Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_691 and Pf6N2E2_692 overlap by 4 nucleotides Pf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotides Pf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotides
Pf6N2E2_691 - D-galactarate permease, at 797,045 to 798,442
_691
Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713
_692
Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125
_693
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Position (kb) 798
799
800 Strain fitness (log2 ratio) -2
-1
0
1
2 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.444 kb on + strand, within Pf6N2E2_691 at 797.445 kb on - strand, within Pf6N2E2_691 at 797.445 kb on - strand, within Pf6N2E2_691 at 797.447 kb on + strand, within Pf6N2E2_691 at 797.463 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.840 kb on + strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.841 kb on - strand, within Pf6N2E2_691 at 797.951 kb on + strand, within Pf6N2E2_691 at 798.140 kb on + strand, within Pf6N2E2_691 at 798.269 kb on + strand, within Pf6N2E2_691 at 798.439 kb on - strand at 798.439 kb on - strand at 798.562 kb on + strand at 798.562 kb on + strand at 798.563 kb on - strand at 798.563 kb on - strand at 798.583 kb on + strand, within Pf6N2E2_692 at 798.672 kb on + strand, within Pf6N2E2_692 at 798.892 kb on + strand, within Pf6N2E2_692 at 798.991 kb on + strand, within Pf6N2E2_692 at 799.110 kb on + strand, within Pf6N2E2_692 at 799.110 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.927 kb on - strand at 799.952 kb on + strand at 799.952 kb on + strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.132 kb on - strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.222 kb on + strand at 800.258 kb on + strand, within Pf6N2E2_694 at 800.273 kb on + strand, within Pf6N2E2_694 at 800.274 kb on - strand, within Pf6N2E2_694 at 800.309 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.480 kb on + strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 797,444 + Pf6N2E2_691 0.29 +0.1 797,444 + Pf6N2E2_691 0.29 +0.4 797,444 + Pf6N2E2_691 0.29 +0.1 797,445 - Pf6N2E2_691 0.29 +0.4 797,445 - Pf6N2E2_691 0.29 -0.2 797,447 + Pf6N2E2_691 0.29 +0.4 797,463 + Pf6N2E2_691 0.30 +0.9 797,840 + Pf6N2E2_691 0.57 -0.7 797,840 + Pf6N2E2_691 0.57 -2.5 797,840 + Pf6N2E2_691 0.57 +1.9 797,840 + Pf6N2E2_691 0.57 -0.7 797,840 + Pf6N2E2_691 0.57 +0.3 797,840 + Pf6N2E2_691 0.57 +0.6 797,840 + Pf6N2E2_691 0.57 -2.7 797,840 + Pf6N2E2_691 0.57 +1.4 797,841 - Pf6N2E2_691 0.57 -0.6 797,841 - Pf6N2E2_691 0.57 +0.3 797,841 - Pf6N2E2_691 0.57 +0.7 797,841 - Pf6N2E2_691 0.57 +0.8 797,841 - Pf6N2E2_691 0.57 -0.6 797,841 - Pf6N2E2_691 0.57 +1.2 797,841 - Pf6N2E2_691 0.57 +0.3 797,951 + Pf6N2E2_691 0.65 +0.1 798,140 + Pf6N2E2_691 0.78 -0.1 798,269 + Pf6N2E2_691 0.88 -0.1 798,439 - -0.0 798,439 - +0.7 798,562 + +2.1 798,562 + +1.0 798,563 - -0.2 798,563 - +1.2 798,583 + Pf6N2E2_692 0.11 -0.9 798,672 + Pf6N2E2_692 0.18 -0.9 798,892 + Pf6N2E2_692 0.36 -0.2 798,991 + Pf6N2E2_692 0.43 +0.1 799,110 + Pf6N2E2_692 0.53 -0.5 799,110 + Pf6N2E2_692 0.53 -0.7 799,168 + Pf6N2E2_692 0.57 -0.4 799,168 + Pf6N2E2_692 0.57 -2.3 799,927 - +0.2 799,952 + -0.7 799,952 + +0.6 799,953 - -0.4 799,953 - -1.3 799,953 - +0.6 799,990 - -1.7 799,990 - +0.5 799,990 - +0.3 800,055 - Pf6N2E2_693 0.49 +0.6 800,055 - Pf6N2E2_693 0.49 +2.3 800,055 - Pf6N2E2_693 0.49 +0.0 800,132 - -0.2 800,169 + +1.0 800,169 + -0.7 800,169 + +0.8 800,169 + -0.2 800,169 + +0.0 800,170 - +0.2 800,170 - +1.7 800,170 - -0.7 800,170 - +1.3 800,222 + -0.7 800,258 + Pf6N2E2_694 0.11 +0.1 800,273 + Pf6N2E2_694 0.12 +0.4 800,274 - Pf6N2E2_694 0.12 +0.7 800,309 + Pf6N2E2_694 0.15 -0.5 800,354 + Pf6N2E2_694 0.18 +1.5 800,354 + Pf6N2E2_694 0.18 +0.0 800,355 - Pf6N2E2_694 0.18 -1.2 800,355 - Pf6N2E2_694 0.18 -1.0 800,355 - Pf6N2E2_694 0.18 +0.1 800,355 - Pf6N2E2_694 0.18 -0.4 800,480 + Pf6N2E2_694 0.28 +0.6 800,481 - Pf6N2E2_694 0.28 +0.7 800,481 - Pf6N2E2_694 0.28 +0.2 800,481 - Pf6N2E2_694 0.28 -1.6 800,481 - Pf6N2E2_694 0.28 +0.2
Or see this region's nucleotide sequence