Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0533 and DvMF_0534 are separated by 6 nucleotides DvMF_0534 and DvMF_0535 are separated by 9 nucleotides DvMF_0535 and DvMF_0536 overlap by 8 nucleotides
DvMF_0533: DvMF_0533 - cytochrome c oxidase, subunit II (RefSeq), at 629,143 to 630,360
_0533
DvMF_0534: DvMF_0534 - caa(3)-type oxidase, subunit IV (RefSeq), at 630,367 to 630,657
_0534
DvMF_0535: DvMF_0535 - cytochrome c oxidase subunit III (RefSeq), at 630,667 to 631,263
_0535
DvMF_0536: DvMF_0536 - cytochrome c oxidase, subunit I (RefSeq), at 631,256 to 632,881
_0536
Position (kb) 630
631
632 Strain fitness (log2 ratio) -1
0
1 at 630.123 kb on + strand, within DvMF_0533 at 630.444 kb on + strand, within DvMF_0534 at 630.590 kb on - strand, within DvMF_0534 at 630.596 kb on + strand, within DvMF_0534 at 630.730 kb on - strand, within DvMF_0535 at 630.815 kb on - strand, within DvMF_0535 at 630.828 kb on + strand, within DvMF_0535 at 631.174 kb on - strand, within DvMF_0535 at 631.353 kb on - strand at 631.629 kb on + strand, within DvMF_0536 at 632.184 kb on - strand, within DvMF_0536 at 632.235 kb on - strand, within DvMF_0536 at 632.245 kb on + strand, within DvMF_0536
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 630,123 + DvMF_0533 0.80 -0.4 630,444 + DvMF_0534 0.26 -0.6 630,590 - DvMF_0534 0.77 +0.4 630,596 + DvMF_0534 0.79 -0.6 630,730 - DvMF_0535 0.11 +0.2 630,815 - DvMF_0535 0.25 -1.5 630,828 + DvMF_0535 0.27 -1.5 631,174 - DvMF_0535 0.85 -0.8 631,353 - -0.5 631,629 + DvMF_0536 0.23 -0.5 632,184 - DvMF_0536 0.57 +0.2 632,235 - DvMF_0536 0.60 -0.4 632,245 + DvMF_0536 0.61 -0.8
Or see this region's nucleotide sequence