Experiment: BHIS with Chloride 350 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0229 and BT0230 are separated by 111 nucleotides BT0230 and BT0231 are separated by 37 nucleotides BT0231 and BT0232 are separated by 108 nucleotides
BT0229: BT0229 - conserved hypothetical protein (NCBI ptt file), at 249,577 to 250,602
BT0229
BT0230: BT0230 - conserved hypothetical protein (NCBI ptt file), at 250,714 to 252,072
BT0230
BT0231: BT0231 - excisionase (NCBI ptt file), at 252,110 to 252,481
BT0231
BT0232: BT0232 - putative protein involved in transposition (NCBI ptt file), at 252,590 to 253,357
BT0232
Position (kb) 250
251
252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 249.891 kb on + strand, within BT0229 at 249.892 kb on - strand, within BT0229 at 250.070 kb on + strand, within BT0229 at 250.112 kb on + strand, within BT0229 at 250.113 kb on - strand, within BT0229 at 250.113 kb on - strand, within BT0229 at 250.186 kb on - strand, within BT0229 at 250.187 kb on + strand, within BT0229 at 250.256 kb on + strand, within BT0229 at 250.302 kb on + strand, within BT0229 at 250.320 kb on - strand, within BT0229 at 250.386 kb on + strand, within BT0229 at 250.446 kb on + strand, within BT0229 at 250.505 kb on - strand at 250.505 kb on - strand at 250.568 kb on + strand at 250.619 kb on + strand at 250.660 kb on + strand at 250.812 kb on - strand at 250.898 kb on + strand, within BT0230 at 250.962 kb on - strand, within BT0230 at 250.976 kb on + strand, within BT0230 at 250.976 kb on + strand, within BT0230 at 250.990 kb on + strand, within BT0230 at 251.002 kb on + strand, within BT0230 at 251.068 kb on + strand, within BT0230 at 251.203 kb on - strand, within BT0230 at 251.238 kb on + strand, within BT0230 at 251.249 kb on + strand, within BT0230 at 251.474 kb on - strand, within BT0230 at 251.606 kb on + strand, within BT0230 at 251.866 kb on - strand, within BT0230 at 251.907 kb on + strand, within BT0230 at 251.963 kb on + strand at 251.999 kb on - strand at 252.085 kb on - strand at 252.347 kb on - strand, within BT0231 at 252.350 kb on + strand, within BT0231 at 252.351 kb on - strand, within BT0231 at 252.358 kb on + strand, within BT0231 at 252.653 kb on - strand at 252.906 kb on - strand, within BT0232 at 252.947 kb on - strand, within BT0232 at 252.965 kb on + strand, within BT0232 at 252.966 kb on - strand, within BT0232 at 253.020 kb on - strand, within BT0232
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chloride 350 mM remove 249,891 + BT0229 0.31 +3.1 249,892 - BT0229 0.31 -1.1 250,070 + BT0229 0.48 -0.1 250,112 + BT0229 0.52 -2.1 250,113 - BT0229 0.52 +0.2 250,113 - BT0229 0.52 -0.6 250,186 - BT0229 0.59 +0.5 250,187 + BT0229 0.59 -2.1 250,256 + BT0229 0.66 -0.4 250,302 + BT0229 0.71 -2.1 250,320 - BT0229 0.72 -2.1 250,386 + BT0229 0.79 +1.7 250,446 + BT0229 0.85 -1.8 250,505 - +1.7 250,505 - +2.0 250,568 + +1.2 250,619 + -0.4 250,660 + -0.6 250,812 - -0.5 250,898 + BT0230 0.14 -0.1 250,962 - BT0230 0.18 -0.5 250,976 + BT0230 0.19 -2.4 250,976 + BT0230 0.19 -1.9 250,990 + BT0230 0.20 +0.2 251,002 + BT0230 0.21 -2.9 251,068 + BT0230 0.26 +0.5 251,203 - BT0230 0.36 -0.7 251,238 + BT0230 0.39 +0.5 251,249 + BT0230 0.39 -2.9 251,474 - BT0230 0.56 +0.5 251,606 + BT0230 0.66 -1.6 251,866 - BT0230 0.85 -2.6 251,907 + BT0230 0.88 -2.2 251,963 + -0.2 251,999 - +1.0 252,085 - -1.0 252,347 - BT0231 0.64 -0.1 252,350 + BT0231 0.65 -2.8 252,351 - BT0231 0.65 -1.6 252,358 + BT0231 0.67 +2.7 252,653 - -2.2 252,906 - BT0232 0.41 +0.6 252,947 - BT0232 0.46 -0.3 252,965 + BT0232 0.49 -1.9 252,966 - BT0232 0.49 +0.5 253,020 - BT0232 0.56 -2.3
Or see this region's nucleotide sequence