Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02995 and AO353_03000 are separated by 12 nucleotides AO353_03000 and AO353_03005 are separated by 63 nucleotides
AO353_02995: AO353_02995 - succinylglutamate desuccinylase, at 635,209 to 636,219
_02995
AO353_03000: AO353_03000 - topoisomerase II, at 636,232 to 636,519
_03000
AO353_03005: AO353_03005 - succinylarginine dihydrolase, at 636,583 to 637,929
_03005
Position (kb) 636
637 Strain fitness (log2 ratio) -2
-1
0
1 at 635.351 kb on + strand, within AO353_02995 at 635.352 kb on - strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.615 kb on + strand, within AO353_02995 at 635.616 kb on - strand, within AO353_02995 at 635.616 kb on - strand, within AO353_02995 at 635.726 kb on + strand, within AO353_02995 at 635.777 kb on + strand, within AO353_02995 at 635.778 kb on - strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 636.017 kb on - strand, within AO353_02995 at 636.017 kb on - strand, within AO353_02995 at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.220 kb on - strand at 636.220 kb on - strand at 636.313 kb on + strand, within AO353_03000 at 636.313 kb on + strand, within AO353_03000 at 636.314 kb on - strand, within AO353_03000 at 636.314 kb on - strand, within AO353_03000 at 636.322 kb on - strand, within AO353_03000 at 636.523 kb on + strand at 636.541 kb on + strand at 636.541 kb on + strand at 636.541 kb on + strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.603 kb on - strand at 636.619 kb on - strand at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.808 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.912 kb on - strand, within AO353_03005 at 637.004 kb on + strand, within AO353_03005 at 637.004 kb on + strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.139 kb on + strand, within AO353_03005 at 637.139 kb on + strand, within AO353_03005 at 637.140 kb on - strand, within AO353_03005 at 637.298 kb on + strand, within AO353_03005
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 635,351 + AO353_02995 0.14 -0.9 635,352 - AO353_02995 0.14 -1.1 635,609 + AO353_02995 0.40 -1.2 635,609 + AO353_02995 0.40 -0.5 635,609 + AO353_02995 0.40 +0.2 635,610 - AO353_02995 0.40 -0.4 635,610 - AO353_02995 0.40 -0.6 635,610 - AO353_02995 0.40 -0.5 635,610 - AO353_02995 0.40 -1.3 635,610 - AO353_02995 0.40 +1.3 635,615 + AO353_02995 0.40 -0.5 635,616 - AO353_02995 0.40 -0.1 635,616 - AO353_02995 0.40 -2.0 635,726 + AO353_02995 0.51 -0.5 635,777 + AO353_02995 0.56 -0.1 635,778 - AO353_02995 0.56 +0.2 635,961 + AO353_02995 0.74 -0.9 635,961 + AO353_02995 0.74 -1.2 635,961 + AO353_02995 0.74 -2.0 635,961 + AO353_02995 0.74 -1.0 635,961 + AO353_02995 0.74 -0.2 635,961 + AO353_02995 0.74 -0.7 635,962 - AO353_02995 0.74 +1.2 635,962 - AO353_02995 0.74 -0.2 635,962 - AO353_02995 0.74 +0.6 636,017 - AO353_02995 0.80 +0.3 636,017 - AO353_02995 0.80 +1.7 636,219 + -0.5 636,219 + -0.2 636,219 + -0.6 636,219 + -0.7 636,219 + -0.3 636,219 + -1.5 636,219 + +0.4 636,220 - -2.1 636,220 - +0.1 636,313 + AO353_03000 0.28 -0.7 636,313 + AO353_03000 0.28 -1.6 636,314 - AO353_03000 0.28 -0.8 636,314 - AO353_03000 0.28 -1.7 636,322 - AO353_03000 0.31 +0.1 636,523 + -1.3 636,541 + +0.7 636,541 + -0.9 636,541 + +0.1 636,542 - -0.0 636,542 - +0.5 636,542 - -0.8 636,542 - +0.1 636,542 - -0.9 636,542 - -1.0 636,603 - +0.8 636,619 - +0.5 636,741 - AO353_03005 0.12 +0.1 636,741 - AO353_03005 0.12 -0.1 636,741 - AO353_03005 0.12 +0.1 636,741 - AO353_03005 0.12 -0.1 636,741 - AO353_03005 0.12 +1.2 636,741 - AO353_03005 0.12 +1.2 636,808 + AO353_03005 0.17 -0.6 636,911 + AO353_03005 0.24 +1.7 636,911 + AO353_03005 0.24 +1.4 636,911 + AO353_03005 0.24 +0.9 636,912 - AO353_03005 0.24 -0.3 637,004 + AO353_03005 0.31 +0.7 637,004 + AO353_03005 0.31 -0.5 637,005 - AO353_03005 0.31 +0.4 637,005 - AO353_03005 0.31 +0.7 637,005 - AO353_03005 0.31 -0.4 637,005 - AO353_03005 0.31 +0.6 637,139 + AO353_03005 0.41 +1.0 637,139 + AO353_03005 0.41 -0.3 637,140 - AO353_03005 0.41 +0.4 637,298 + AO353_03005 0.53 +0.9
Or see this region's nucleotide sequence