Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03060 and AZOBR_RS03065 overlap by 23 nucleotides AZOBR_RS03065 and AZOBR_RS03070 are separated by 89 nucleotides AZOBR_RS03070 and AZOBR_RS03075 are separated by 169 nucleotides AZOBR_RS03075 and AZOBR_RS03080 are separated by 141 nucleotides
AZOBR_RS03060: AZOBR_RS03060 - molybdopterin biosynthesis protein MoeA, at 653,302 to 655,272
_RS03060
AZOBR_RS03065: AZOBR_RS03065 - pirin, at 655,250 to 656,152
_RS03065
AZOBR_RS03070: AZOBR_RS03070 - hypothetical protein, at 656,242 to 656,520
_RS03070
AZOBR_RS03075: AZOBR_RS03075 - tRNA-Ala, at 656,690 to 656,765
_RS03075
AZOBR_RS03080: AZOBR_RS03080 - hypothetical protein, at 656,907 to 657,305
_RS03080
Position (kb) 655
656
657 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 654.421 kb on + strand, within AZOBR_RS03060 at 654.422 kb on - strand, within AZOBR_RS03060 at 654.422 kb on - strand, within AZOBR_RS03060 at 654.422 kb on - strand, within AZOBR_RS03060 at 655.145 kb on - strand at 655.525 kb on - strand, within AZOBR_RS03065 at 655.525 kb on - strand, within AZOBR_RS03065 at 655.628 kb on + strand, within AZOBR_RS03065 at 655.628 kb on + strand, within AZOBR_RS03065 at 655.628 kb on + strand, within AZOBR_RS03065 at 655.628 kb on + strand, within AZOBR_RS03065 at 655.629 kb on - strand, within AZOBR_RS03065 at 655.636 kb on - strand, within AZOBR_RS03065 at 655.636 kb on - strand, within AZOBR_RS03065 at 655.920 kb on + strand, within AZOBR_RS03065 at 655.920 kb on + strand, within AZOBR_RS03065 at 656.725 kb on - strand, within AZOBR_RS03075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 654,421 + AZOBR_RS03060 0.57 +1.0 654,422 - AZOBR_RS03060 0.57 +0.5 654,422 - AZOBR_RS03060 0.57 +1.2 654,422 - AZOBR_RS03060 0.57 +0.7 655,145 - +0.8 655,525 - AZOBR_RS03065 0.30 -0.5 655,525 - AZOBR_RS03065 0.30 -1.5 655,628 + AZOBR_RS03065 0.42 +0.7 655,628 + AZOBR_RS03065 0.42 +0.9 655,628 + AZOBR_RS03065 0.42 -0.2 655,628 + AZOBR_RS03065 0.42 -1.8 655,629 - AZOBR_RS03065 0.42 -1.8 655,636 - AZOBR_RS03065 0.43 -2.8 655,636 - AZOBR_RS03065 0.43 -1.1 655,920 + AZOBR_RS03065 0.74 -0.9 655,920 + AZOBR_RS03065 0.74 -1.0 656,725 - AZOBR_RS03075 0.46 +0.4
Or see this region's nucleotide sequence