Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0208 and DvMF_0209 are separated by 30 nucleotides DvMF_0209 and DvMF_0210 are separated by 147 nucleotides
DvMF_0208: DvMF_0208 - EAL domain protein (RefSeq), at 227,131 to 229,419
_0208
DvMF_0209: DvMF_0209 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 229,450 to 230,418
_0209
DvMF_0210: DvMF_0210 - Extracellular ligand-binding receptor (RefSeq), at 230,566 to 232,344
_0210
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 228.526 kb on - strand, within DvMF_0208 at 228.578 kb on - strand, within DvMF_0208 at 228.720 kb on - strand, within DvMF_0208 at 229.152 kb on - strand, within DvMF_0208 at 229.191 kb on + strand at 229.199 kb on - strand at 229.342 kb on + strand at 229.455 kb on - strand at 229.525 kb on + strand at 229.543 kb on + strand at 229.590 kb on - strand, within DvMF_0209 at 229.737 kb on + strand, within DvMF_0209 at 229.766 kb on + strand, within DvMF_0209 at 229.806 kb on - strand, within DvMF_0209 at 230.125 kb on + strand, within DvMF_0209 at 230.396 kb on - strand at 230.406 kb on - strand at 230.592 kb on - strand at 230.634 kb on - strand at 230.855 kb on - strand, within DvMF_0210 at 230.986 kb on + strand, within DvMF_0210 at 231.100 kb on - strand, within DvMF_0210 at 231.156 kb on - strand, within DvMF_0210 at 231.315 kb on + strand, within DvMF_0210
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 228,526 - DvMF_0208 0.61 +0.3 228,578 - DvMF_0208 0.63 -0.6 228,720 - DvMF_0208 0.69 -0.8 229,152 - DvMF_0208 0.88 -0.5 229,191 + -0.4 229,199 - +0.6 229,342 + -3.3 229,455 - -4.2 229,525 + +0.0 229,543 + -0.7 229,590 - DvMF_0209 0.14 -1.2 229,737 + DvMF_0209 0.30 -1.5 229,766 + DvMF_0209 0.33 -0.5 229,806 - DvMF_0209 0.37 -0.1 230,125 + DvMF_0209 0.70 -1.0 230,396 - -0.7 230,406 - -0.1 230,592 - -0.7 230,634 - -0.7 230,855 - DvMF_0210 0.16 -0.9 230,986 + DvMF_0210 0.24 -0.4 231,100 - DvMF_0210 0.30 -4.3 231,156 - DvMF_0210 0.33 -0.6 231,315 + DvMF_0210 0.42 -0.9
Or see this region's nucleotide sequence