Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23395 and AO353_23400 are separated by 143 nucleotides AO353_23400 and AO353_23405 are separated by 194 nucleotides AO353_23405 and AO353_23410 are separated by 69 nucleotides AO353_23410 and AO353_23415 are separated by 276 nucleotides
AO353_23395: AO353_23395 - hypothetical protein, at 5,127,865 to 5,128,494
_23395
AO353_23400: AO353_23400 - hypothetical protein, at 5,128,638 to 5,128,967
_23400
AO353_23405: AO353_23405 - hypothetical protein, at 5,129,162 to 5,129,362
_23405
AO353_23410: AO353_23410 - hypothetical protein, at 5,129,432 to 5,129,716
_23410
AO353_23415: AO353_23415 - lysine transporter LysE, at 5,129,993 to 5,130,580
_23415
Position (kb) 5129
5130 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5128.163 kb on + strand, within AO353_23395 at 5128.164 kb on - strand at 5128.229 kb on + strand, within AO353_23395 at 5128.230 kb on - strand, within AO353_23395 at 5128.271 kb on - strand, within AO353_23395 at 5128.331 kb on + strand, within AO353_23395 at 5128.331 kb on + strand, within AO353_23395 at 5128.332 kb on - strand, within AO353_23395 at 5128.350 kb on + strand, within AO353_23395 at 5128.350 kb on + strand, within AO353_23395 at 5128.351 kb on - strand, within AO353_23395 at 5128.351 kb on - strand, within AO353_23395 at 5128.489 kb on - strand at 5128.530 kb on - strand at 5128.543 kb on + strand at 5128.543 kb on + strand at 5128.550 kb on - strand at 5128.834 kb on + strand, within AO353_23400 at 5128.834 kb on + strand, within AO353_23400 at 5128.834 kb on + strand, within AO353_23400 at 5128.834 kb on + strand, within AO353_23400 at 5128.834 kb on + strand, within AO353_23400 at 5128.883 kb on - strand, within AO353_23400 at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.959 kb on + strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5128.960 kb on - strand at 5129.003 kb on - strand at 5129.036 kb on + strand at 5129.081 kb on + strand at 5129.081 kb on + strand at 5129.082 kb on - strand at 5129.092 kb on + strand at 5129.093 kb on - strand at 5129.105 kb on + strand at 5129.105 kb on + strand at 5129.106 kb on - strand at 5129.106 kb on - strand at 5129.278 kb on + strand, within AO353_23405 at 5129.333 kb on + strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.384 kb on + strand at 5129.433 kb on + strand at 5129.433 kb on + strand at 5129.439 kb on + strand at 5129.440 kb on - strand at 5129.440 kb on - strand at 5129.549 kb on - strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.651 kb on - strand, within AO353_23410 at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.849 kb on + strand at 5129.849 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.926 kb on - strand at 5129.933 kb on + strand at 5129.933 kb on + strand at 5129.934 kb on - strand at 5129.934 kb on - strand at 5129.948 kb on - strand at 5129.948 kb on - strand at 5129.975 kb on + strand at 5129.976 kb on - strand at 5129.976 kb on - strand at 5130.114 kb on + strand, within AO353_23415 at 5130.114 kb on + strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.180 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 5,128,163 + AO353_23395 0.47 -0.1 5,128,164 - -0.7 5,128,229 + AO353_23395 0.58 -1.2 5,128,230 - AO353_23395 0.58 +0.4 5,128,271 - AO353_23395 0.64 -1.0 5,128,331 + AO353_23395 0.74 -1.1 5,128,331 + AO353_23395 0.74 -0.4 5,128,332 - AO353_23395 0.74 +0.0 5,128,350 + AO353_23395 0.77 +1.1 5,128,350 + AO353_23395 0.77 -2.1 5,128,351 - AO353_23395 0.77 -0.6 5,128,351 - AO353_23395 0.77 +0.4 5,128,489 - -0.5 5,128,530 - -0.3 5,128,543 + -0.3 5,128,543 + -1.1 5,128,550 - -0.6 5,128,834 + AO353_23400 0.59 -1.9 5,128,834 + AO353_23400 0.59 -0.7 5,128,834 + AO353_23400 0.59 -0.6 5,128,834 + AO353_23400 0.59 +1.5 5,128,834 + AO353_23400 0.59 -0.9 5,128,883 - AO353_23400 0.74 -2.2 5,128,959 + -0.1 5,128,959 + +2.1 5,128,959 + +1.4 5,128,959 + +1.3 5,128,959 + +0.3 5,128,959 + +2.3 5,128,959 + +0.3 5,128,959 + -1.1 5,128,959 + +1.3 5,128,959 + +0.3 5,128,959 + +0.6 5,128,959 + -0.3 5,128,959 + +0.5 5,128,960 - +0.5 5,128,960 - -0.1 5,128,960 - -0.1 5,128,960 - -0.5 5,128,960 - -0.1 5,128,960 - +0.8 5,128,960 - +1.0 5,128,960 - -1.3 5,128,960 - +1.6 5,128,960 - +0.7 5,128,960 - +1.1 5,128,960 - -0.7 5,128,960 - +0.2 5,128,960 - -0.2 5,129,003 - +1.0 5,129,036 + +0.1 5,129,081 + -0.1 5,129,081 + +0.8 5,129,082 - -0.1 5,129,092 + +1.3 5,129,093 - -0.7 5,129,105 + -0.4 5,129,105 + +1.1 5,129,106 - -0.3 5,129,106 - -1.2 5,129,278 + AO353_23405 0.58 +0.3 5,129,333 + AO353_23405 0.85 -0.5 5,129,334 - AO353_23405 0.86 -1.7 5,129,334 - AO353_23405 0.86 +0.7 5,129,334 - AO353_23405 0.86 -2.4 5,129,334 - AO353_23405 0.86 -0.3 5,129,384 + +0.9 5,129,433 + +0.3 5,129,433 + -1.1 5,129,439 + +0.7 5,129,440 - -0.2 5,129,440 - +0.1 5,129,549 - AO353_23410 0.41 +1.3 5,129,650 + AO353_23410 0.76 +1.5 5,129,650 + AO353_23410 0.76 +0.2 5,129,651 - AO353_23410 0.77 -0.1 5,129,701 + +0.0 5,129,701 + -0.3 5,129,701 + +0.9 5,129,701 + -2.0 5,129,701 + +0.3 5,129,701 + +0.3 5,129,701 + +0.0 5,129,702 - +0.2 5,129,702 - -0.6 5,129,702 - +0.0 5,129,849 + -2.1 5,129,849 + +0.9 5,129,852 + +0.5 5,129,852 + -0.9 5,129,852 + -0.6 5,129,852 + +0.3 5,129,852 + +0.7 5,129,853 - +0.2 5,129,853 - -1.1 5,129,853 - -0.5 5,129,853 - -0.0 5,129,853 - -1.5 5,129,853 - +0.7 5,129,925 + +0.3 5,129,925 + -0.7 5,129,925 + +0.7 5,129,925 + +0.3 5,129,925 + -1.4 5,129,925 + +0.5 5,129,926 - -0.9 5,129,933 + +1.5 5,129,933 + +0.3 5,129,934 - +1.3 5,129,934 - +0.7 5,129,948 - +0.1 5,129,948 - -2.1 5,129,975 + -0.7 5,129,976 - +0.4 5,129,976 - -1.1 5,130,114 + AO353_23415 0.21 -0.1 5,130,114 + AO353_23415 0.21 +0.8 5,130,115 - AO353_23415 0.21 +0.7 5,130,115 - AO353_23415 0.21 -1.7 5,130,115 - AO353_23415 0.21 -0.7 5,130,180 + AO353_23415 0.32 +0.9 5,130,314 + AO353_23415 0.55 -0.7 5,130,314 + AO353_23415 0.55 +1.6 5,130,337 - AO353_23415 0.59 +1.1 5,130,337 - AO353_23415 0.59 +0.6
Or see this region's nucleotide sequence