Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_04350 and AO353_04355 are separated by 66 nucleotides AO353_04355 and AO353_04360 are separated by 136 nucleotides
AO353_04350: AO353_04350 - threonine synthase, at 937,802 to 939,211
_04350
AO353_04355: AO353_04355 - homoserine dehydrogenase, at 939,278 to 940,582
_04355
AO353_04360: AO353_04360 - protein-disulfide isomerase, at 940,719 to 941,450
_04360
Position (kb)
939
940
941 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 938.512 kb on - strand, within AO353_04350 at 939.225 kb on - strand at 939.225 kb on - strand at 939.271 kb on + strand at 939.279 kb on + strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.699 kb on + strand, within AO353_04355 at 939.830 kb on - strand, within AO353_04355 at 940.358 kb on + strand, within AO353_04355 at 940.566 kb on - strand at 940.653 kb on - strand at 940.653 kb on - strand at 940.653 kb on - strand at 940.655 kb on + strand at 940.656 kb on - strand at 940.656 kb on - strand at 940.721 kb on - strand at 940.809 kb on - strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 941.076 kb on + strand, within AO353_04360 at 941.076 kb on + strand, within AO353_04360 at 941.077 kb on - strand, within AO353_04360 at 941.077 kb on - strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.258 kb on - strand, within AO353_04360 at 941.290 kb on + strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.316 kb on - strand, within AO353_04360 at 941.316 kb on - strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.361 kb on - strand, within AO353_04360 at 941.372 kb on + strand, within AO353_04360 at 941.372 kb on + strand, within AO353_04360 at 941.373 kb on - strand, within AO353_04360 at 941.483 kb on + strand at 941.483 kb on + strand at 941.484 kb on - strand at 941.484 kb on - strand at 941.542 kb on + strand at 941.542 kb on + strand at 941.542 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.550 kb on - strand at 941.550 kb on - strand at 941.550 kb on - strand at 941.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 938,512 - AO353_04350 0.50 -0.5 939,225 - -0.3 939,225 - -0.1 939,271 + -2.4 939,279 + +3.4 939,280 - -0.8 939,280 - +1.0 939,280 - +0.9 939,280 - +0.4 939,280 - -1.7 939,280 - +2.1 939,699 + AO353_04355 0.32 +0.0 939,830 - AO353_04355 0.42 +0.2 940,358 + AO353_04355 0.83 -0.4 940,566 - -1.4 940,653 - -0.3 940,653 - +0.5 940,653 - +0.6 940,655 + -0.5 940,656 - +1.0 940,656 - -1.0 940,721 - -1.0 940,809 - AO353_04360 0.12 +3.1 940,993 + AO353_04360 0.37 -0.2 940,993 + AO353_04360 0.37 +1.0 940,993 + AO353_04360 0.37 -0.9 940,993 + AO353_04360 0.37 +0.0 940,994 - AO353_04360 0.38 -0.4 940,994 - AO353_04360 0.38 -2.7 940,994 - AO353_04360 0.38 -0.1 941,076 + AO353_04360 0.49 -0.7 941,076 + AO353_04360 0.49 -0.5 941,077 - AO353_04360 0.49 -0.7 941,077 - AO353_04360 0.49 +0.3 941,221 + AO353_04360 0.69 +0.9 941,221 + AO353_04360 0.69 -1.0 941,221 + AO353_04360 0.69 -0.6 941,221 + AO353_04360 0.69 +1.8 941,221 + AO353_04360 0.69 +0.1 941,222 - AO353_04360 0.69 -0.8 941,222 - AO353_04360 0.69 -0.5 941,222 - AO353_04360 0.69 +0.7 941,222 - AO353_04360 0.69 -0.5 941,236 + AO353_04360 0.71 +0.0 941,236 + AO353_04360 0.71 -2.1 941,236 + AO353_04360 0.71 -1.5 941,236 + AO353_04360 0.71 -1.7 941,236 + AO353_04360 0.71 +1.2 941,236 + AO353_04360 0.71 +0.2 941,237 - AO353_04360 0.71 +1.1 941,237 - AO353_04360 0.71 -0.7 941,237 - AO353_04360 0.71 +0.8 941,258 - AO353_04360 0.74 -0.1 941,290 + AO353_04360 0.78 +0.5 941,291 - AO353_04360 0.78 +0.5 941,291 - AO353_04360 0.78 +0.3 941,291 - AO353_04360 0.78 +0.8 941,316 - AO353_04360 0.82 -0.6 941,316 - AO353_04360 0.82 +0.8 941,360 + AO353_04360 0.88 +0.6 941,360 + AO353_04360 0.88 +0.3 941,360 + AO353_04360 0.88 +1.2 941,361 - AO353_04360 0.88 -0.8 941,372 + AO353_04360 0.89 +0.5 941,372 + AO353_04360 0.89 -0.8 941,373 - AO353_04360 0.89 -0.5 941,483 + +0.0 941,483 + +0.1 941,484 - +1.0 941,484 - +0.5 941,542 + -0.2 941,542 + -0.4 941,542 + -1.0 941,549 + -0.1 941,549 + +0.5 941,549 + -1.0 941,549 + -0.2 941,550 - -1.8 941,550 - -0.8 941,550 - +2.8 941,550 - +0.4
Or see this region's nucleotide sequence