Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6079 and Pf6N2E2_6080 are separated by 32 nucleotides Pf6N2E2_6080 and Pf6N2E2_6081 are separated by 90 nucleotides Pf6N2E2_6081 and Pf6N2E2_6082 are separated by 6 nucleotides Pf6N2E2_6082 and Pf6N2E2_6083 are separated by 168 nucleotides
Pf6N2E2_6079 - 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent, at 6,899,417 to 6,900,007
_6079
Pf6N2E2_6080 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 6,900,040 to 6,900,768
_6080
Pf6N2E2_6081 - Transcriptional regulator, TetR family, in cluster with 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent, at 6,900,859 to 6,901,428
_6081
Pf6N2E2_6082 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 6,901,435 to 6,901,716
_6082
Pf6N2E2_6083 - Threonine and homoserine efflux system, at 6,901,885 to 6,902,775
_6083
Position (kb)
6900
6901
6902 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6899.888 kb on + strand, within Pf6N2E2_6079 at 6899.958 kb on + strand at 6899.985 kb on + strand at 6900.041 kb on + strand at 6900.042 kb on - strand at 6900.110 kb on + strand at 6900.110 kb on + strand at 6900.110 kb on + strand at 6900.116 kb on + strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.117 kb on - strand, within Pf6N2E2_6080 at 6900.171 kb on - strand, within Pf6N2E2_6080 at 6900.171 kb on - strand, within Pf6N2E2_6080 at 6900.243 kb on + strand, within Pf6N2E2_6080 at 6900.455 kb on + strand, within Pf6N2E2_6080 at 6900.455 kb on + strand, within Pf6N2E2_6080 at 6900.455 kb on + strand, within Pf6N2E2_6080 at 6900.749 kb on - strand at 6901.000 kb on + strand, within Pf6N2E2_6081 at 6901.129 kb on + strand, within Pf6N2E2_6081 at 6901.129 kb on + strand, within Pf6N2E2_6081 at 6901.130 kb on - strand, within Pf6N2E2_6081 at 6901.271 kb on + strand, within Pf6N2E2_6081 at 6901.396 kb on - strand at 6901.436 kb on + strand at 6901.440 kb on + strand at 6901.467 kb on + strand, within Pf6N2E2_6082 at 6901.467 kb on + strand, within Pf6N2E2_6082 at 6901.544 kb on + strand, within Pf6N2E2_6082 at 6901.544 kb on + strand, within Pf6N2E2_6082 at 6901.544 kb on + strand, within Pf6N2E2_6082 at 6901.544 kb on + strand, within Pf6N2E2_6082 at 6901.687 kb on + strand, within Pf6N2E2_6082 at 6901.726 kb on + strand at 6901.726 kb on + strand at 6901.726 kb on + strand at 6901.726 kb on + strand at 6901.727 kb on - strand at 6901.727 kb on - strand at 6901.727 kb on - strand at 6901.727 kb on - strand at 6901.728 kb on + strand at 6901.728 kb on + strand at 6901.729 kb on - strand at 6901.755 kb on - strand at 6901.755 kb on - strand at 6901.755 kb on - strand at 6901.755 kb on - strand at 6901.834 kb on + strand at 6901.860 kb on + strand at 6901.860 kb on + strand at 6901.860 kb on + strand at 6901.861 kb on - strand at 6901.963 kb on - strand at 6901.991 kb on + strand, within Pf6N2E2_6083 at 6901.991 kb on + strand, within Pf6N2E2_6083 at 6901.991 kb on + strand, within Pf6N2E2_6083 at 6901.992 kb on - strand, within Pf6N2E2_6083 at 6901.992 kb on - strand, within Pf6N2E2_6083 at 6901.992 kb on - strand, within Pf6N2E2_6083 at 6901.992 kb on - strand, within Pf6N2E2_6083 at 6901.992 kb on - strand, within Pf6N2E2_6083 at 6902.102 kb on + strand, within Pf6N2E2_6083 at 6902.102 kb on + strand, within Pf6N2E2_6083 at 6902.103 kb on - strand, within Pf6N2E2_6083 at 6902.222 kb on - strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.282 kb on + strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.283 kb on - strand, within Pf6N2E2_6083 at 6902.362 kb on + strand, within Pf6N2E2_6083 at 6902.364 kb on + strand, within Pf6N2E2_6083 at 6902.364 kb on + strand, within Pf6N2E2_6083 at 6902.364 kb on + strand, within Pf6N2E2_6083 at 6902.365 kb on - strand, within Pf6N2E2_6083
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 6,899,888 + Pf6N2E2_6079 0.80 -0.3 6,899,958 + -0.4 6,899,985 + -0.5 6,900,041 + -1.4 6,900,042 - +0.8 6,900,110 + +0.1 6,900,110 + +0.2 6,900,110 + -0.8 6,900,116 + Pf6N2E2_6080 0.10 +0.2 6,900,117 - Pf6N2E2_6080 0.11 +0.1 6,900,117 - Pf6N2E2_6080 0.11 +0.8 6,900,117 - Pf6N2E2_6080 0.11 -0.5 6,900,117 - Pf6N2E2_6080 0.11 -0.3 6,900,171 - Pf6N2E2_6080 0.18 +0.1 6,900,171 - Pf6N2E2_6080 0.18 +2.3 6,900,243 + Pf6N2E2_6080 0.28 +0.9 6,900,455 + Pf6N2E2_6080 0.57 +0.8 6,900,455 + Pf6N2E2_6080 0.57 -0.3 6,900,455 + Pf6N2E2_6080 0.57 +0.9 6,900,749 - +1.2 6,901,000 + Pf6N2E2_6081 0.25 -0.7 6,901,129 + Pf6N2E2_6081 0.47 +0.1 6,901,129 + Pf6N2E2_6081 0.47 -0.8 6,901,130 - Pf6N2E2_6081 0.48 +0.3 6,901,271 + Pf6N2E2_6081 0.72 -1.9 6,901,396 - +2.2 6,901,436 + -1.6 6,901,440 + +0.7 6,901,467 + Pf6N2E2_6082 0.11 +0.7 6,901,467 + Pf6N2E2_6082 0.11 +0.6 6,901,544 + Pf6N2E2_6082 0.39 +1.4 6,901,544 + Pf6N2E2_6082 0.39 +0.1 6,901,544 + Pf6N2E2_6082 0.39 +0.7 6,901,544 + Pf6N2E2_6082 0.39 +0.5 6,901,687 + Pf6N2E2_6082 0.89 +0.1 6,901,726 + +0.1 6,901,726 + +0.3 6,901,726 + +0.7 6,901,726 + +0.6 6,901,727 - +0.6 6,901,727 - -0.7 6,901,727 - -0.1 6,901,727 - -0.6 6,901,728 + +0.3 6,901,728 + +0.4 6,901,729 - -0.1 6,901,755 - -3.1 6,901,755 - +0.3 6,901,755 - -0.4 6,901,755 - -1.4 6,901,834 + +0.3 6,901,860 + +0.7 6,901,860 + -0.8 6,901,860 + +1.1 6,901,861 - -1.6 6,901,963 - -1.3 6,901,991 + Pf6N2E2_6083 0.12 +0.4 6,901,991 + Pf6N2E2_6083 0.12 +0.4 6,901,991 + Pf6N2E2_6083 0.12 +0.9 6,901,992 - Pf6N2E2_6083 0.12 -0.3 6,901,992 - Pf6N2E2_6083 0.12 -0.0 6,901,992 - Pf6N2E2_6083 0.12 -2.0 6,901,992 - Pf6N2E2_6083 0.12 -0.1 6,901,992 - Pf6N2E2_6083 0.12 -0.3 6,902,102 + Pf6N2E2_6083 0.24 -0.5 6,902,102 + Pf6N2E2_6083 0.24 +0.5 6,902,103 - Pf6N2E2_6083 0.24 -0.9 6,902,222 - Pf6N2E2_6083 0.38 +0.7 6,902,282 + Pf6N2E2_6083 0.45 -0.6 6,902,282 + Pf6N2E2_6083 0.45 -0.2 6,902,282 + Pf6N2E2_6083 0.45 -0.0 6,902,282 + Pf6N2E2_6083 0.45 -0.0 6,902,282 + Pf6N2E2_6083 0.45 +0.8 6,902,282 + Pf6N2E2_6083 0.45 +0.3 6,902,283 - Pf6N2E2_6083 0.45 +0.4 6,902,283 - Pf6N2E2_6083 0.45 +0.3 6,902,283 - Pf6N2E2_6083 0.45 -3.3 6,902,283 - Pf6N2E2_6083 0.45 -2.7 6,902,283 - Pf6N2E2_6083 0.45 +0.3 6,902,283 - Pf6N2E2_6083 0.45 -0.1 6,902,362 + Pf6N2E2_6083 0.54 -0.7 6,902,364 + Pf6N2E2_6083 0.54 -0.4 6,902,364 + Pf6N2E2_6083 0.54 -0.1 6,902,364 + Pf6N2E2_6083 0.54 +0.2 6,902,365 - Pf6N2E2_6083 0.54 +0.2
Or see this region's nucleotide sequence