Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2461 and DvMF_2462 are separated by 314 nucleotides DvMF_2462 and DvMF_2463 are separated by 44 nucleotides DvMF_2463 and DvMF_2464 are separated by 432 nucleotides
DvMF_2461: DvMF_2461 - fumarate hydratase (RefSeq), at 3,104,594 to 3,105,433
_2461
DvMF_2462: DvMF_2462 - hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit (RefSeq), at 3,105,748 to 3,106,275
_2462
DvMF_2463: DvMF_2463 - hypothetical protein (RefSeq), at 3,106,320 to 3,107,615
_2463
DvMF_2464: DvMF_2464 - hypothetical protein (RefSeq), at 3,108,048 to 3,108,530
_2464
Position (kb)
3106
3107
3108 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3105.343 kb on - strand, within DvMF_2461 at 3105.348 kb on + strand, within DvMF_2461 at 3105.555 kb on + strand at 3105.767 kb on + strand at 3105.834 kb on + strand, within DvMF_2462 at 3105.834 kb on + strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.868 kb on - strand, within DvMF_2462 at 3105.902 kb on + strand, within DvMF_2462 at 3105.972 kb on - strand, within DvMF_2462 at 3106.013 kb on - strand, within DvMF_2462 at 3106.085 kb on + strand, within DvMF_2462 at 3106.093 kb on - strand, within DvMF_2462 at 3106.311 kb on + strand at 3106.336 kb on + strand at 3106.618 kb on + strand, within DvMF_2463 at 3106.636 kb on + strand, within DvMF_2463 at 3106.636 kb on + strand, within DvMF_2463 at 3106.664 kb on + strand, within DvMF_2463 at 3106.688 kb on - strand, within DvMF_2463 at 3106.703 kb on + strand, within DvMF_2463 at 3106.934 kb on - strand, within DvMF_2463 at 3107.078 kb on + strand, within DvMF_2463 at 3107.101 kb on - strand, within DvMF_2463 at 3107.106 kb on - strand, within DvMF_2463 at 3107.225 kb on - strand, within DvMF_2463 at 3107.663 kb on + strand at 3107.671 kb on - strand at 3107.704 kb on - strand at 3107.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 3,105,343 - DvMF_2461 0.89 -0.9 3,105,348 + DvMF_2461 0.90 -1.4 3,105,555 + -0.2 3,105,767 + -0.1 3,105,834 + DvMF_2462 0.16 -0.5 3,105,834 + DvMF_2462 0.16 -1.0 3,105,842 - DvMF_2462 0.18 -0.3 3,105,842 - DvMF_2462 0.18 -0.7 3,105,842 - DvMF_2462 0.18 -0.2 3,105,868 - DvMF_2462 0.23 -0.4 3,105,902 + DvMF_2462 0.29 -0.6 3,105,972 - DvMF_2462 0.42 +0.3 3,106,013 - DvMF_2462 0.50 -0.4 3,106,085 + DvMF_2462 0.64 -0.4 3,106,093 - DvMF_2462 0.65 -1.5 3,106,311 + +0.2 3,106,336 + -0.9 3,106,618 + DvMF_2463 0.23 -1.5 3,106,636 + DvMF_2463 0.24 -2.0 3,106,636 + DvMF_2463 0.24 -0.6 3,106,664 + DvMF_2463 0.27 -1.5 3,106,688 - DvMF_2463 0.28 -0.8 3,106,703 + DvMF_2463 0.30 -0.7 3,106,934 - DvMF_2463 0.47 -0.5 3,107,078 + DvMF_2463 0.58 -1.5 3,107,101 - DvMF_2463 0.60 -1.4 3,107,106 - DvMF_2463 0.61 -0.7 3,107,225 - DvMF_2463 0.70 -3.1 3,107,663 + +0.3 3,107,671 - +1.3 3,107,704 - -1.2 3,107,877 - -1.0
Or see this region's nucleotide sequence