Experiment: Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_568 and Pf6N2E2_569 are separated by 197 nucleotides Pf6N2E2_569 and Pf6N2E2_570 are separated by 127 nucleotides Pf6N2E2_570 and Pf6N2E2_571 are separated by 23 nucleotides
Pf6N2E2_568 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 643,266 to 643,871
_568
Pf6N2E2_569 - FIG00793915: hypothetical protein, at 644,069 to 644,557
_569
Pf6N2E2_570 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 644,685 to 645,302
_570
Pf6N2E2_571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 645,326 to 646,369
_571
Position (kb) 644
645
646 Strain fitness (log2 ratio) -1
0
1 at 643.693 kb on - strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.870 kb on - strand at 643.882 kb on + strand at 643.883 kb on - strand at 643.895 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.922 kb on - strand at 643.947 kb on + strand at 643.947 kb on + strand at 644.053 kb on - strand at 644.070 kb on + strand at 644.070 kb on + strand at 644.070 kb on + strand at 644.581 kb on - strand at 644.581 kb on - strand at 644.711 kb on - strand at 644.711 kb on - strand at 644.864 kb on - strand, within Pf6N2E2_570 at 644.864 kb on - strand, within Pf6N2E2_570 at 644.871 kb on + strand, within Pf6N2E2_570 at 645.157 kb on + strand, within Pf6N2E2_570 at 645.158 kb on - strand, within Pf6N2E2_570 at 645.307 kb on + strand at 645.308 kb on - strand at 645.374 kb on + strand at 645.721 kb on - strand, within Pf6N2E2_571 at 645.725 kb on + strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml remove 643,693 - Pf6N2E2_568 0.70 -1.0 643,734 + Pf6N2E2_568 0.77 -0.3 643,734 + Pf6N2E2_568 0.77 +0.2 643,870 - -0.1 643,882 + -0.7 643,883 - -0.1 643,895 + +0.4 643,921 + +0.4 643,921 + +0.9 643,921 + +1.1 643,921 + -0.1 643,922 - +0.4 643,947 + +0.6 643,947 + -0.1 644,053 - +0.2 644,070 + +0.3 644,070 + +1.2 644,070 + +0.0 644,581 - -0.5 644,581 - -0.9 644,711 - -0.6 644,711 - -0.1 644,864 - Pf6N2E2_570 0.29 -0.7 644,864 - Pf6N2E2_570 0.29 +0.1 644,871 + Pf6N2E2_570 0.30 +1.2 645,157 + Pf6N2E2_570 0.76 +0.5 645,158 - Pf6N2E2_570 0.77 -0.4 645,307 + +1.3 645,308 - +1.2 645,374 + -0.4 645,721 - Pf6N2E2_571 0.38 +0.2 645,725 + Pf6N2E2_571 0.38 +0.0 645,726 - Pf6N2E2_571 0.38 -0.1 645,726 - Pf6N2E2_571 0.38 +1.4 645,726 - Pf6N2E2_571 0.38 +0.3 645,726 - Pf6N2E2_571 0.38 -0.4 645,726 - Pf6N2E2_571 0.38 +0.8 646,015 - Pf6N2E2_571 0.66 -1.3 646,015 - Pf6N2E2_571 0.66 +0.4 646,015 - Pf6N2E2_571 0.66 -1.5 646,298 - +0.5
Or see this region's nucleotide sequence