Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5534

Experiment: Nalidixic 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5533 and Pf6N2E2_5534 are separated by 120 nucleotidesPf6N2E2_5534 and Pf6N2E2_5535 are separated by 181 nucleotidesPf6N2E2_5535 and Pf6N2E2_5536 are separated by 113 nucleotides Pf6N2E2_5533 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 6,349,048 to 6,350,823 _5533 Pf6N2E2_5534 - FIG000859: hypothetical protein YebC, at 6,350,944 to 6,351,690 _5534 Pf6N2E2_5535 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 6,351,872 to 6,352,396 _5535 Pf6N2E2_5536 - Holliday junction DNA helicase RuvA, at 6,352,510 to 6,353,118 _5536 Position (kb) 6350 6351 6352Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 6350.876 kb on + strandat 6350.876 kb on + strandat 6350.877 kb on - strandat 6350.901 kb on - strandat 6351.202 kb on + strand, within Pf6N2E2_5534at 6351.202 kb on + strand, within Pf6N2E2_5534at 6351.202 kb on + strand, within Pf6N2E2_5534at 6351.202 kb on + strand, within Pf6N2E2_5534at 6351.203 kb on - strand, within Pf6N2E2_5534at 6351.203 kb on - strand, within Pf6N2E2_5534at 6351.268 kb on - strand, within Pf6N2E2_5534at 6351.484 kb on + strand, within Pf6N2E2_5534at 6351.484 kb on + strand, within Pf6N2E2_5534at 6351.484 kb on + strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.485 kb on - strand, within Pf6N2E2_5534at 6351.847 kb on - strandat 6351.855 kb on + strandat 6351.872 kb on - strandat 6351.879 kb on + strandat 6352.572 kb on - strand, within Pf6N2E2_5536

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.008 mg/ml
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6,350,876 + -1.6
6,350,876 + -0.3
6,350,877 - -1.8
6,350,901 - -2.5
6,351,202 + Pf6N2E2_5534 0.35 -1.1
6,351,202 + Pf6N2E2_5534 0.35 -1.3
6,351,202 + Pf6N2E2_5534 0.35 -1.9
6,351,202 + Pf6N2E2_5534 0.35 -2.5
6,351,203 - Pf6N2E2_5534 0.35 -3.7
6,351,203 - Pf6N2E2_5534 0.35 -2.5
6,351,268 - Pf6N2E2_5534 0.43 -1.8
6,351,484 + Pf6N2E2_5534 0.72 -3.8
6,351,484 + Pf6N2E2_5534 0.72 -1.5
6,351,484 + Pf6N2E2_5534 0.72 -2.5
6,351,485 - Pf6N2E2_5534 0.72 -2.4
6,351,485 - Pf6N2E2_5534 0.72 -3.3
6,351,485 - Pf6N2E2_5534 0.72 -2.2
6,351,485 - Pf6N2E2_5534 0.72 -2.9
6,351,485 - Pf6N2E2_5534 0.72 -2.7
6,351,485 - Pf6N2E2_5534 0.72 -2.7
6,351,847 - -2.9
6,351,855 + -0.1
6,351,872 - -1.0
6,351,879 + -2.5
6,352,572 - Pf6N2E2_5536 0.10 -2.5

Or see this region's nucleotide sequence