03710 at 781.723 kb on + strand, within AZOBR_RS03710 at 781.724 kb on - strand, within AZOBR_RS03710 at 782.026 kb on + strand, within AZOBR_RS03710 at 782.026 kb on + strand, within AZOBR_RS03710 at 782.027 kb on - strand, within AZOBR_RS03710 at 782.027 kb on - strand, within AZOBR_RS03710 at 782.027 kb on - strand, within AZOBR_RS03710 at 782.027 kb on - strand, within AZOBR_RS03710 at 782.027 kb on - strand, within AZOBR_RS03710 at 782.045 kb on + strand, within AZOBR_RS03710 at 782.046 kb on - strand, within AZOBR_RS03710 at 782.046 kb on - strand, within AZOBR_RS03710 at 782.046 kb on - strand, within AZOBR_RS03710
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 779,575 + AZOBR_RS03695 0.63 +2.1 779,576 - AZOBR_RS03695 0.63 -1.2 779,576 - AZOBR_RS03695 0.63 +1.1 780,102 + AZOBR_RS03700 0.12 -2.9 780,103 - AZOBR_RS03700 0.13 -3.5 780,103 - AZOBR_RS03700 0.13 -0.1 780,103 - AZOBR_RS03700 0.13 -0.4 780,213 + AZOBR_RS03700 0.36 -1.1 780,342 + AZOBR_RS03700 0.64 +1.8 780,342 + AZOBR_RS03700 0.64 +0.3 780,342 + AZOBR_RS03700 0.64 -0.5 780,343 - AZOBR_RS03700 0.64 -1.4 780,381 + AZOBR_RS03700 0.72 -3.4 780,381 + AZOBR_RS03700 0.72 +0.4 780,381 + AZOBR_RS03700 0.72 +1.2 780,381 + AZOBR_RS03700 0.72 +1.7 780,382 - AZOBR_RS03700 0.73 -1.2 780,382 - AZOBR_RS03700 0.73 +0.5 780,382 - AZOBR_RS03700 0.73 -0.3 780,382 - AZOBR_RS03700 0.73 -3.3 780,686 + AZOBR_RS03705 0.27 -1.0 780,750 - AZOBR_RS03705 0.38 -0.3 780,750 - AZOBR_RS03705 0.38 -2.3 780,761 + AZOBR_RS03705 0.39 -1.3 781,011 - +2.2 781,011 - AZOBR_RS03705 0.80 -2.7 781,061 + AZOBR_RS03705 0.88 -3.6 781,061 + +2.2 781,062 - AZOBR_RS03705 0.88 -0.7 781,336 + -0.2 781,336 + +1.1 781,336 + -0.0 781,337 - +0.4 781,337 - +2.6 781,337 - -1.0 781,337 - -1.2 781,654 + AZOBR_RS03710 0.27 +0.6 781,654 + AZOBR_RS03710 0.27 -3.3 781,654 + AZOBR_RS03710 0.27 +2.6 781,655 - AZOBR_RS03710 0.27 -0.2 781,655 - AZOBR_RS03710 0.27 -0.7 781,655 - AZOBR_RS03710 0.27 +1.7 781,655 - AZOBR_RS03710 0.27 +0.2 781,723 + AZOBR_RS03710 0.32 +0.7 781,724 - AZOBR_RS03710 0.32 -1.8 782,026 + AZOBR_RS03710 0.54 +0.2 782,026 + AZOBR_RS03710 0.54 -1.0 782,027 - AZOBR_RS03710 0.54 +0.9 782,027 - AZOBR_RS03710 0.54 +1.7 782,027 - AZOBR_RS03710 0.54 -1.7 782,027 - AZOBR_RS03710 0.54 -0.3 782,027 - AZOBR_RS03710 0.54 +0.2 782,045 + AZOBR_RS03710 0.56 -0.4 782,046 - AZOBR_RS03710 0.56 -1.2 782,046 - AZOBR_RS03710 0.56 +3.2 782,046 - AZOBR_RS03710 0.56 -1.3
Or see this region's nucleotide sequence