Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00305 and AZOBR_RS00310 are separated by 105 nucleotides AZOBR_RS00310 and AZOBR_RS00315 are separated by 75 nucleotides
AZOBR_RS00305: AZOBR_RS00305 - porphobilinogen deaminase, at 54,555 to 55,481
_RS00305
AZOBR_RS00310: AZOBR_RS00310 - ribosomal RNA small subunit methyltransferase C, at 55,587 to 56,423
_RS00310
AZOBR_RS00315: AZOBR_RS00315 - tRNA threonylcarbamoyladenosine modification protein TsaD, at 56,499 to 57,572
_RS00315
Position (kb) 55
56
57 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 55.538 kb on + strand at 55.742 kb on - strand, within AZOBR_RS00310 at 55.846 kb on + strand, within AZOBR_RS00310 at 55.846 kb on + strand, within AZOBR_RS00310 at 55.846 kb on + strand, within AZOBR_RS00310 at 55.846 kb on + strand, within AZOBR_RS00310 at 55.846 kb on + strand, within AZOBR_RS00310 at 55.847 kb on - strand, within AZOBR_RS00310 at 55.847 kb on - strand, within AZOBR_RS00310 at 55.847 kb on - strand, within AZOBR_RS00310 at 55.847 kb on - strand, within AZOBR_RS00310 at 56.284 kb on + strand, within AZOBR_RS00310 at 56.284 kb on + strand, within AZOBR_RS00310 at 56.285 kb on - strand, within AZOBR_RS00310 at 56.285 kb on - strand, within AZOBR_RS00310 at 56.285 kb on - strand, within AZOBR_RS00310 at 56.380 kb on + strand at 56.381 kb on - strand at 56.383 kb on + strand at 56.384 kb on - strand at 56.384 kb on - strand at 56.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 55,538 + -1.8 55,742 - AZOBR_RS00310 0.19 +0.8 55,846 + AZOBR_RS00310 0.31 +0.3 55,846 + AZOBR_RS00310 0.31 -2.4 55,846 + AZOBR_RS00310 0.31 +3.7 55,846 + AZOBR_RS00310 0.31 -0.5 55,846 + AZOBR_RS00310 0.31 +1.2 55,847 - AZOBR_RS00310 0.31 +0.2 55,847 - AZOBR_RS00310 0.31 +1.2 55,847 - AZOBR_RS00310 0.31 +0.8 55,847 - AZOBR_RS00310 0.31 +1.0 56,284 + AZOBR_RS00310 0.83 -1.0 56,284 + AZOBR_RS00310 0.83 -1.0 56,285 - AZOBR_RS00310 0.83 +1.5 56,285 - AZOBR_RS00310 0.83 -0.7 56,285 - AZOBR_RS00310 0.83 +0.9 56,380 + -1.9 56,381 - -2.6 56,383 + +0.1 56,384 - +0.7 56,384 - +0.1 56,462 + +0.0
Or see this region's nucleotide sequence