Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00050 and AZOBR_RS00055 are separated by 86 nucleotides AZOBR_RS00055 and AZOBR_RS00060 are separated by 18 nucleotides
AZOBR_RS00050: AZOBR_RS00050 - transposase, at 79 to 471
_RS00050
AZOBR_RS00055: AZOBR_RS00055 - transposase, at 558 to 836
_RS00055
AZOBR_RS00060: AZOBR_RS00060 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein, at 855 to 1,928
_RS00060
Position (kb) 0
1 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 0.309 kb on + strand, within AZOBR_RS00050 at 0.309 kb on + strand, within AZOBR_RS00050 at 0.594 kb on + strand, within AZOBR_RS00055 at 0.594 kb on + strand, within AZOBR_RS00055 at 0.595 kb on - strand, within AZOBR_RS00055 at 0.693 kb on + strand, within AZOBR_RS00055 at 0.693 kb on + strand, within AZOBR_RS00055 at 0.694 kb on - strand, within AZOBR_RS00055 at 0.699 kb on + strand, within AZOBR_RS00055 at 0.700 kb on - strand, within AZOBR_RS00055 at 0.769 kb on - strand, within AZOBR_RS00055 at 0.825 kb on + strand at 0.826 kb on - strand at 0.833 kb on - strand at 0.833 kb on - strand at 0.833 kb on - strand at 0.833 kb on - strand at 1.000 kb on + strand, within AZOBR_RS00060 at 1.001 kb on - strand, within AZOBR_RS00060 at 1.001 kb on - strand, within AZOBR_RS00060 at 1.010 kb on + strand, within AZOBR_RS00060 at 1.264 kb on + strand, within AZOBR_RS00060 at 1.265 kb on - strand, within AZOBR_RS00060 at 1.363 kb on + strand, within AZOBR_RS00060 at 1.363 kb on + strand, within AZOBR_RS00060 at 1.364 kb on - strand, within AZOBR_RS00060 at 1.364 kb on - strand, within AZOBR_RS00060
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 309 + AZOBR_RS00050 0.59 -3.6 309 + AZOBR_RS00050 0.59 -2.4 594 + AZOBR_RS00055 0.13 +1.2 594 + AZOBR_RS00055 0.13 -0.5 595 - AZOBR_RS00055 0.13 -0.2 693 + AZOBR_RS00055 0.48 +0.8 693 + AZOBR_RS00055 0.48 +2.6 694 - AZOBR_RS00055 0.49 -3.0 699 + AZOBR_RS00055 0.51 +1.7 700 - AZOBR_RS00055 0.51 -1.8 769 - AZOBR_RS00055 0.76 +0.4 825 + +0.1 826 - -0.3 833 - +0.2 833 - +1.3 833 - +2.5 833 - +0.2 1,000 + AZOBR_RS00060 0.14 -0.4 1,001 - AZOBR_RS00060 0.14 +1.9 1,001 - AZOBR_RS00060 0.14 +1.5 1,010 + AZOBR_RS00060 0.14 -1.5 1,264 + AZOBR_RS00060 0.38 +0.8 1,265 - AZOBR_RS00060 0.38 +1.2 1,363 + AZOBR_RS00060 0.47 +0.8 1,363 + AZOBR_RS00060 0.47 -2.5 1,364 - AZOBR_RS00060 0.47 -0.6 1,364 - AZOBR_RS00060 0.47 +0.5
Or see this region's nucleotide sequence