Experiment: BHIS with Chloride 350 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0319 and BT0320 are separated by 146 nucleotides BT0320 and BT0321 are separated by 37 nucleotides BT0321 and BT0322 are separated by 121 nucleotides
BT0319: BT0319 - hypothetical protein (NCBI ptt file), at 386,576 to 386,947
BT0319
BT0320: BT0320 - hypothetical protein (NCBI ptt file), at 387,094 to 387,897
BT0320
BT0321: BT0321 - cytidine/deoxycytidylate deaminase (NCBI ptt file), at 387,935 to 388,405
BT0321
BT0322: BT0322 - putative oxidoreductase (NCBI ptt file), at 388,527 to 389,501
BT0322
Position (kb) 387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 386.959 kb on - strand at 387.054 kb on - strand at 387.061 kb on + strand at 387.238 kb on + strand, within BT0320 at 387.436 kb on + strand, within BT0320 at 387.437 kb on - strand, within BT0320 at 387.672 kb on + strand, within BT0320 at 387.673 kb on - strand, within BT0320 at 387.673 kb on - strand, within BT0320 at 387.907 kb on + strand at 387.907 kb on + strand at 387.908 kb on - strand at 387.944 kb on + strand at 387.945 kb on - strand at 388.004 kb on + strand, within BT0321 at 388.111 kb on - strand, within BT0321 at 388.135 kb on - strand, within BT0321 at 388.186 kb on - strand, within BT0321 at 388.229 kb on + strand, within BT0321 at 388.238 kb on - strand, within BT0321 at 388.291 kb on + strand, within BT0321 at 388.405 kb on + strand at 388.477 kb on + strand at 388.478 kb on - strand at 388.524 kb on - strand at 388.529 kb on - strand at 388.620 kb on + strand at 388.740 kb on - strand, within BT0322 at 388.746 kb on - strand, within BT0322 at 388.923 kb on - strand, within BT0322 at 389.058 kb on + strand, within BT0322 at 389.072 kb on + strand, within BT0322 at 389.169 kb on - strand, within BT0322 at 389.175 kb on - strand, within BT0322 at 389.227 kb on + strand, within BT0322 at 389.227 kb on + strand, within BT0322
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chloride 350 mM remove 386,959 - -0.9 387,054 - -1.5 387,061 + +0.8 387,238 + BT0320 0.18 +2.0 387,436 + BT0320 0.43 -1.1 387,437 - BT0320 0.43 -1.7 387,672 + BT0320 0.72 +1.4 387,673 - BT0320 0.72 -0.1 387,673 - BT0320 0.72 +0.4 387,907 + +0.1 387,907 + -0.5 387,908 - +1.9 387,944 + -0.3 387,945 - -2.1 388,004 + BT0321 0.15 -1.1 388,111 - BT0321 0.37 -3.5 388,135 - BT0321 0.42 -1.1 388,186 - BT0321 0.53 -2.0 388,229 + BT0321 0.62 -1.8 388,238 - BT0321 0.64 +1.5 388,291 + BT0321 0.76 -2.2 388,405 + +0.4 388,477 + +0.2 388,478 - -0.1 388,524 - +0.2 388,529 - -1.0 388,620 + -0.1 388,740 - BT0322 0.22 -1.3 388,746 - BT0322 0.22 -0.8 388,923 - BT0322 0.41 -0.8 389,058 + BT0322 0.54 -2.5 389,072 + BT0322 0.56 -1.7 389,169 - BT0322 0.66 +1.2 389,175 - BT0322 0.66 -2.4 389,227 + BT0322 0.72 -1.8 389,227 + BT0322 0.72 -2.3
Or see this region's nucleotide sequence