Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0162 and DvMF_0163 are separated by 335 nucleotides DvMF_0163 and DvMF_0164 are separated by 81 nucleotides DvMF_0164 and DvMF_0165 are separated by 121 nucleotides DvMF_0165 and DvMF_0166 are separated by 11 nucleotides
DvMF_0162: DvMF_0162 - hypothetical protein (RefSeq), at 176,913 to 178,202
_0162
DvMF_0163: DvMF_0163 - no description, at 178,538 to 178,755
_0163
DvMF_0164: DvMF_0164 - hypothetical protein (RefSeq), at 178,837 to 179,658
_0164
DvMF_0165: DvMF_0165 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 179,780 to 180,337
_0165
DvMF_0166: DvMF_0166 - Formate dehydrogenase (RefSeq), at 180,349 to 182,484
_0166
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 177.871 kb on - strand, within DvMF_0162 at 177.940 kb on + strand, within DvMF_0162 at 177.948 kb on - strand, within DvMF_0162 at 177.976 kb on + strand, within DvMF_0162 at 177.984 kb on - strand, within DvMF_0162 at 177.984 kb on - strand, within DvMF_0162 at 178.104 kb on - strand at 178.291 kb on - strand at 178.319 kb on + strand at 178.415 kb on + strand at 178.591 kb on + strand, within DvMF_0163 at 178.591 kb on + strand, within DvMF_0163 at 178.591 kb on + strand, within DvMF_0163 at 178.599 kb on - strand, within DvMF_0163 at 178.788 kb on + strand at 178.796 kb on - strand at 178.796 kb on - strand at 178.833 kb on - strand at 178.853 kb on - strand at 178.882 kb on - strand at 178.962 kb on + strand, within DvMF_0164 at 179.071 kb on - strand, within DvMF_0164 at 179.499 kb on + strand, within DvMF_0164 at 179.523 kb on + strand, within DvMF_0164 at 180.066 kb on + strand, within DvMF_0165 at 180.110 kb on - strand, within DvMF_0165 at 180.407 kb on - strand at 180.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 177,871 - DvMF_0162 0.74 -1.1 177,940 + DvMF_0162 0.80 +0.4 177,948 - DvMF_0162 0.80 +0.3 177,976 + DvMF_0162 0.82 -1.9 177,984 - DvMF_0162 0.83 +0.5 177,984 - DvMF_0162 0.83 -0.3 178,104 - -3.2 178,291 - -0.2 178,319 + -1.8 178,415 + +0.4 178,591 + DvMF_0163 0.24 -0.0 178,591 + DvMF_0163 0.24 -0.8 178,591 + DvMF_0163 0.24 -1.6 178,599 - DvMF_0163 0.28 -0.3 178,788 + -1.1 178,796 - -0.6 178,796 - -0.9 178,833 - +1.1 178,853 - -0.7 178,882 - -0.6 178,962 + DvMF_0164 0.15 -0.8 179,071 - DvMF_0164 0.28 -1.7 179,499 + DvMF_0164 0.81 -0.6 179,523 + DvMF_0164 0.83 -0.6 180,066 + DvMF_0165 0.51 +1.4 180,110 - DvMF_0165 0.59 -1.1 180,407 - +1.3 180,502 + -0.7
Or see this region's nucleotide sequence