Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0519 and DvMF_0520 are separated by 69 nucleotides DvMF_0520 and DvMF_0521 are separated by 3 nucleotides DvMF_0521 and DvMF_0522 are separated by 136 nucleotides
DvMF_0519: DvMF_0519 - ABC-type nitrate/sulfonate/bicarbonate transport systems periplasmic components-like protein (RefSeq), at 612,678 to 613,742
_0519
DvMF_0520: DvMF_0520 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 613,812 to 614,609
_0520
DvMF_0521: DvMF_0521 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 614,613 to 615,491
_0521
DvMF_0522: DvMF_0522 - ABC transporter related (RefSeq), at 615,628 to 616,377
_0522
Position (kb) 614
615
616 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 613.615 kb on - strand, within DvMF_0519 at 613.616 kb on - strand, within DvMF_0519 at 613.658 kb on + strand at 613.732 kb on - strand at 613.924 kb on - strand, within DvMF_0520 at 614.460 kb on + strand, within DvMF_0520 at 614.525 kb on + strand, within DvMF_0520 at 614.673 kb on - strand at 614.704 kb on + strand, within DvMF_0521 at 614.720 kb on + strand, within DvMF_0521 at 614.811 kb on + strand, within DvMF_0521 at 614.966 kb on + strand, within DvMF_0521 at 615.085 kb on + strand, within DvMF_0521 at 615.293 kb on - strand, within DvMF_0521 at 615.336 kb on + strand, within DvMF_0521 at 615.355 kb on + strand, within DvMF_0521 at 615.614 kb on + strand at 615.962 kb on + strand, within DvMF_0522 at 615.972 kb on + strand, within DvMF_0522 at 615.990 kb on - strand, within DvMF_0522 at 616.037 kb on + strand, within DvMF_0522 at 616.206 kb on - strand, within DvMF_0522 at 616.224 kb on - strand, within DvMF_0522 at 616.229 kb on - strand, within DvMF_0522 at 616.250 kb on + strand, within DvMF_0522 at 616.358 kb on + strand at 616.415 kb on + strand at 616.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 613,615 - DvMF_0519 0.88 -1.0 613,616 - DvMF_0519 0.88 +0.3 613,658 + +0.9 613,732 - -1.0 613,924 - DvMF_0520 0.14 -3.2 614,460 + DvMF_0520 0.81 -1.0 614,525 + DvMF_0520 0.89 -1.2 614,673 - +1.8 614,704 + DvMF_0521 0.10 +0.4 614,720 + DvMF_0521 0.12 +0.1 614,811 + DvMF_0521 0.23 -0.1 614,966 + DvMF_0521 0.40 -1.0 615,085 + DvMF_0521 0.54 -0.5 615,293 - DvMF_0521 0.77 -1.0 615,336 + DvMF_0521 0.82 -0.7 615,355 + DvMF_0521 0.84 -1.8 615,614 + -0.5 615,962 + DvMF_0522 0.45 -0.8 615,972 + DvMF_0522 0.46 -0.7 615,990 - DvMF_0522 0.48 -1.5 616,037 + DvMF_0522 0.55 +0.2 616,206 - DvMF_0522 0.77 -2.7 616,224 - DvMF_0522 0.79 -2.9 616,229 - DvMF_0522 0.80 -2.4 616,250 + DvMF_0522 0.83 +0.0 616,358 + -0.8 616,415 + -0.1 616,417 - -0.2
Or see this region's nucleotide sequence