Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1576 and DvMF_1577 are separated by 170 nucleotides DvMF_1577 and DvMF_R0045 are separated by 200 nucleotides DvMF_R0045 and DvMF_R0046 are separated by 99 nucleotides DvMF_R0046 and DvMF_R0047 are separated by 6 nucleotides
DvMF_1576: DvMF_1576 - transcriptional regulator, AraC family (RefSeq), at 1,944,294 to 1,945,604
_1576
DvMF_1577: DvMF_1577 - hypothetical protein (RefSeq), at 1,945,775 to 1,946,011
_1577
DvMF_R0045: DvMF_R0045 - tRNA-Asp (RefSeq), at 1,946,212 to 1,946,288
_R0045
DvMF_R0046: DvMF_R0046 - tRNA-Asp (RefSeq), at 1,946,388 to 1,946,464
_R0046
DvMF_R0047: DvMF_R0047 - tRNA-Val (RefSeq), at 1,946,471 to 1,946,546
_R0047
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1944.806 kb on - strand, within DvMF_1576 at 1944.870 kb on - strand, within DvMF_1576 at 1944.939 kb on - strand, within DvMF_1576 at 1945.187 kb on + strand, within DvMF_1576 at 1945.638 kb on + strand at 1945.705 kb on - strand at 1945.768 kb on + strand at 1945.771 kb on - strand at 1945.894 kb on + strand, within DvMF_1577 at 1945.967 kb on + strand, within DvMF_1577 at 1946.070 kb on + strand at 1946.073 kb on - strand at 1946.075 kb on + strand at 1946.075 kb on + strand at 1946.083 kb on - strand at 1946.083 kb on - strand at 1946.713 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 1,944,806 - DvMF_1576 0.39 +0.4 1,944,870 - DvMF_1576 0.44 +0.4 1,944,939 - DvMF_1576 0.49 -0.2 1,945,187 + DvMF_1576 0.68 -0.6 1,945,638 + -2.8 1,945,705 - -0.3 1,945,768 + +1.5 1,945,771 - -2.7 1,945,894 + DvMF_1577 0.50 -3.6 1,945,967 + DvMF_1577 0.81 +0.3 1,946,070 + -0.8 1,946,073 - -0.5 1,946,075 + -0.1 1,946,075 + -0.9 1,946,083 - +0.1 1,946,083 - -1.5 1,946,713 + -0.5
Or see this region's nucleotide sequence