Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0677 and DvMF_0678 overlap by 4 nucleotides DvMF_0678 and DvMF_0679 overlap by 4 nucleotides
DvMF_0677: DvMF_0677 - glycosyl transferase group 1 (RefSeq), at 816,458 to 817,651
_0677
DvMF_0678: DvMF_0678 - polysaccharide biosynthesis protein (RefSeq), at 817,648 to 819,147
_0678
DvMF_0679: DvMF_0679 - O-antigen polymerase (RefSeq), at 819,144 to 820,457
_0679
Position (kb) 817
818
819
820 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 816.727 kb on + strand, within DvMF_0677 at 816.727 kb on + strand, within DvMF_0677 at 817.009 kb on - strand, within DvMF_0677 at 817.207 kb on - strand, within DvMF_0677 at 817.259 kb on + strand, within DvMF_0677 at 817.266 kb on + strand, within DvMF_0677 at 817.374 kb on - strand, within DvMF_0677 at 817.492 kb on + strand, within DvMF_0677 at 817.641 kb on + strand at 817.663 kb on - strand at 817.881 kb on + strand, within DvMF_0678 at 817.918 kb on + strand, within DvMF_0678 at 817.926 kb on - strand, within DvMF_0678 at 818.322 kb on + strand, within DvMF_0678 at 818.322 kb on + strand, within DvMF_0678 at 818.387 kb on + strand, within DvMF_0678 at 818.395 kb on - strand, within DvMF_0678 at 818.461 kb on - strand, within DvMF_0678 at 818.485 kb on + strand, within DvMF_0678 at 818.564 kb on - strand, within DvMF_0678 at 818.601 kb on + strand, within DvMF_0678 at 818.601 kb on + strand, within DvMF_0678 at 818.609 kb on - strand, within DvMF_0678 at 818.822 kb on + strand, within DvMF_0678 at 818.822 kb on + strand, within DvMF_0678 at 818.891 kb on + strand, within DvMF_0678 at 819.235 kb on + strand at 819.345 kb on - strand, within DvMF_0679 at 819.624 kb on + strand, within DvMF_0679 at 819.930 kb on - strand, within DvMF_0679
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 816,727 + DvMF_0677 0.23 -0.2 816,727 + DvMF_0677 0.23 -0.3 817,009 - DvMF_0677 0.46 -4.1 817,207 - DvMF_0677 0.63 -0.8 817,259 + DvMF_0677 0.67 -2.1 817,266 + DvMF_0677 0.68 -0.9 817,374 - DvMF_0677 0.77 -1.1 817,492 + DvMF_0677 0.87 -0.5 817,641 + +0.7 817,663 - -0.6 817,881 + DvMF_0678 0.16 -0.6 817,918 + DvMF_0678 0.18 -0.7 817,926 - DvMF_0678 0.19 -2.1 818,322 + DvMF_0678 0.45 -0.9 818,322 + DvMF_0678 0.45 -0.6 818,387 + DvMF_0678 0.49 -0.9 818,395 - DvMF_0678 0.50 +0.4 818,461 - DvMF_0678 0.54 -0.1 818,485 + DvMF_0678 0.56 -0.4 818,564 - DvMF_0678 0.61 -1.6 818,601 + DvMF_0678 0.64 -0.7 818,601 + DvMF_0678 0.64 -1.1 818,609 - DvMF_0678 0.64 -1.4 818,822 + DvMF_0678 0.78 -1.7 818,822 + DvMF_0678 0.78 -0.8 818,891 + DvMF_0678 0.83 -0.6 819,235 + -1.1 819,345 - DvMF_0679 0.15 -1.3 819,624 + DvMF_0679 0.37 -0.3 819,930 - DvMF_0679 0.60 -0.8
Or see this region's nucleotide sequence