Experiment: BHIS with Chloride 350 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4404 and BT4405 are separated by 7 nucleotides BT4405 and BT4406 are separated by 3 nucleotides
BT4404: BT4404 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,800,675 to 5,803,995
BT4404
BT4405: BT4405 - hypothetical protein (NCBI ptt file), at 5,804,003 to 5,805,631
BT4405
BT4406: BT4406 - hypothetical protein (NCBI ptt file), at 5,805,635 to 5,806,801
BT4406
Position (kb)
5804
5805
5806 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5803.015 kb on - strand, within BT4404 at 5803.074 kb on + strand, within BT4404 at 5803.079 kb on - strand, within BT4404 at 5803.084 kb on - strand, within BT4404 at 5803.097 kb on - strand, within BT4404 at 5803.097 kb on - strand, within BT4404 at 5803.151 kb on - strand, within BT4404 at 5803.165 kb on - strand, within BT4404 at 5803.332 kb on - strand, within BT4404 at 5803.384 kb on - strand, within BT4404 at 5803.385 kb on + strand, within BT4404 at 5803.387 kb on + strand, within BT4404 at 5803.388 kb on - strand, within BT4404 at 5803.395 kb on + strand, within BT4404 at 5803.623 kb on - strand, within BT4404 at 5803.665 kb on - strand at 5803.703 kb on - strand at 5803.750 kb on + strand at 5803.836 kb on + strand at 5803.837 kb on - strand at 5803.922 kb on - strand at 5803.940 kb on - strand at 5803.993 kb on + strand at 5803.994 kb on - strand at 5804.022 kb on - strand at 5804.121 kb on + strand at 5804.232 kb on - strand, within BT4405 at 5804.232 kb on - strand, within BT4405 at 5804.276 kb on - strand, within BT4405 at 5804.279 kb on - strand, within BT4405 at 5804.288 kb on - strand, within BT4405 at 5804.302 kb on + strand, within BT4405 at 5804.306 kb on - strand, within BT4405 at 5804.310 kb on - strand, within BT4405 at 5804.343 kb on - strand, within BT4405 at 5804.355 kb on - strand, within BT4405 at 5804.524 kb on - strand, within BT4405 at 5804.644 kb on - strand, within BT4405 at 5804.645 kb on + strand, within BT4405 at 5804.675 kb on + strand, within BT4405 at 5804.711 kb on - strand, within BT4405 at 5804.829 kb on + strand, within BT4405 at 5804.836 kb on + strand, within BT4405 at 5805.071 kb on - strand, within BT4405 at 5805.223 kb on + strand, within BT4405 at 5805.276 kb on - strand, within BT4405 at 5805.285 kb on - strand, within BT4405 at 5805.301 kb on + strand, within BT4405 at 5805.301 kb on + strand, within BT4405 at 5805.493 kb on + strand at 5805.501 kb on - strand at 5805.501 kb on - strand at 5805.592 kb on + strand at 5805.666 kb on + strand at 5805.668 kb on + strand at 5805.668 kb on + strand at 5805.670 kb on + strand at 5805.684 kb on + strand at 5805.779 kb on + strand, within BT4406 at 5806.001 kb on - strand, within BT4406 at 5806.030 kb on + strand, within BT4406 at 5806.140 kb on - strand, within BT4406 at 5806.292 kb on + strand, within BT4406 at 5806.297 kb on - strand, within BT4406 at 5806.410 kb on - strand, within BT4406 at 5806.416 kb on - strand, within BT4406 at 5806.466 kb on + strand, within BT4406 at 5806.494 kb on - strand, within BT4406 at 5806.551 kb on - strand, within BT4406 at 5806.564 kb on + strand, within BT4406
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chloride 350 mM remove 5,803,015 - BT4404 0.70 +0.6 5,803,074 + BT4404 0.72 -0.6 5,803,079 - BT4404 0.72 +1.5 5,803,084 - BT4404 0.73 +1.8 5,803,097 - BT4404 0.73 +0.8 5,803,097 - BT4404 0.73 +0.2 5,803,151 - BT4404 0.75 +2.7 5,803,165 - BT4404 0.75 +1.5 5,803,332 - BT4404 0.80 -2.4 5,803,384 - BT4404 0.82 -0.8 5,803,385 + BT4404 0.82 +0.7 5,803,387 + BT4404 0.82 -1.3 5,803,388 - BT4404 0.82 -0.1 5,803,395 + BT4404 0.82 -1.6 5,803,623 - BT4404 0.89 -2.8 5,803,665 - -2.1 5,803,703 - -3.1 5,803,750 + -1.3 5,803,836 + -1.5 5,803,837 - -1.6 5,803,922 - -0.9 5,803,940 - -2.2 5,803,993 + +1.0 5,803,994 - +0.5 5,804,022 - +0.9 5,804,121 + +0.9 5,804,232 - BT4405 0.14 -1.8 5,804,232 - BT4405 0.14 +0.3 5,804,276 - BT4405 0.17 -1.2 5,804,279 - BT4405 0.17 +1.2 5,804,288 - BT4405 0.17 -1.5 5,804,302 + BT4405 0.18 -1.6 5,804,306 - BT4405 0.19 -2.2 5,804,310 - BT4405 0.19 -1.0 5,804,343 - BT4405 0.21 -0.1 5,804,355 - BT4405 0.22 -2.2 5,804,524 - BT4405 0.32 -1.2 5,804,644 - BT4405 0.39 -1.1 5,804,645 + BT4405 0.39 -0.5 5,804,675 + BT4405 0.41 -1.4 5,804,711 - BT4405 0.43 -2.7 5,804,829 + BT4405 0.51 +1.5 5,804,836 + BT4405 0.51 +0.2 5,805,071 - BT4405 0.66 -2.1 5,805,223 + BT4405 0.75 +0.3 5,805,276 - BT4405 0.78 +2.9 5,805,285 - BT4405 0.79 -0.4 5,805,301 + BT4405 0.80 -1.6 5,805,301 + BT4405 0.80 +0.8 5,805,493 + +2.5 5,805,501 - -0.5 5,805,501 - +1.8 5,805,592 + +1.8 5,805,666 + +0.2 5,805,668 + -2.9 5,805,668 + -2.2 5,805,670 + +0.3 5,805,684 + -0.6 5,805,779 + BT4406 0.12 -1.1 5,806,001 - BT4406 0.31 +1.5 5,806,030 + BT4406 0.34 -0.3 5,806,140 - BT4406 0.43 -0.1 5,806,292 + BT4406 0.56 +2.1 5,806,297 - BT4406 0.57 +0.5 5,806,410 - BT4406 0.66 -0.6 5,806,416 - BT4406 0.67 -0.3 5,806,466 + BT4406 0.71 -1.5 5,806,494 - BT4406 0.74 -0.3 5,806,551 - BT4406 0.78 +0.2 5,806,564 + BT4406 0.80 +1.0
Or see this region's nucleotide sequence