Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2880 and DvMF_2881 overlap by 59 nucleotides DvMF_2881 and DvMF_2882 overlap by 4 nucleotides DvMF_2882 and rplS are separated by 276 nucleotides rplS and DvMF_2884 are separated by 55 nucleotides
DvMF_2880: DvMF_2880 - Uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase (RefSeq), at 3,637,895 to 3,638,734
_2880
DvMF_2881: DvMF_2881 - protein of unknown function UPF0102 (RefSeq), at 3,638,676 to 3,639,116
_2881
DvMF_2882: DvMF_2882 - Ribonuclease H (RefSeq), at 3,639,113 to 3,639,991
_2882
DvMF_2883: rplS - 50S ribosomal protein L19 (RefSeq), at 3,640,268 to 3,640,615
rplS
DvMF_2884: DvMF_2884 - tRNA (guanine-N1)-methyltransferase (RefSeq), at 3,640,671 to 3,641,966
_2884
Position (kb)
3639
3640 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3638.184 kb on + strand, within DvMF_2880 at 3638.188 kb on + strand, within DvMF_2880 at 3638.217 kb on + strand, within DvMF_2880 at 3638.274 kb on - strand, within DvMF_2880 at 3638.368 kb on - strand, within DvMF_2880 at 3638.378 kb on - strand, within DvMF_2880 at 3638.413 kb on + strand, within DvMF_2880 at 3638.546 kb on - strand, within DvMF_2880 at 3638.707 kb on + strand at 3638.740 kb on - strand, within DvMF_2881 at 3638.766 kb on + strand, within DvMF_2881 at 3638.869 kb on - strand, within DvMF_2881 at 3638.913 kb on + strand, within DvMF_2881 at 3639.120 kb on + strand at 3639.215 kb on - strand, within DvMF_2882 at 3639.232 kb on - strand, within DvMF_2882 at 3639.279 kb on + strand, within DvMF_2882 at 3639.282 kb on - strand, within DvMF_2882 at 3639.356 kb on - strand, within DvMF_2882
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 3,638,184 + DvMF_2880 0.34 -2.0 3,638,188 + DvMF_2880 0.35 -0.2 3,638,217 + DvMF_2880 0.38 -0.9 3,638,274 - DvMF_2880 0.45 -0.6 3,638,368 - DvMF_2880 0.56 +0.1 3,638,378 - DvMF_2880 0.57 -5.3 3,638,413 + DvMF_2880 0.62 -1.1 3,638,546 - DvMF_2880 0.78 -0.8 3,638,707 + -1.1 3,638,740 - DvMF_2881 0.15 -0.7 3,638,766 + DvMF_2881 0.20 -0.4 3,638,869 - DvMF_2881 0.44 -1.5 3,638,913 + DvMF_2881 0.54 -0.5 3,639,120 + -3.6 3,639,215 - DvMF_2882 0.12 -0.6 3,639,232 - DvMF_2882 0.14 -1.0 3,639,279 + DvMF_2882 0.19 -2.7 3,639,282 - DvMF_2882 0.19 -0.1 3,639,356 - DvMF_2882 0.28 -1.2
Or see this region's nucleotide sequence