Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_830 and Pf6N2E2_831 are separated by 57 nucleotides Pf6N2E2_831 and Pf6N2E2_832 are separated by 48 nucleotides Pf6N2E2_832 and Pf6N2E2_833 are separated by 383 nucleotides
Pf6N2E2_830 - Long-chain fatty acid transport protein, at 968,049 to 969,320
_830
Pf6N2E2_831 - Ketoacyl reductase hetN (EC 1.3.1.-), at 969,378 to 970,163
_831
Pf6N2E2_832 - Outer membrane component of tripartite multidrug resistance system, at 970,212 to 971,675
_832
Pf6N2E2_833 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 972,059 to 973,003
_833
Position (kb) 970
971
972 Strain fitness (log2 ratio) -2
-1
0
1
2 at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.258 kb on + strand at 969.259 kb on - strand at 969.259 kb on - strand at 969.296 kb on + strand at 969.297 kb on - strand at 969.680 kb on - strand, within Pf6N2E2_831 at 969.697 kb on + strand, within Pf6N2E2_831 at 969.697 kb on + strand, within Pf6N2E2_831 at 969.698 kb on - strand, within Pf6N2E2_831 at 969.725 kb on - strand, within Pf6N2E2_831 at 970.087 kb on + strand at 970.195 kb on + strand at 970.196 kb on - strand at 970.391 kb on - strand, within Pf6N2E2_832 at 970.437 kb on + strand, within Pf6N2E2_832 at 970.717 kb on + strand, within Pf6N2E2_832 at 970.717 kb on + strand, within Pf6N2E2_832 at 970.718 kb on - strand, within Pf6N2E2_832 at 970.800 kb on + strand, within Pf6N2E2_832 at 970.801 kb on - strand, within Pf6N2E2_832 at 970.814 kb on + strand, within Pf6N2E2_832 at 970.999 kb on + strand, within Pf6N2E2_832 at 970.999 kb on + strand, within Pf6N2E2_832 at 970.999 kb on + strand, within Pf6N2E2_832 at 971.024 kb on - strand, within Pf6N2E2_832 at 971.082 kb on - strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.116 kb on + strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.117 kb on - strand, within Pf6N2E2_832 at 971.263 kb on + strand, within Pf6N2E2_832 at 971.263 kb on + strand, within Pf6N2E2_832 at 971.263 kb on + strand, within Pf6N2E2_832 at 971.263 kb on + strand, within Pf6N2E2_832 at 971.264 kb on - strand, within Pf6N2E2_832 at 971.360 kb on + strand, within Pf6N2E2_832 at 971.360 kb on + strand, within Pf6N2E2_832 at 971.361 kb on - strand, within Pf6N2E2_832 at 971.534 kb on + strand at 971.602 kb on + strand at 971.603 kb on - strand at 971.646 kb on + strand at 971.762 kb on + strand at 971.763 kb on - strand at 971.763 kb on - strand at 971.772 kb on + strand at 971.893 kb on + strand at 971.893 kb on + strand at 971.893 kb on + strand at 971.893 kb on + strand at 971.893 kb on + strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 971.894 kb on - strand at 972.399 kb on - strand, within Pf6N2E2_833 at 972.649 kb on + strand, within Pf6N2E2_833 at 972.650 kb on - strand, within Pf6N2E2_833 at 972.665 kb on + strand, within Pf6N2E2_833 at 972.666 kb on - strand, within Pf6N2E2_833
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 969,258 + -1.2 969,258 + +0.1 969,258 + +0.1 969,258 + -0.1 969,258 + +0.4 969,259 - +0.6 969,259 - +0.8 969,296 + -0.2 969,297 - +0.6 969,680 - Pf6N2E2_831 0.38 +0.2 969,697 + Pf6N2E2_831 0.41 -1.0 969,697 + Pf6N2E2_831 0.41 +0.9 969,698 - Pf6N2E2_831 0.41 -0.5 969,725 - Pf6N2E2_831 0.44 +0.4 970,087 + -0.4 970,195 + -1.0 970,196 - +0.3 970,391 - Pf6N2E2_832 0.12 -0.3 970,437 + Pf6N2E2_832 0.15 +0.2 970,717 + Pf6N2E2_832 0.34 -0.3 970,717 + Pf6N2E2_832 0.34 -0.1 970,718 - Pf6N2E2_832 0.35 -0.9 970,800 + Pf6N2E2_832 0.40 -1.0 970,801 - Pf6N2E2_832 0.40 -0.1 970,814 + Pf6N2E2_832 0.41 -0.3 970,999 + Pf6N2E2_832 0.54 +2.0 970,999 + Pf6N2E2_832 0.54 +0.2 970,999 + Pf6N2E2_832 0.54 -0.6 971,024 - Pf6N2E2_832 0.55 -0.2 971,082 - Pf6N2E2_832 0.59 +0.1 971,116 + Pf6N2E2_832 0.62 +0.1 971,116 + Pf6N2E2_832 0.62 +0.0 971,116 + Pf6N2E2_832 0.62 -0.0 971,116 + Pf6N2E2_832 0.62 +1.1 971,116 + Pf6N2E2_832 0.62 -2.1 971,116 + Pf6N2E2_832 0.62 +0.7 971,116 + Pf6N2E2_832 0.62 +0.0 971,116 + Pf6N2E2_832 0.62 -1.0 971,116 + Pf6N2E2_832 0.62 -0.1 971,117 - Pf6N2E2_832 0.62 +1.0 971,117 - Pf6N2E2_832 0.62 +0.7 971,117 - Pf6N2E2_832 0.62 -0.9 971,117 - Pf6N2E2_832 0.62 +0.5 971,117 - Pf6N2E2_832 0.62 +0.2 971,117 - Pf6N2E2_832 0.62 +0.6 971,117 - Pf6N2E2_832 0.62 +0.2 971,117 - Pf6N2E2_832 0.62 -0.2 971,117 - Pf6N2E2_832 0.62 +0.1 971,117 - Pf6N2E2_832 0.62 +0.6 971,263 + Pf6N2E2_832 0.72 -0.8 971,263 + Pf6N2E2_832 0.72 -0.2 971,263 + Pf6N2E2_832 0.72 -0.5 971,263 + Pf6N2E2_832 0.72 +1.3 971,264 - Pf6N2E2_832 0.72 -0.3 971,360 + Pf6N2E2_832 0.78 -0.3 971,360 + Pf6N2E2_832 0.78 -0.7 971,361 - Pf6N2E2_832 0.78 -1.0 971,534 + +0.5 971,602 + +0.9 971,603 - -0.3 971,646 + -0.4 971,762 + -0.4 971,763 - +1.2 971,763 - +1.1 971,772 + -0.4 971,893 + -0.9 971,893 + +1.8 971,893 + -0.8 971,893 + -0.5 971,893 + -0.7 971,894 - -0.4 971,894 - +0.6 971,894 - +0.0 971,894 - +0.3 971,894 - +0.7 971,894 - -1.0 971,894 - +0.1 971,894 - +0.5 972,399 - Pf6N2E2_833 0.36 -0.8 972,649 + Pf6N2E2_833 0.62 -1.0 972,650 - Pf6N2E2_833 0.63 -0.0 972,665 + Pf6N2E2_833 0.64 +0.7 972,666 - Pf6N2E2_833 0.64 +0.6
Or see this region's nucleotide sequence