Experiment: BHIS with Chloride 350 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1596 and BT1597 are separated by 320 nucleotides BT1597 and BT1598 overlap by 4 nucleotides
BT1596: BT1596 - putative sulfatase yidJ (NCBI ptt file), at 1,963,594 to 1,965,039
BT1596
BT1597: BT1597 - two-component system sensor histidine kinase (NCBI ptt file), at 1,965,360 to 1,966,979
BT1597
BT1598: BT1598 - putative two-component system sensor histidine kinase (NCBI ptt file), at 1,966,976 to 1,968,943
BT1598
Position (kb)
1965
1966
1967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1964.495 kb on - strand, within BT1596 at 1964.589 kb on - strand, within BT1596 at 1964.701 kb on + strand, within BT1596 at 1964.734 kb on + strand, within BT1596 at 1964.739 kb on + strand, within BT1596 at 1964.776 kb on - strand, within BT1596 at 1964.779 kb on + strand, within BT1596 at 1964.779 kb on + strand, within BT1596 at 1964.779 kb on + strand, within BT1596 at 1964.780 kb on - strand, within BT1596 at 1964.780 kb on - strand, within BT1596 at 1964.911 kb on + strand at 1964.912 kb on - strand at 1964.952 kb on - strand at 1965.045 kb on + strand at 1965.128 kb on + strand at 1965.129 kb on - strand at 1965.129 kb on - strand at 1965.136 kb on + strand at 1965.136 kb on + strand at 1965.146 kb on - strand at 1965.146 kb on - strand at 1965.148 kb on + strand at 1965.166 kb on + strand at 1965.166 kb on + strand at 1965.215 kb on + strand at 1965.215 kb on + strand at 1965.216 kb on - strand at 1965.216 kb on - strand at 1965.216 kb on - strand at 1965.379 kb on + strand at 1965.449 kb on - strand at 1965.454 kb on - strand at 1965.498 kb on + strand at 1965.499 kb on - strand at 1965.579 kb on - strand, within BT1597 at 1965.585 kb on + strand, within BT1597 at 1965.590 kb on + strand, within BT1597 at 1965.591 kb on - strand, within BT1597 at 1965.681 kb on + strand, within BT1597 at 1965.715 kb on - strand, within BT1597 at 1965.715 kb on - strand, within BT1597 at 1965.729 kb on + strand, within BT1597 at 1965.736 kb on - strand, within BT1597 at 1965.742 kb on - strand, within BT1597 at 1965.742 kb on - strand, within BT1597 at 1965.742 kb on - strand, within BT1597 at 1965.743 kb on + strand, within BT1597 at 1965.744 kb on - strand, within BT1597 at 1965.746 kb on - strand, within BT1597 at 1965.817 kb on - strand, within BT1597 at 1965.864 kb on - strand, within BT1597 at 1965.885 kb on - strand, within BT1597 at 1965.983 kb on - strand, within BT1597 at 1965.986 kb on + strand, within BT1597 at 1965.987 kb on - strand, within BT1597 at 1965.987 kb on - strand, within BT1597 at 1966.029 kb on - strand, within BT1597 at 1966.088 kb on + strand, within BT1597 at 1966.118 kb on + strand, within BT1597 at 1966.133 kb on - strand, within BT1597 at 1966.147 kb on - strand, within BT1597 at 1966.314 kb on - strand, within BT1597 at 1966.314 kb on - strand, within BT1597 at 1966.462 kb on + strand, within BT1597 at 1966.521 kb on - strand, within BT1597 at 1966.525 kb on + strand, within BT1597 at 1966.526 kb on - strand, within BT1597 at 1966.526 kb on - strand, within BT1597 at 1966.730 kb on + strand, within BT1597 at 1966.862 kb on + strand at 1966.958 kb on - strand at 1967.105 kb on - strand at 1967.122 kb on - strand at 1967.141 kb on + strand at 1967.175 kb on + strand, within BT1598 at 1967.204 kb on + strand, within BT1598 at 1967.204 kb on + strand, within BT1598 at 1967.225 kb on + strand, within BT1598 at 1967.226 kb on - strand, within BT1598 at 1967.226 kb on - strand, within BT1598 at 1967.379 kb on - strand, within BT1598 at 1967.460 kb on - strand, within BT1598 at 1967.532 kb on - strand, within BT1598 at 1967.712 kb on + strand, within BT1598 at 1967.788 kb on - strand, within BT1598 at 1967.832 kb on - strand, within BT1598 at 1967.833 kb on + strand, within BT1598 at 1967.856 kb on - strand, within BT1598 at 1967.903 kb on + strand, within BT1598 at 1967.924 kb on - strand, within BT1598 at 1967.924 kb on - strand, within BT1598
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chloride 350 mM remove 1,964,495 - BT1596 0.62 -0.2 1,964,589 - BT1596 0.69 -1.9 1,964,701 + BT1596 0.77 +1.1 1,964,734 + BT1596 0.79 -1.2 1,964,739 + BT1596 0.79 -0.5 1,964,776 - BT1596 0.82 -3.4 1,964,779 + BT1596 0.82 -2.4 1,964,779 + BT1596 0.82 +3.4 1,964,779 + BT1596 0.82 +0.7 1,964,780 - BT1596 0.82 -0.2 1,964,780 - BT1596 0.82 -1.1 1,964,911 + +0.7 1,964,912 - +1.6 1,964,952 - -3.6 1,965,045 + +1.0 1,965,128 + +1.1 1,965,129 - -0.4 1,965,129 - -0.0 1,965,136 + +1.0 1,965,136 + -1.6 1,965,146 - -0.5 1,965,146 - +0.4 1,965,148 + +1.8 1,965,166 + -0.7 1,965,166 + -1.0 1,965,215 + +1.0 1,965,215 + +1.3 1,965,216 - +0.8 1,965,216 - +1.3 1,965,216 - -1.1 1,965,379 + -0.8 1,965,449 - -2.7 1,965,454 - -1.4 1,965,498 + -1.4 1,965,499 - -1.0 1,965,579 - BT1597 0.14 +0.6 1,965,585 + BT1597 0.14 -1.4 1,965,590 + BT1597 0.14 -0.1 1,965,591 - BT1597 0.14 -1.2 1,965,681 + BT1597 0.20 -2.8 1,965,715 - BT1597 0.22 -0.4 1,965,715 - BT1597 0.22 +0.9 1,965,729 + BT1597 0.23 -0.0 1,965,736 - BT1597 0.23 -0.0 1,965,742 - BT1597 0.24 +0.4 1,965,742 - BT1597 0.24 -2.8 1,965,742 - BT1597 0.24 +1.3 1,965,743 + BT1597 0.24 -2.6 1,965,744 - BT1597 0.24 +1.0 1,965,746 - BT1597 0.24 +1.6 1,965,817 - BT1597 0.28 -2.1 1,965,864 - BT1597 0.31 -1.1 1,965,885 - BT1597 0.32 -0.3 1,965,983 - BT1597 0.38 -0.6 1,965,986 + BT1597 0.39 -2.7 1,965,987 - BT1597 0.39 -0.8 1,965,987 - BT1597 0.39 +0.6 1,966,029 - BT1597 0.41 -1.1 1,966,088 + BT1597 0.45 +1.2 1,966,118 + BT1597 0.47 -3.1 1,966,133 - BT1597 0.48 +0.4 1,966,147 - BT1597 0.49 +4.6 1,966,314 - BT1597 0.59 -0.0 1,966,314 - BT1597 0.59 -1.1 1,966,462 + BT1597 0.68 -0.4 1,966,521 - BT1597 0.72 +1.1 1,966,525 + BT1597 0.72 +1.2 1,966,526 - BT1597 0.72 +2.3 1,966,526 - BT1597 0.72 +0.6 1,966,730 + BT1597 0.85 -1.4 1,966,862 + +0.1 1,966,958 - -1.5 1,967,105 - +1.8 1,967,122 - -2.7 1,967,141 + +0.6 1,967,175 + BT1598 0.10 +1.0 1,967,204 + BT1598 0.12 -2.1 1,967,204 + BT1598 0.12 -3.5 1,967,225 + BT1598 0.13 -1.3 1,967,226 - BT1598 0.13 -1.2 1,967,226 - BT1598 0.13 -1.1 1,967,379 - BT1598 0.20 -0.8 1,967,460 - BT1598 0.25 +2.6 1,967,532 - BT1598 0.28 -1.4 1,967,712 + BT1598 0.37 -0.5 1,967,788 - BT1598 0.41 -0.6 1,967,832 - BT1598 0.43 +0.6 1,967,833 + BT1598 0.44 -0.5 1,967,856 - BT1598 0.45 +1.0 1,967,903 + BT1598 0.47 +0.3 1,967,924 - BT1598 0.48 +1.2 1,967,924 - BT1598 0.48 -2.6
Or see this region's nucleotide sequence