Experiment: MoYLS4 with A22 0.0875 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0158 and DvMF_0159 are separated by 247 nucleotides DvMF_0159 and DvMF_0160 are separated by 220 nucleotides DvMF_0160 and DvMF_0161 are separated by 151 nucleotides
DvMF_0158: DvMF_0158 - HesB-like domain-containing protein (RefSeq), at 172,600 to 172,920
_0158
DvMF_0159: DvMF_0159 - HesB-like domain-containing protein (RefSeq), at 173,168 to 173,461
_0159
DvMF_0160: DvMF_0160 - bifunctional pyrimidine regulatory protein PyrR uracil phosphoribosyltransferase (RefSeq), at 173,682 to 174,215
_0160
DvMF_0161: DvMF_0161 - integral membrane protein (RefSeq), at 174,367 to 176,121
_0161
Position (kb) 173
174
175 Strain fitness (log2 ratio) -2
-1
0
1 at 172.781 kb on + strand, within DvMF_0158 at 172.995 kb on + strand at 173.018 kb on - strand at 173.028 kb on + strand at 173.235 kb on + strand, within DvMF_0159 at 173.295 kb on + strand, within DvMF_0159 at 173.577 kb on - strand at 173.872 kb on - strand, within DvMF_0160 at 173.872 kb on - strand, within DvMF_0160 at 174.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with A22 0.0875 mg/ml remove 172,781 + DvMF_0158 0.56 -0.3 172,995 + +0.1 173,018 - -0.2 173,028 + -0.8 173,235 + DvMF_0159 0.23 -0.1 173,295 + DvMF_0159 0.43 -2.7 173,577 - -1.0 173,872 - DvMF_0160 0.36 -1.1 173,872 - DvMF_0160 0.36 -0.9 174,218 - +0.0
Or see this region's nucleotide sequence