Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0715 and DvMF_0716 overlap by 11 nucleotides DvMF_0716 and DvMF_0717 are separated by 1 nucleotides DvMF_0717 and DvMF_0718 are separated by 90 nucleotides DvMF_0718 and DvMF_0719 are separated by 2 nucleotides DvMF_0719 and DvMF_0720 overlap by 14 nucleotides DvMF_0720 and DvMF_0721 overlap by 4 nucleotides
DvMF_0715: DvMF_0715 - hypothetical protein (RefSeq), at 868,823 to 869,416
_0715
DvMF_0716: DvMF_0716 - regulatory protein, LacI (RefSeq), at 869,406 to 869,735
_0716
DvMF_0717: DvMF_0717 - hypothetical protein (RefSeq), at 869,737 to 870,231
_0717
DvMF_0718: DvMF_0718 - hypothetical protein (RefSeq), at 870,322 to 870,774
_0718
DvMF_0719: DvMF_0719 - hypothetical protein (RefSeq), at 870,777 to 871,001
_0719
DvMF_0720: DvMF_0720 - protein of unknown function DUF1018 (RefSeq), at 870,988 to 871,428
_0720
DvMF_0721: DvMF_0721 - Mor transcription activator domain protein (RefSeq), at 871,425 to 871,895
_0721
Position (kb) 870
871 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 869.322 kb on - strand, within DvMF_0715 at 870.104 kb on - strand, within DvMF_0717 at 870.127 kb on - strand, within DvMF_0717 at 870.394 kb on - strand, within DvMF_0718 at 870.652 kb on - strand, within DvMF_0718 at 870.772 kb on - strand at 870.794 kb on + strand at 871.108 kb on + strand, within DvMF_0720 at 871.185 kb on + strand, within DvMF_0720 at 871.190 kb on + strand, within DvMF_0720 at 871.619 kb on + strand, within DvMF_0721
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 869,322 - DvMF_0715 0.84 -2.9 870,104 - DvMF_0717 0.74 +1.4 870,127 - DvMF_0717 0.79 -1.3 870,394 - DvMF_0718 0.16 -1.0 870,652 - DvMF_0718 0.73 -3.2 870,772 - +0.5 870,794 + -2.5 871,108 + DvMF_0720 0.27 -3.3 871,185 + DvMF_0720 0.45 -0.7 871,190 + DvMF_0720 0.46 +1.2 871,619 + DvMF_0721 0.41 -1.8
Or see this region's nucleotide sequence